LOCUS       sequence-367402-        9950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shApc-expressing lentiviruses with varius levels of expression of 
            SIINFEKL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Westcott PMK, Sacks NJ, Schenkel JM, Ely ZA, Smith O, Hauck H, 
            Jaeger AM, Zhang D, Backlund CM, Beytagh MC, Patten JJ, Elbashir R, 
            Eng G, Irvine DJ, Yilmaz OH, Jacks T
  TITLE     Low neoantigen expression and poor T-cell priming underlie early 
            immune escape in colorectal cancer.
  JOURNAL   Nat Cancer. 2021 Oct;2(10):1071-1085. doi: 
            10.1038/s43018-021-00247-z. Epub 2021 Sep 30.
  PUBMED    34738089
REFERENCE   2  (bases 1 to 9950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s43018-021-00247-z"; journalName: "Nat Cancer"; date: 
            "2021-10"; volume: "2"; issue: "10"; pages: "1071-1085"
COMMENT     Sequence Label: hiSIIN
FEATURES             Location/Qualifiers
     source          1..9950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      167..302
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     229..248
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        364..411
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             430..804
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    934..1055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(971..990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1025..1044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1104..1120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1104..1120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1117..1139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1128..1144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1152..1158,1159..1176,1177..1182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1197..1218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1335..1352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1506..2094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1586..1605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2265..3056,3057..3125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2888..2907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3126..3230)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3305..3324)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3496..3875
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3877..4075
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4030..4050
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4093..4273
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4320..4445
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4938..5171
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5356..5400
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5698..5815
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5896..7074
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6127..7065
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7022..7042
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7105..7800
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(7238..7256)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7506..7525
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7813..7836
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
     ncRNA           7883..8004
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           8032..8046
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           8074..8200
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    8300..8888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8353..8373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8771..8782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8960..9193
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9219..9236)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9225..9449
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9495..9923
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9582..9601)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9792..9813
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(join(9932..9950,1..2))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(9937..9950,1..316)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
       61 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      121 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
      181 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
      241 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
      301 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
      361 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
      421 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
      481 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
      541 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
      601 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
      661 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
      721 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
      781 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
      841 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
      901 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
      961 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1021 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     1081 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1141 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     1201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     1261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     1321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1441 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1921 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2281 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2341 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2401 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2461 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2521 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2581 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2641 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2701 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2761 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2821 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2881 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2941 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3001 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3061 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3121 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3181 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3241 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     3301 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     3361 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     3421 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     3481 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     3541 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     3601 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     3661 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     3721 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     3781 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     3841 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     3901 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     3961 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     4021 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
     4081 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4141 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4201 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4261 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     4321 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     4381 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     4441 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     4501 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     4561 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     4621 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     4681 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     4741 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     4801 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     4861 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     4921 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     4981 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     5041 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     5101 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     5161 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     5221 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     5281 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     5341 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     5401 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     5461 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     5521 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     5581 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     5641 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     5701 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     5761 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     5821 ttattacagg gacagcagag atccagtttg gttaattaag gtaccgatca gtgtgaggga
     5881 gtgtaaagct ggtttggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     5941 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     6001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     6061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     6121 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     6181 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     6241 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     6301 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     6361 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     6421 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     6481 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     6541 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     6601 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     6661 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     6721 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     6781 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     6841 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     6901 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     6961 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     7021 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgactaact
     7081 cgaacgctag ctgtgcgatc gtttatggtg agcaagggcg aggcagtgat caaggagttc
     7141 atgcggttca aggtgcacat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     7201 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     7261 ggccccctgc ccttctcctg ggacatcctg tcccctcagt tcatgtacgg ctccagggcc
     7321 ttcaccaagc accccgccga catccccgac tactataagc agtccttccc cgagggcttc
     7381 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc
     7441 tccctggagg acggcaccct gatctacaag gtgaagctcc gcggcaccaa cttccctcct
     7501 gacggccccg taatgcagaa gaagacaatg ggctgggaag cgtccaccga gcggttgtac
     7561 cccgaggacg gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa ggacggcggc
     7621 cgctacctgg cggacttcaa gaccacctac aaggccaaga agcccgtgca gatgcccggc
     7681 gcctacaacg tcgaccgcaa gttggacatc acctcccaca acgaggacta caccgtggtg
     7741 gaacagtacg aacgctccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     7801 gagcagctcg agagtataat caactttgaa aaactgtaat gacgtacggc taagcctcga
     7861 ctagggataa cagggtaatt gtttgaatga ggcttcagta ctttacagaa tcgttgcctg
     7921 cacatcttgg aaacacttgc tgggattact tcttcaggtt aacccaacag aaggctcgag
     7981 aaggtatatt gctgttgaca gtgagcgcca ccaataaatt acagtcttat agtgaagcca
     8041 cagatgtata agactgtaat ttattggtgt tgcctactgc ctcggaattc aaggggctac
     8101 tttaggagca attatcttgt ttactaaaac tgaatacctt gctatctctt tgatacattt
     8161 ttacaaagct gaattaaaat ggtataaatt aaatcacttt tttcaattct accaaacgtt
     8221 gtttgtttgg ggttgaatta ctctacatgt ttaagggttc cggttccact aggtacaatt
     8281 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     8341 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     8401 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     8461 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     8521 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     8581 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     8641 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     8701 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8761 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8821 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     8881 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8941 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     9001 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9061 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9121 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9181 aaaatctcta gcagggcccg gcgaaacccg ctgatcagcc tcgactgtgc cttctagttg
     9241 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     9301 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     9361 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     9421 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     9481 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     9541 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     9601 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     9661 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     9721 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     9781 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     9841 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     9901 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
//
