LOCUS       sequence-366416-       11633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses TERT K78E in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Laprade H, Querido E, Smith MJ, Guerit D, Crimmins H, Conomos D, 
            Pourret E, Chartrand P, Sfeir A
  TITLE     Single-Molecule Imaging of Telomerase RNA Reveals a 
            Recruitment-Retention Model for Telomere Elongation.
  JOURNAL   Mol Cell. 2020 Jul 2;79(1):115-126.e6. doi: 
            10.1016/j.molcel.2020.05.005. Epub 2020 Jun 3.
  PUBMED    32497497
REFERENCE   2  (bases 1 to 11633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.05.005"; journalName: "Mol Cell"; date: 
            "2020-07-2- 2"; volume: "79"; issue: "1"; pages: "115-126.e6"
COMMENT     Sequence Label: pHage2-EF1a-TERT K78E-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..11633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        184..1362
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          415..1353
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1310..1330
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1487..4885
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLEELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     misc_feature    4936..5397
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4992..5009)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5219..5238
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5402..6001
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5402..5421)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5898..5918
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6036..6624
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6089..6109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6507..6518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6772..6952
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6999..7017)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7085..7189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7190..7258,7259..8050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7408..7427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8221..8809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8963..8980
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9220..9241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9256..9261,9262..9279,9280..9286)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9294..9310
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9299..9321
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9318..9334
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9318..9334
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9384..9403
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9595..10228
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10275..10400
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10897..11130
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11315..11359
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggactagtcg
      181 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
      241 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      301 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      361 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
      421 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
      481 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      541 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      601 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      661 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      721 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      781 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      841 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      901 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      961 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     1021 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     1081 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     1141 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1201 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1261 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1321 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaagcggccg cttaattaag
     1381 tttaaacgga tcccgccggc ggctagcgtt aacacgcgtt gtacatttta cgtaggaatt
     1441 cgtcctgctg cgcacgtggg aagccctggc cccggccacc cccgcgatgc cgcgcgctcc
     1501 ccgctgccga gccgtgcgct ccctgctgcg cagccactac cgcgaggtgc tgccgctggc
     1561 cacgttcgtg cggcgcctgg ggccccaggg ctggcggctg gtgcagcgcg gggacccggc
     1621 ggctttccgc gcgctggtgg cccagtgcct ggtgtgcgtg ccctgggacg cacggccgcc
     1681 ccccgccgcc ccctccttcc gacaggtgtc ctgcctggag gagctggtgg cccgagtgct
     1741 gcagaggctg tgcgagcgcg gcgcgaagaa cgtgctggcc ttcggcttcg cgctgctgga
     1801 cggggcccgc gggggccccc ccgaggcctt caccaccagc gtgcgcagct acctgcccaa
     1861 cacggtgacc gacgcactgc gggggagcgg ggcgtggggg ctgctgctgc gccgcgtggg
     1921 cgacgacgtg ctggttcacc tgctggcacg ctgcgcgctc tttgtgctgg tggctcccag
     1981 ctgcgcctac caggtgtgcg ggccgccgct gtaccagctc ggcgctgcca ctcaggcccg
     2041 gcccccgcca cacgctagtg gaccccgaag gcgtctggga tgcgaacggg cctggaacca
     2101 tagcgtcagg gaggccgggg tccccctggg cctgccagcc ccgggtgcga ggaggcgcgg
     2161 gggcagtgcc agccgaagtc tgccgttgcc caagaggccc aggcgtggcg ctgcccctga
     2221 gccggagcgg acgcccgttg ggcaggggtc ctgggcccac ccgggcagga cgcgtggacc
     2281 gagtgaccgt ggtttctgtg tggtgtcacc tgccagaccc gccgaagaag ccacctcttt
     2341 ggagggtgcg ctctctggca cgcgccactc ccacccatcc gtgggccgcc agcaccacgc
     2401 gggcccccca tccacatcgc ggccaccacg tccctgggac acgccttgtc ccccggtgta
     2461 cgccgagacc aagcacttcc tctactcctc aggcgacaag gagcagctgc ggccctcctt
     2521 cctactcagc tctctgaggc ccagcctgac tggcgctcgg aggctcgtgg agaccatctt
     2581 tctgggttcc aggccctgga tgccagggac tccccgcagg ttgccccgcc tgccccagcg
     2641 ctactggcaa atgcggcccc tgtttctgga gctgcttggg aaccacgcgc agtgccccta
     2701 cggggtgctc ctcaagacgc actgcccgct gcgagctgcg gtcaccccag cagccggtgt
     2761 ctgtgcccgg gagaagcccc agggctctgt ggcggccccc gaggaggagg acacagaccc
     2821 ccgtcgcctg gtgcagctgc tccgccagca cagcagcccc tggcaggtgt acggcttcgt
     2881 gcgggcctgc ctgcgccggc tggtgccccc aggcctctgg ggctccaggc acaacgaacg
     2941 ccgcttcctc aggaacacca agaagttcat ctccctgggg aagcatgcca agctctcgct
     3001 gcaggagctg acgtggaaga tgagcgtgcg gggctgcgct tggctgcgca ggagcccagg
     3061 ggttggctgt gttccggccg cagagcaccg tctgcgtgag gagatcctgg ccaagttcct
     3121 gcactggctg atgagtgtgt acgtcgtcga gctgctcagg tctttctttt atgtcacgga
     3181 gaccacgttt caaaagaaca ggctcttttt ctaccggaag agtgtctgga gcaagttgca
     3241 aagcattgga atcagacagc acttgaagag ggtgcagctg cgggagctgt cggaagcaga
     3301 ggtcaggcag catcgggaag ccaggcccgc cctgctgacg tccagactcc gcttcatccc
     3361 caagcctgac gggctgcggc cgattgtgaa catggactac gtcgtgggag ccagaacgtt
     3421 ccgcagagaa aagagggccg agcgtctcac ctcgagggtg aaggcactgt tcagcgtgct
     3481 caactacgag cgggcgcggc gccccggcct cctgggcgcc tctgtgctgg gcctggacga
     3541 tatccacagg gcctggcgca ccttcgtgct gcgtgtgcgg gcccaggacc cgccgcctga
     3601 gctgtacttt gtcaaggtgg atgtgacggg cgcgtacgac accatccccc aggacaggct
     3661 cacggaggtc atcgccagca tcatcaaacc ccagaacacg tactgcgtgc gtcggtatgc
     3721 cgtggtccag aaggccgccc atgggcacgt ccgcaaggcc ttcaagagcc acgtctctac
     3781 cttgacagac ctccagccgt acatgcgaca gttcgtggct cacctgcagg agaccagccc
     3841 gctgagggat gccgtcgtca tcgagcagag ctcctccctg aatgaggcca gcagtggcct
     3901 cttcgacgtc ttcctacgct tcatgtgcca ccacgccgtg cgcatcaggg gcaagtccta
     3961 cgtccagtgc caggggatcc cgcagggctc catcctctcc acgctgctct gcagcctgtg
     4021 ctacggcgac atggagaaca agctgtttgc ggggattcgg cgggacgggc tgctcctgcg
     4081 tttggtggat gatttcttgt tggtgacacc tcacctcacc cacgcgaaaa ccttcctcag
     4141 gaccctggtc cgaggtgtcc ctgagtatgg ctgcgtggtg aacttgcgga agacagtggt
     4201 gaacttccct gtagaagacg aggccctggg tggcacggct tttgttcaga tgccggccca
     4261 cggcctattc ccctggtgcg gcctgctgct ggatacccgg accctggagg tgcagagcga
     4321 ctactccagc tatgcccgga cctccatcag agccagtctc accttcaacc gcggcttcaa
     4381 ggctgggagg aacatgcgtc gcaaactctt tggggtcttg cggctgaagt gtcacagcct
     4441 gtttctggat ttgcaggtga acagcctcca gacggtgtgc accaacatct acaagatcct
     4501 cctgctgcag gcgtacaggt ttcacgcatg tgtgctgcag ctcccatttc atcagcaagt
     4561 ttggaagaac cccacatttt tcctgcgcgt catctctgac acggcctccc tctgctactc
     4621 catcctgaaa gccaagaacg cagggatgtc gctgggggcc aagggcgccg ccggccctct
     4681 gccctccgag gccgtgcagt ggctgtgcca ccaagcattc ctgctcaagc tgactcgaca
     4741 ccgtgtcacc tacgtgccac tcctggggtc actcaggaca gcccagacgc agctgagtcg
     4801 gaagctcccg gggacgacgc tgactgccct ggaggccgca gccaacccgg cactgccctc
     4861 agacttcaag accatcctgg actgagtcga ccaattccgg ttattttcca ccatattgcc
     4921 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     4981 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     5041 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     5101 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     5161 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     5221 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     5281 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     5341 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatac
     5401 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     5461 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     5521 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     5581 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     5641 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     5701 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     5761 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     5821 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     5881 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     5941 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6001 aagtagatgc cgaccgggat ctatcgatag atcctaatca acctctggat tacaaaattt
     6061 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6121 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6181 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6241 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6301 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6361 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6421 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     6481 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6541 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6601 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     6661 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6721 cgaagacaag atcacctgca ggacaggcgc gccctgcttt ttgcttgtac tgggtctctc
     6781 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6841 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6901 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cacccgggcg
     6961 attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt
     7021 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     7081 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7141 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7201 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7261 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7321 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7381 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     7441 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7501 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7561 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7621 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7681 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7741 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7801 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7861 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7921 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7981 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8041 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8101 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8161 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8221 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8281 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8341 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8401 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8461 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8521 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8581 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8641 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8701 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8761 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8821 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8881 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8941 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9001 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
     9061 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9121 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
     9181 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9241 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9301 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     9361 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9421 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     9481 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     9541 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     9601 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     9661 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     9721 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     9781 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     9841 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     9901 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     9961 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
    10021 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
    10081 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10141 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10201 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
    10261 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
    10321 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
    10381 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
    10441 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
    10501 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
    10561 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
    10621 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
    10681 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
    10741 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    10801 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    10861 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    10921 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    10981 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    11041 gcatctgttg caactcacag tctggggcat caagcagctt caggcaagaa tcctggctgt
    11101 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    11161 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    11221 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    11281 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    11341 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    11401 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    11461 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    11521 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
    11581 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca aat
//
