LOCUS       sequence-366532-       11046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-dependent expression of human cGAS gene in mammalian 
            cells by retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Mosallanejad K, Kennedy SN, Bahleda KM, Slavik KM, Zhou W, Govande 
            AA, Hancks DC, Kranzusch PJ, Kagan JC
  TITLE     Species-specific self-DNA detection mechanisms by mammalian cyclic 
            GMP-AMP synthases.
  JOURNAL   Sci Immunol. 2023 Jan 20;8(79):eabp9765. doi: 
            10.1126/sciimmunol.abp9765. Epub 2023 Jan 20.
  PUBMED    36662885
REFERENCE   2  (bases 1 to 11046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2023-01-20"; volume: "8(79):eabp9765. doi"; pages:
            " 10.1126/sciimmunol.abp9765. Epub 2023 Jan 20"
COMMENT     Sequence Label: pTetOne-human cGAS-HA
FEATURES             Location/Qualifiers
     source          1..11046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(281..1027)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(1046..1556)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1109..1127)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        1573..1940
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    1581..1599
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1617..1635
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1653..1671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1689..1707
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1725..1743
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1761..1779
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1797..1815
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3519..3545
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3729..3863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4011..4027)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(4365..4381)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4397..4419)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    4830..5187
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     enhancer        5618..5997
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(6172..6188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6172..6188)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6185..6207)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6196..6212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6220..6226,6227..6244,6245..6250))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6265..6286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6403..6420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6574..7162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6654..6673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7333..8124,8125..8193))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7956..7975
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8194..8298)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8366..8384
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8422..8444)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8544..8563
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8757..8779
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8771..8788
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8772..8788
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    8811..8932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    9952..10355
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10372..10971)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     10952..10971
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(join(10980..11046,1..263))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10994..11046,1..83)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
       61 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
      121 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
      181 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
      241 tttccacacc ctaactgaca cacattcaat tcttacttag ttacccgggg agcatgtcaa
      301 ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat
      361 cggctgggag catgtctaag tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc
      421 actttagctg tttctccagg ccacatatga ttagttccag gccgaaaagg aaggcaggtt
      481 cggctccctg ccggtcgaac agctcaattg cttgtttcag aagtgggggc atagaatcgg
      541 tggtaggtgt ctctctttcc tcttttgcta cttgatgctc ctgttcctcc aatacgcagc
      601 ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc gttctccagg gagaagcctt
      661 gctgacacag gaacgcgagc tgattttcca gggtttcgta ctgtttctct gttgggcggg
      721 tgccgagatg cactttagcc ccgtcgcgat gtgagaggag agcacagcgg tatgacttgg
      781 cgttgttccg cagaaagtct tgccatgact cgccttccag ggggcaggag tgggtatgat
      841 gcctgtccag catctcgatt ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc
      901 agtacagggt aggctgctca actcccagct tttgagcgag tttccttgtc gtcaggcctt
      961 cgataccgac tccattgagt aattccagag cagagtttat gactttgctc ttgtccagtc
     1021 tagacatggt aattcgatga tccccctggg gagagaggtc ggtgattcgg tcaacgaggg
     1081 agccgactgc cgacgtgcgc tccggaggct tgcagaatgc ggaacaccgc gcgggcagga
     1141 acagggccca cactaccgcc ccacaccccg cctcccgcac cgccccttcc cggccgctgc
     1201 tctcggcgcg ccctgctgag cagccgctat tggccacagc ccatcgcggt cggcgcgctg
     1261 ccattgctcc ctggcgctgt ccgtctgcga gggtactagt gagacgtgcg gcttccgttt
     1321 gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg acttaggggc ggagcaggaa
     1381 gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg ggtgacgctg cgaacggacg
     1441 tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt cccggaacca cgcccagagc
     1501 agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag gcgcaacccc aaccccgtgg
     1561 aattatcacc tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
     1621 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
     1681 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
     1741 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
     1801 tatcagtgat agagaacgta taagctttgc ttatgtaaac cagggcgcct ataaaagagt
     1861 gctgattttt tgagtaaact tcaattccac aacacttttg tcttatacca actttccgta
     1921 ccacttccta ccctcgtaaa gaattcgcca ccatgcagcc ttggcacgga aaggccatgc
     1981 agagagcttc cgaggccgga gccactgccc ccaaggcttc cgcacggaat gccaggggcg
     2041 ccccgatgga tcccaccgag tctccggctg cccccgaggc cgccctgcct aaggcgggaa
     2101 agttcggccc cgccaggaag tcgggatccc ggcagaaaaa gagcgccccg gacacccagg
     2161 agaggccgcc cgtccgcgca actggggccc gcgccaaaaa ggcccctcag cgcgcccagg
     2221 acacgcagcc gtctgacgcc accagcgccc ctggggcaga ggggctggag cctcctgcgg
     2281 ctcgggagcc ggctctttcc agggctggtt cttgccgcca gaggggcgcg cgctgctcca
     2341 cgaagccaag acctccgccc gggccctggg acgtgcccag ccccggcctg ccggtctcgg
     2401 cccccattct cgtacggagg gatgcggcgc ctggggcctc gaagctccgg gcggttttgg
     2461 agaagttgaa gctcagccgc gatgatatct ccacggcggc ggggatggtg aaaggggttg
     2521 tggaccacct gctgctcaga ctgaagtgcg actccgcgtt cagaggcgtc gggctgctga
     2581 acaccgggag ctactatgag cacgtgaaga tttctgcacc taatgaattt gatgtcatgt
     2641 ttaaactgga agtccccaga attcaactag aagaatattc caacactcgt gcatattact
     2701 ttgtgaaatt taaaagaaat ccgaaagaaa atcctctgag tcagttttta gaaggtgaaa
     2761 tattatcagc ttctaagatg ctgtcaaagt ttaggaaaat cattaaggaa gaaattaacg
     2821 acattaaaga tacagatgtc atcatgaaga ggaaaagagg agggagccct gctgtaacac
     2881 ttcttattag tgaaaaaata tctgtggata taaccctggc tttggaatca aaaagtagct
     2941 ggcctgctag cacccaagaa ggcctgcgca ttcaaaactg gctttcagca aaagttagga
     3001 agcaactacg actaaagcca ttttaccttg tacccaagca tgcaaaggaa ggaaatggtt
     3061 tccaagaaga aacatggcgg ctatccttct ctcacatcga aaaggaaatt ttgaacaatc
     3121 atggaaaatc taaaacgtgc tgtgaaaaca aagaagagaa atgttgcagg aaagattgtt
     3181 taaaactaat gaaatacctt ttagaacagc tgaaagaaag gtttaaagac aaaaaacatc
     3241 tggataaatt ctcttcttat catgtgaaaa ctgccttctt tcacgtatgt acccagaacc
     3301 ctcaagacag tcagtgggac cgcaaagacc tgggcctctg ctttgataac tgcgtgacat
     3361 actttcttca gtgcctcagg acagaaaaac ttgagaatta ttttattcct gaattcaatc
     3421 tattctctag caacttaatt gacaaaagaa gtaaagaatt tctgacaaag caaattgaat
     3481 atgaaagaaa caatgagttt ccagtttttg atgaatttta cccatacgat gttccagatt
     3541 acgcttgagg atccagacca cctcccctgc gagctaagct ggacagccaa tgacgggtaa
     3601 gagagtgaca tttttcacta acctaagaca ggagggccgt cagagctact gcctaatcca
     3661 aagacgggta aaagtgataa aaatgtatca ctccaaccta agacaggcgc agcttccgag
     3721 ggatttgaga tccagacatg ataagataca ttgatgagtt tggacaaacc aaaactagaa
     3781 tgcagtgaaa aaaatgcctt atttgtgaaa tttgtgatgc tattgcctta tttgtaacca
     3841 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3901 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     3961 attatgatct agaggatggt ccacccccgg ggtcggcagc cttcacgtgg gcggcgtgta
     4021 tccaagctgc gatgccgtct actttgaggg cggtgggggt ggtcagcagg actgtgtaag
     4081 gtcctttcca gcgaggttct aggttcttag tctggtgtcg gcggacccac actgtgtcgc
     4141 cgactcggta agggtgaggt accaccggtc ggtccagttg ttcttggtag gctgccgcca
     4201 gaggtctcca gacttcgtgc tggactaagt agagagcctg taagtgagct tggagagagg
     4261 ggctgttagt aactcttgtc atgtcagggt cagggaagtt tacaaggggc gggggtgccc
     4321 catataggat ctcatatggc catatggggg cgcctagaga aggagtgagg gctggataaa
     4381 gggaggatcg aggcggggtc gaacgaggag gttcaagggg gagagacggg gcggatggag
     4441 gaagaggagg cggaggctta gggtgtacaa agggcttgac ccagggaggg gggtcaaaag
     4501 ccaaggcttc ccaggtcacg atgtagggga cctggtctgg gtgtccatgc gggccaggtg
     4561 aaaagacctt gatcttaacc tgggtgatga ggtctcggtt aaaggtgccg tctcgcggcc
     4621 atccgacgtt aaaggttggc cattctgcag agcagaaggt aacccaacgt ctcttcttga
     4681 catctaccga ctggttgtga gcgatccgct cgacatcttt ccagtgacct aaggtcaaac
     4741 ttaagggagt ggtaacaggc tagcccgggc cctcattttc agacaaatac agaaacacag
     4801 tcagacagag acaacacaga acgatgctgc agcagacaag acgcgcggcg cggcttcggt
     4861 cccaaaccga aagcaaaaat tcagacggag gcgggaactg ttttaggttc tcgtctccta
     4921 ccagaaccac atatccctcc tctaaggggg gtgcaccaaa gagtcctaaa cgatcgggat
     4981 tttaggactc aggtcgggcc acaaaaacgg cccccgaagt ccctgggacg tctcccaggg
     5041 ttgcggccgg gtgttccgaa ctcgtcagtt ccaccacggg tccgccagat acagagctag
     5101 ttagctaact agtaccgacg caggcgcata aaatcagtca tagacactag acaatcggac
     5161 agacacagat aagttgctgg ccagcttacc tcccggtggt gggtcggtgg tccctgggca
     5221 ggggtctccc gatcccggac gagcccccaa atgaaagacc cccgctgacg ggtagtcaat
     5281 cactcagagg agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag
     5341 ggtttattgg atacacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag
     5401 tgaggggttg tgggctcttt tatagagctc ggggagcaga agcgctctag atgcccattt
     5461 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
     5521 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
     5581 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
     5641 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
     5701 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
     5761 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
     5821 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
     5881 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
     5941 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaac
     6001 atggcggtaa tgttggacat gagccaatat aaatgtacat attatgatat ggatacaacg
     6061 tatgcaatgg gccaagctcc tagggcatag aacaagtcaa atgctttata tatttgagtt
     6121 tgggatgtat taggaaactc ctaagagcaa agctagcttg gcgtaatcat ggtcatagct
     6181 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6241 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6301 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6361 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6421 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6481 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7321 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7381 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7441 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7501 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7561 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7621 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7681 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7741 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7801 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7861 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7921 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7981 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8041 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8101 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8161 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8221 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8281 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     8341 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     8401 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     8461 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     8521 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     8581 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
     8641 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     8701 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     8761 gtcacgacgt tgtaaaacga cggccagtga attaattcga cgcgccatga taagatacat
     8821 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     8881 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     8941 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     9001 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagagtcgg tgggcctcgg
     9061 gggcgggtgc ggggtcggcg gggccgcccc gggtggcttc ggtcggagcc atggggtcgt
     9121 gcgctccttt cggtcgggcg ctgcgggtcg tggggcgggc gtcaggcacc gggcttgcgg
     9181 gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac ggtgaagccg
     9241 agccgctcgt agaaggggag gttgcggggc gcggaggtct ccaggaaggc gggcaccccg
     9301 gcgcgctcgg ccgcctccac tccggggagc acgacggcgc tgcccagacc cttgccctgg
     9361 tggtcgggcg agacgccgac ggtggccagg aaccacgcgg gctccttggg ccggtgcggc
     9421 gccaggaggc cttccatctg ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg
     9481 cgcgggccga tctcggcgaa caccgccccc gcttcgacgc tctccggcgt ggtccagacc
     9541 gccaccgcgg tggcttaact atgcggcata cgcgtgaaag acccccgctg acgggtagtc
     9601 aatcactcag aggagaccct cccaaggaac agcgagacca caagtcggat gcaactgcaa
     9661 gagggtttat tggatacacg ggtacccggg cgactcagtc aatcggagga ctggcgcccc
     9721 gagtgagggg ttgtgggctc ttttgttgat gtctgggagc agaagcgcgc gaacagaagc
     9781 gagaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt ttctggagac
     9841 taaataaaat cttttatttt atctatggct cgtactctat aggcttcagc tggtgatatt
     9901 gttgagtcaa aactagagcc tggaccactg atatctccgc aggaagatct cgacgtcagc
     9961 ttccccgaca acaccacgga attatcagtg cccagcaacc tagcccctgt ccagcagcgg
    10021 gcaaggcagg cggcgatgag ttctgccgtg gcgataggga gggggaaagc gaaagtccca
    10081 gaaaggagtt gacaggtggt ggcaatgccc cagccagtgg gggttgcgtc agcaaacaca
    10141 gagcacacca cgccacgttg acggacaacg ggccacaact cctcataaag agacagcaac
    10201 caggatttat acaaggagga gaaaacgaaa gccgtacggg aagcaatagc atgatacaga
    10261 ggcattaaag cagcatatcc acacagcgta aaaggagcaa catagttaag aatatcagtc
    10321 aatctttcac aaattttgta atccagaggt tgattaacag gaattacgcg ttcaggcacc
    10381 gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac
    10441 ggtgaagccg agccgctcgt agaaggggag gttgcggggc gcggaggtct ccaggaaggc
    10501 gggcaccccg gcgcgctcgg ccgcctccac tccggggagc acgacggcgc tgcccagacc
    10561 cttgccctgg tggtcgggcg agacgccgac ggtggccagg aaccacgcgg gctccttggg
    10621 ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg gccagccggg aaccgctcaa
    10681 ctcggccatg cgcgggccga tctcggcgaa caccgccccc gcttcgacgc tctccggcgt
    10741 ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac accttgccga tgtcgagccc
    10801 gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc ggtccgggtc
    10861 gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc gtacggcccg
    10921 ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca tggtcgcgtt
    10981 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
    11041 gaggcg
//
