LOCUS       sequence-367355-       10592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  baculovirus protein expression of mouse MD-2 (residues 19–160) fused
            to a protein A tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Wang Y, Su L, Morin MD, Jones BT, Whitby LR, Surakattula MM, Huang 
            H, Shi H, Choi JH, Wang KW, Moresco EM, Berger M, Zhan X, Zhang H, 
            Boger DL, Beutler B
  TITLE     TLR4/MD-2 activation by a synthetic agonist with no similarity to 
            LPS.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):E884-93. doi: 
            10.1073/pnas.1525639113. Epub 2016 Feb 1.
  PUBMED    26831104
REFERENCE   2  (bases 1 to 10592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1525639113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-02-16- 16"; volume: "113"; issue: "7"; pages: "E884-93"
COMMENT     Sequence Label: pAcGP67A-mMD2-proA
FEATURES             Location/Qualifiers
     source          1..10592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             3170..3294
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(3332..3937)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        4001..4092
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4017..4034
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     4189..4248
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             4690..4710
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4744..4917
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             4918..5091
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     misc_recomb     5600..6305
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           5688..6060
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(5717..6305)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(6739..6756)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7973..7989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7973..7989)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7986..8008)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7997..8013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8021..8027,8028..8045,8046..8051))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8066..8087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8204..8221)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8375..8963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8455..8474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9134..9925,9926..9994))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9757..9776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9995..10099)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10167..10185
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10223..10245)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10345..10364
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10558..10580
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10572..10589
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10573..10589
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttggct cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatat gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgtcaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atctatgcta
     4141 ctagtaaatc agtcacacca aggcttcaat aaggaacaca caagcaagat ggtaagcgct
     4201 attgttttat atgtgctttt ggcggcggcg gcgcattctg cctttgcggc ggatcccgag
     4261 aagcaacagt ggttctgcaa ctcctccgat gcaattattt cctacagtta ttgtgatcac
     4321 ttgaaattcc ctatttcaat tagttctgaa ccctgcataa gactgagggg aaccaatgga
     4381 tttgtgcatg ttgagttcat tccaagagga aacttaaaat atttatattt caacctattc
     4441 atcagtgtca actccataga gttgccgaag cgtaaggaag ttctgtgcca tggacatgat
     4501 gatgactatt ctttttgcag agctctgaaa ggagagactg tgaatacatc aataccattc
     4561 tctttcgagg gaatactatt tcctaagggc cattacagat gtgttgcaga agctattgct
     4621 ggggatactg aagaaaagct cttctgtttg aatttcacca tcattcaccg ccgtgatgtc
     4681 aataagggcg aaaacttgta ctttcaaggc gaattcgcag gccttgcgca acacgatgaa
     4741 gccgtggaca acaaattcaa caaagaacaa caaaacgcgt tctatgagat cttacattta
     4801 cctaacttaa acgaagaaca acgaaacgcc ttcatccaaa gtttaaaaga tgacccaagc
     4861 caaagcgcta accttttagc agaagctaaa aagctaaatg atgctcaggc gccgaaagta
     4921 gacaacaaat tcaacaaaga acaacaaaac gcgttctatg agatcttaca tttacctaac
     4981 ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc
     5041 gctaaccttt tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacgcg
     5101 tgagcggccg ctgcagatct gatcctttcc tgggacccgg caagaaccaa aaactcactc
     5161 tcttcaagga aatccgtaat gttaaacccg acacgatgaa gcttgtcgtt ggatggaaag
     5221 gaaaagagtt ctacagggaa acttggaccc gcttcatgga agacagcttc cccattgtta
     5281 acgaccaaga agtgatggat gttttccttg ttgtcaacat gcgtcccact agacccaacc
     5341 gttgttacaa attcctggcc caacacgctc tgcgttgcga ccccgactat gtacctcatg
     5401 acgtgattag gatcgtcgag ccttcatggg tgggcagcaa caacgagtac cgcatcagcc
     5461 tggctaagaa gggcggcggc tgcccaataa tgaaccttca ctctgagtac accaactcgt
     5521 tcgaacagtt catcgatcgt gtcatctggg agaacttcta caagcccatc gtttacatcg
     5581 gtaccgactc tgctgaagag gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa
     5641 aggagtttgc accagacgca cctctgttca ctggtccggc gtattaaaac acgatacatt
     5701 gttattagta catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt
     5761 gtacgtattt taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa
     5821 tcaaatgatt ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt
     5881 aaaataggtt tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat
     5941 ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt
     6001 tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat
     6061 gcgcttttgt atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt
     6121 taaataaagc ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt
     6181 cgtcgtccca accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac
     6241 gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg
     6301 gaattatttc tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta
     6361 attcagacaa cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat
     6421 ctactaatgg cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg
     6481 gggctggcgg cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct
     6541 caaatgtctc tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgccg
     6601 tttttggttt gaccggtctg agacgagtgc gatttttttc gtttctaata gcttccaaca
     6661 attgttgtct gtcgtctaaa ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg
     6721 gcggcaattc agacatcgat ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg
     6781 gagaaggtgg tggcggcggt gccgccggta taatttgttc tggtttagtt tgttcgcgca
     6841 cgattgtggg caccggcgca ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag
     6901 gcagcgcttg gggtggtggc aattcaatat tataattgga atacaaatcg taaaaatctg
     6961 ctataagcat tgtaatttcg ctatcgttta ccgtgccgat atttaacaac cgctcaatgt
     7021 aagcaattgt attgtaaaga gattgtctca agctcgcacg ccgataacaa gccttttcat
     7081 ttttactaca gcattgtagt ggcgagacac ttcgctgtcg tcgacgtaca tgtatgcttt
     7141 gttgtcaaaa acgtcgttgg caagctttaa aatatttaaa agaacatctc tgttcagcac
     7201 cactgtgttg tcgtaaatgt tgtttttgat aatttgcgct tccgcagtat cgacacgttc
     7261 aaaaaattga tgcgcatcaa ttttgttgtt cctattattg aataaataag attgtacaga
     7321 ttcatatcta cgattcgtca tggccaccac aaatgctacg ctgcaaacgc tggtacaatt
     7381 ttacgaaaac tgcaaaaacg tcaaaactcg gtataaaata atcaacgggc gctttggcaa
     7441 aatatctatt ttatcgcaca agcccactag caaattgtat ttgcagaaaa caatttcggc
     7501 gcacaatttt aacgctgacg aaataaaagt tcaccagtta atgagcgacc acccaaattt
     7561 tataaaaatc tattttaatc acggttccat caacaaccaa gtgatcgtga tggactacat
     7621 tgactgtccc gatttatttg aaacactaca aattaaaggc gagctttcgt accaacttgt
     7681 tagcaatatt attagacagc tgtgtgaagc gctcaacgat ttgcacaagc acaatttcat
     7741 acacaacgac ataaaactcg aaaatgtctt atatttcgaa gcacttgatc gcgtgtatgt
     7801 ttgcgattac ggattgtgca aacacgaaaa ctcacttagc gtgcacgacg gcacgttgga
     7861 gtattttagt ccggaaaaaa ttcgacacca caactatgca cgttcgtttg actggtacgc
     7921 cgtcggcgtg ttaacataca agttgctaac cggcggccaa ttcgtaatca tggtcatagc
     7981 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8041 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8101 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8161 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8221 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8281 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8341 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8401 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8461 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8521 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8581 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8641 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8701 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8761 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8821 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8881 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8941 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9001 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9061 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9121 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9181 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9241 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9301 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9361 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9421 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9481 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9541 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9601 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9661 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9721 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9781 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9841 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9901 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9961 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10021 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10081 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10141 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
    10201 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10261 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10321 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
    10381 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
    10441 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    10501 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    10561 agtcacgacg ttgtaaaacg acggccagtg cc
//
