LOCUS       sequence-366430-        3738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Sepers JJ, Verstappen NHM, Vo AA, Ragle JM, Ruijtenberg S, Ward JD, 
            Boxem M
  TITLE     The mIAA7 degron improves auxin-mediated degradation in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222.
  PUBMED    36029236
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222."
COMMENT     Sequence Label: pJW2341
FEATURES             Location/Qualifiers
     source          1..3738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1539..1559
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1860..1925
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(1979..1998)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1980..1998)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2003..2019)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2003..2019)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2121..2140)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2132..2941
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2199..2218)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3088..3676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3577..3596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccccg gtggcggtgg atcgggagga ggaggttcgg
      601 gtggcggagg cagtggaggt ggcggaagtg gcggcggtgg ttcaggagga ggcggatcca
      661 tgagtaaagg agaagaattg ttcactggag ttgtcccaat cctcgtcgag ctcgacggag
      721 acgtcaacgg acacaagttc tccgtctccg gagagggaga gggagacgcc acctacggaa
      781 agctcaccct caagttcatc tgcaccaccg gaaagctccc agtcccatgg ccaaccctcg
      841 tcaccacctt ctgctacgga gtccaatgct tctcccgtta cccagaccac atgaagcgtc
      901 acgacttctt caagtccgcc atgccagagg gatacgtcca agagcgtacc atcttcttca
      961 aggtaagttt aaacatatat atactaacta ctgattattt aaattttcag gacgacggaa
     1021 actacaagac ccgtgccgag gtcaagttcg agggagacac cctcgtcaac cgtatcgagc
     1081 tcaaggtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagggaa
     1141 tcgacttcaa ggaggacgga aacatcctcg gacacaagct cgagtacaac tacaactccc
     1201 acaacgtcta catcatggcc gacaagcaaa agaacggaat caaggtcaac ttcaaggtaa
     1261 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagatc cgtcacaaca
     1321 tcgaggacgg atccgtccaa ctcgccgacc actaccaaca aaacacccca atcggagacg
     1381 gaccagtcct cctcccagac aaccactacc tctccaccca atccgccctc tccaaggacc
     1441 caaacgagaa gcgtgaccac atggtcctcc tcgagttcgt caccgccgcc ggaatcaccc
     1501 acggaatgga cgagctctac aagggcggca gcggaggaga gaatctgtac tttcaatccg
     1561 gatccggagg tggcggggga ttctccgaga ccgtcgacct catgctcaac ctccaatcca
     1621 acaaggaggg atccgtcgac ctcaagaacg tctccgccgt cccaaaggag aagaccaccc
     1681 tcaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggacc
     1741 catccaagcc accagccaag gcccaagtcg tcggatggcc accagtccgt aactaccgta
     1801 agaacatgat gacccaacaa aagacctcct ccggtggtgg cggttctggc tcaggatctg
     1861 actacaaaga ccatgacggt gattataaag atcatgatat cgattacaag gatgacgatg
     1921 acaagggagc atcgggagcc tcaggagcat cgtccggagg cgccatccag ctgatatccc
     1981 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2041 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     2101 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     2161 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     2221 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     2281 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2341 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2401 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     2461 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     2521 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     2581 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     2641 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     2701 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     2761 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     2821 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     2881 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     2941 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     3001 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3061 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3121 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3181 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3241 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3301 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3361 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3421 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3481 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3541 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3601 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3661 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3721 gccttttgct cacatgtt
//
