LOCUS       sequence-367194-        8766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.C.NWS_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(62..80)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     330..349
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    694..1282
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(747..767)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1165..1176)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1357..1590
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1662..1783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1699..1718)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1753..1772
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1823..1958
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1978..1997)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1979..1997)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2007..2024)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2007..2023)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2016..2038)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2165..2620
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2252..2271)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2462..2483
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2646..2750
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2751..2819,2820..3611)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2969..2988)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3782..4370
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4271..4290
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4524..4541
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4658..4679
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4694..4699,4700..4717,4718..4724)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4732..4748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4737..4759
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4756..4772
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4756..4772
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4791..4811
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4793..4811
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4839..5065
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5066..5246
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5293..5418
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5911..6144
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6329..6373
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6671..6919
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6671..6691
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6842..6861
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6938..7013
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    7061..7178
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7318..8496
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7549..8487
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8444..8464
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      8545..8554
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8593..8628
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             8650..8673
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             join(8683..8766,1..624)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
       61 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      121 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      181 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      241 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
      301 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      361 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
      421 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
      481 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
      541 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      601 ggcggcatgg acgagctgta caagaaacgg ccagccgagg atatggagga agaacaagcc
      661 tttaaacgat ctcgataaag cggccgcgtc gacaatcaac ctctggatta caaaatttgt
      721 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
      781 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
      841 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
      901 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
      961 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     1021 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     1081 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     1141 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     1201 ctgctgccgg ctctgcggcc tcttccgcat cttcgccttc gccctcagac gagtcggatc
     1261 tccctttggg ccgcctcccc gcctgggtac ctttaagacc aatgacttac aaggcagctg
     1321 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     1381 gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     1441 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     1501 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     1561 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     1621 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     1681 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     1741 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     1801 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     1861 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     1921 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     1981 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     2041 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2101 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2161 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2221 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2281 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2341 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     2401 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2461 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     2521 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2581 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     2641 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2701 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2761 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2821 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2881 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2941 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3001 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3061 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3121 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3181 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3241 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3301 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3361 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3421 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3481 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3541 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3601 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3661 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3721 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3781 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3841 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3901 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3961 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4021 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4081 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4141 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4201 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4261 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4321 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4381 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4441 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4501 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4561 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4621 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     4681 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     4741 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     4801 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     4861 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     4921 ccgtgcatgg cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     4981 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     5041 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     5101 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5161 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5221 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     5281 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     5341 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     5401 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     5461 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     5521 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     5581 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     5641 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     5701 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     5761 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     5821 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     5881 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     5941 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     6001 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     6061 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     6121 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     6181 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     6241 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     6301 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     6361 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     6421 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     6481 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     6541 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     6601 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagtatc gagcggccgc
     6661 ccccttcacc gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
     6721 tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
     6781 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
     6841 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
     6901 gtggaaagga cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag
     6961 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
     7021 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     7081 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     7141 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     7201 gagatccact ttggccgcgg ctcgaggcga taagctttgc aaagatggat aaagttttaa
     7261 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc
     7321 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     7381 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     7441 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     7501 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     7561 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     7621 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     7681 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     7741 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     7801 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     7861 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     7921 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     7981 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     8041 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     8101 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     8161 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     8221 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     8281 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     8341 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     8401 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     8461 gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa ttctgcagtc gatcgacggt
     8521 accgcgggcc cgggatccac ggtcgccacc atgggcgaca cctatcggta cattggagga
     8581 ggcgggggag gggaggacca ggtggacccc aggctgatcg acggcaaggg aggaggcggg
     8641 ggagggaact ggtcccaccc ccaattcgag aaaggcatgc tgatggtgag caagggcgag
     8701 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     8761 gtgaac
//
