LOCUS       sequence-36732-e        5981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Kaps I, Ehrt S, Seeber S, Schnappinger D, Martin C, Riley LW, 
            Niederweis M
  TITLE     Energy transfer between fluorescent proteins using a co-expression 
            system in Mycobacterium smegmatis.
  JOURNAL   Gene. 2001 Oct 31;278(1-2):115-24.
  PUBMED    11707328
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2001-10-31"; volume: "278(1-2)"; pages: "115-24"
COMMENT     Sequence Label: pMycVec1
FEATURES             Location/Qualifiers
     source          1..5981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     515..531
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     516..532
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(663..690)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      819..1407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1308..1327
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1561..1578
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1593..1733
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1659..1678)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1819..1841
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1835..2026)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             2873..3667
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2927..2946)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3537..3556
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tgccgcctga atgcgcgggt acgagccaca cagcgcccga acttacggag ctggtgggct
       61 cacctggctt tatggcgtac gaatcggctg tgtgcgacct gttgggcgag gtgaggtacg
      121 cgctactcac gctggcaagg gcgacacagc cgccccaccg agcccgcacg gccgcgcgcg
      181 gtgtcaacaa ccgggtgagt cgtgcacacc agcaggtgtt cgaggcttgg ctcgaagtgc
      241 aggacatcgt ggcgaacgcc gcccgatgag ccgcgcctta cgctggctgc cagccgttcg
      301 cgggctggtt ggtgcagcgc gtcgagcggt tagaggccct gcggtgttcc accaccgcag
      361 gcctcgccct ttttaaggct gaatttgctt gtctccgaat ccaactggct tgtccaaggg
      421 tgtatctacg cttaatccaa agttcaaacg aggggattac acatgaccaa cttcgataac
      481 gttctcggct cgatgatccc cggcgggccc cccctcgagg tcgacggtat cgataaaata
      541 aaaaagggga cctctagggt ccccaattaa ttagctaaag cttgatatcg aattcctgca
      601 gcccggggga tccatttaaa tcagctgtta attaatctag agtttaaaca gtattaaacg
      661 caaaaaggcc atccgtcagg atggccttct gcttagctaa tcaactagaa aaggatctag
      721 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
      781 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      841 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      901 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      961 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1021 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1081 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1141 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1201 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1261 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1321 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1381 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1441 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1501 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1561 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     1621 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     1681 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     1741 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     1801 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     1861 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt
     1921 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
     1981 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
     2041 aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga
     2101 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg
     2161 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta
     2221 atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca
     2281 taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga
     2341 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct
     2401 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc
     2461 tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgacgact
     2521 tcacgctgcc gcaagcactc agggcgcaag ggctgctaaa aggaagcgga acacgtagaa
     2581 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
     2641 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
     2701 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
     2761 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
     2821 atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     2881 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     2941 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     3001 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     3061 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     3121 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     3181 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     3241 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     3301 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     3361 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     3421 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     3481 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     3541 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     3601 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     3661 cttctgagcg ggactctggg tacctatggc cgcccggagc cccgcccgcg ctcgggagcc
     3721 ccgggcccgc gccgaagccc gctgctgcga gcccggagcg ggccggccga cggcggtgcg
     3781 ggcccggcgg cggacgctca gcagcggcgg gcgtgaaagg ccctggcatc ctcgatcatc
     3841 tcctccaggg tggtcggccg agcttccatc ccagctcggc aaggatcaac gaggacagtc
     3901 gcacgacgaa gttcttctgg atcgcgcccg tgctggaagc actcaacctc gaagcgtgtg
     3961 gttgcggagc catctagcaa ccacacgaaa catgcgcaac gaaccgcgca acgaacaacg
     4021 cctagaactg gccctagatg agctgactcg tatcgttggt aaacctagtt tgaccagcat
     4081 gttttaacta cgttcggtga gctgtcaacg gggcctgtaa cggcacaacg aaccgtgcaa
     4141 cgagagtggc cacggatgcc accacaggca ctacaacgga gttcgccacg tacatcacca
     4201 caaccaccga ttctggcggt gagctccccg atattcagcg gaaatggctt ggtatcgacc
     4261 aagattcgta gaaccccgtc tcgtctggct ggtattcaaa acgggcgcaa cgaaacacgc
     4321 aacgagacag gcatggccca aaccagaaaa ctagcgtcta ccaggacttt tacctgtccg
     4381 acccgttgca acggaacccc ccacggaacc cccgcgacac ccgctcccca attgcgttag
     4441 aacagcggtg gattgtcggc ttcgttgtgg gccttttgag ccgcttcctg ttctgccgca
     4501 cgctctttcc tcgcccgata gccgagtcgc ttaacggtgt ccagatgcag cccgaaatgt
     4561 ttggccgttt gcggccaaga gtggccctcg tcgtcgtgat aggcgcggat gcgttcgcgg
     4621 cgtgcagcct gctcggcgag ccactcgctg cgttcctgcg ccacgagccg gacgacgtgg
     4681 cgttcggata gtccggtgat tcgagcgcct tcggcggcgg tcacgcgccg ctttttgcgg
     4741 acagtcggct gccggttgta gccgtcgctg tagccgtcgc tgtagccgtc gctcatagca
     4801 atgcctccat ggctgacgcg gactttgcgc gccgcgcaac tgtgctcgcc gccgtgcgcg
     4861 ctgctgcgcc cttccgcgag atggccgact ggcgcgcact gagtgtggcc tcgtagacca
     4921 cgatcccgtc cgcccaaatg cgcgacttgg ttgtgatcca acgccaaatg ctgttggcga
     4981 tggcgcggac ctcgctgtcc ggtagcggtc cgggacacac gtcgttgcac gggaattcgg
     5041 cgtttcgcgc gtggcactcg gcatagatcg cgcggccgag tccgtccacg ttccgggtcg
     5101 gcaggtagat ccgcatgagg gcgggacgat aggcccacaa cctgacggaa tcgaacagtg
     5161 cgcaattccg ccctagcggc gtcggagccg ctttgtacgt ggtctgctga cgccagcgcg
     5221 gcggtggcat gttcgcgccg agctcggcct cgatgtggct gagtgtgtag agatctgagt
     5281 ggagccattc cgtttcccag gcgatgtggc cggggttttt ggtcatgagg cctgagtaac
     5341 tgcggtcgcc gtcgacggcg cgccgaaggc cttcggcgca cgccgccatg tatgcgagcg
     5401 gcttacgccg cgcgtattcg gtgcgtggaa caggggcgtt gagtgcccac actgcgtgtg
     5461 cgtggccgtt ggcgcgattg cccacgatcg cgttgggcag cggatgggac ccccgggcgc
     5521 tgagcgctcg gagcgctgcg tctggatggt ctacgtccac gaccagcagg tttgccagcg
     5581 ctgttgggtt cgcctcgatg taccggcggc ctagggccga cgcgcggctt tggcggtaga
     5641 tcccctcgag cagatcgtcg cttgccagcg gccagtacgg cagccagagc tgctcaaatt
     5701 cgtcggcgac gtggctcacg cttggtagta gaccacgatt aatcaccggt gtatggtccg
     5761 acacgagctc caagtcagat atttcgctga ggggccaccc cacaactgca cactcccccg
     5821 ctctcccgtc gagccctggt ggtggaacac cagcgacagc cgagcacccc caaccacctg
     5881 taccaaccag gaggaacaca tgcgtcgttt cgaggacgtt tccgggccgc tgagagccgc
     5941 tgtggcggcc gtacacgccg ccttagaccc gttagacccc c
//
