LOCUS       sequence-367366-        4350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF768 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF768
FEATURES             Location/Qualifiers
     source          1..4350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1661..1760)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1777..1796)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1778..1796)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1801..1817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1801..1817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2248..3039
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3132..3720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3621..3640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3874..3891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4050..4077)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4169..4255)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4304..4326
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4318..4335
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4319..4335
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgaggagccc cgaagggtac
      121 ctcagaggca acatgagtga gaatgaggaa gaggaaattt ctcagcaaga aggcagtggg
      181 gactatgaag tcgaagagat accatttggg cttgaacccc agagccctgg gtttgagcca
      241 caaagcccag agtttgaacc ccaaagcccc agatttgagc ctgaaagccc ggggtttgag
      301 tcccgaagcc ctgggcttgt gcccccaagc cctgagtttg cacccagaag ccctgaatca
      361 gattctcaga gccctgagtt tgaatcccag agccctaggt atgaacccca aagccctggc
      421 tatgaacctc ggagccccgg gtatgaaccc cggagccctg gctatgaatc tgagagctct
      481 agatatgaat cccagaacac tgagctcaaa acccaaagcc cagaatttga agctcaaagt
      541 tccaaattcc aggaaggtgc ggagatgctt ctgaaccccg acgaaaagag tcctttgaat
      601 atctccgtag gagttcaccc cctggactcc ttcactcagg ggtttgggga gcagcccaca
      661 ggggacctgc ccatagggcc accttttgag atgcccacag gggccctgct gtctacaccg
      721 cagtttgaga tgcttcagaa tcccctgggt ctcacaggag cccttcgagg tccaggtcgg
      781 cggggtggcc gggccagggg tgggcagggc cctcggccta acatctgcgg catctgcggg
      841 aagagcttcg ggcggggctc caccctgatc cagcaccagc gcatccacac cggtgagaag
      901 ccctacaaat gtgaggtctg cagcaaggcc ttctcccaga gctctgacct catcaaacac
      961 cagcgcaccc acactggcga gcggccctac aaatgtcccc gttgcggcaa ggccttcgcc
     1021 gacagctctt acctgcttcg ccaccagcgc actcactctg gccagaagcc ctacaagtgc
     1081 ccacattgtg gcaaggcctt cggcgacagc tcctacctcc tgcgacacca gcgcacccac
     1141 agccacgagc ggccctacag ctgcaccgag tgcggcaagt gctatagcca gaactcgtcc
     1201 ctgcgcagcc atcagagggt gcacaccggt cagaggccct tcagctgtgg catctgcggc
     1261 aagagcttct cccagcggtc ggcccttatc ccccatgccc gcagccacgc ccgggagaag
     1321 cccttcaagt gccctgagtg cggcaagcgc tttggccaga gctcggtgct ggccatccac
     1381 gcccgcaccc acctgccagg ccgcacctac agctgccccg actgcggcaa gaccttcaat
     1441 cgctcctcca ctctcatcca gcaccagcgc tcccacacgg gcgagcggcc ctacaggtgc
     1501 gccgtgtgcg gcaagggctt ctgccgctcc tccacgcttc tgcagcatca ccgggtccac
     1561 agtggcgagc ggccttacaa gtgcgatgac tgcggaaagg ccttctccca gagctccgac
     1621 ctcatccgcc accagcggac ccacgcggcg ggccggcgct gcccaacttt cttgtacaaa
     1681 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1741 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1801 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1861 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     1921 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     1981 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     2041 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     2101 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     2161 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     2221 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact
     2281 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     2341 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     2401 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     2461 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     2521 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2581 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2641 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2701 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2761 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2821 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2881 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2941 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     3001 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     3061 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     3121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3241 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3841 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3901 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3961 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     4021 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     4081 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     4141 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     4201 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     4261 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     4321 aaaacgacgg ccagtcttaa gctcgggccc
//
