LOCUS       sequence-367234-        1321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Cas12b from A. acidoterrestris (type V-B).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Wu WY, Jackson SA, Almendros C, Haagsma AC, Yilmaz S, Gort G, van 
            der Oost J, Brouns SJJ, Staals RHJ
  TITLE     Adaptation by Type V-A and V-B CRISPR-Cas Systems Demonstrates 
            Conserved Protospacer Selection Mechanisms Between Diverse 
            CRISPR-Cas Types.
  JOURNAL   CRISPR J. 2022 Aug;5(4):536-547. doi: 10.1089/crispr.2021.0150. Epub
            2022 Jul 12.
  PUBMED    35833800
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2021.0150"; journalName: "CRISPR J"; date: 
            "2022-08"; volume: "5"; issue: "4"; pages: "536-547"
COMMENT     Sequence Label: pCas12b
FEATURES             Location/Qualifiers
     source          1..1321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1045..1064
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1045..1063
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1198..1242
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(1280..1298)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attatggagg ctctcggcta tgtgtacgcg ttggatgagc gcggcaaagg aaagtgggtt
       61 gcgaagtatc cgccgtgcca gctcatcctg ctggaggaat tgagcgagta ccagttcaat
      121 aacgacaggc ctccgagcga aaacaaccag ttgatgcaat ggagtcatcg cggcgtgttc
      181 caggagttga taaatcaggc ccaagtccat gatttactcg ttgggacgat gtatgcagcg
      241 ttctcgtcgc gattcgacgc gcgaactggg gcaccgggta tccgctgtcg ccgggttccg
      301 gcgcgttgca cccaggagca caatccagaa ccatttcctt ggtggctgaa caagtttgtg
      361 gtggaacata cgttggatgc ttgtccccta cgcgcagacg acctcatccc aacgggtgaa
      421 ggagagattt twswstcgcc gttcagcgcg gaggaggggg actttcatca gattcacgcc
      481 gacctgaatg cggcgcaaaa tctgcagcag cgactctggt ctgattttga tatcagtcaa
      541 attcggttgc ggtgtgattg gggtgaagtg gacggtgaac tcgttctgat cccaaggctt
      601 acaggaaaac gaacggcgga ttcatatagc aacaaggtgt tttataccaa tacaggtgtc
      661 acctattatg agcgagagcg ggggaagaag cggagaaagg ttttcgcgca agagaaattg
      721 tcggaggaag aggcggagtt gctcgtggaa gcagacgagg cgagggagaa atcggtcgtt
      781 ttgatgcgtg atccgtctgg catcatcaat cggggaaatt ggaccaggca aaaggaattt
      841 tggtcgatgg tgaaccagcg gatcgaagga tacttggtca agcagattcg ctcgcgcgtt
      901 ccattacaag atagtgcgtg tgaaaacacg ggggatattt aagaattcga gctcggcgcg
      961 cctgcaggtc gacaagcttg cggccgcata atgcttaagt cgaacagaaa gtaatcgtat
     1021 tgtacacggc cgcataatcg aaattaatac gactcactat aggggaattg ntgwsysgat
     1081 aacaattccc catcttagta tattagttaa gtataagaag gagatataca tatggcagat
     1141 ctcaattgga tatcggccgg ccacgcgatc gctgacgtcg gtaccctcga gtctggtaaa
     1201 gaaaccgctg ctgcgaaatt tgaacgccag cacatggact cgtctactag cgcagcttaa
     1261 ttaacctagg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     1321 c
//
