LOCUS       sequence-367717-         579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human TTR in E coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Sun X, Ferguson JA, Dyson HJ, Wright PE
  TITLE     A transthyretin monomer intermediate undergoes local unfolding and 
            transient interaction with oligomers in a kinetically concerted 
            aggregation pathway.
  JOURNAL   J Biol Chem. 2022 Jun 18;298(8):102162. doi: 
            10.1016/j.jbc.2022.102162.
  PUBMED    35724960
REFERENCE   2  (bases 1 to 579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-06-18"; volume: "298(8):102162. doi"; pages: " 
            10.1016/j.jbc.2022.102162"
COMMENT     Sequence Label: TTR C10S S46C
FEATURES             Location/Qualifiers
     source          1..579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(476..494)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      490..537
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctagaataat tttgtttaac tttaagaagg agatatacat atgggtccta cgggcaccgg
       61 tgaatccaag agtcctctga tggtcaaagt tctagatgct gtccgaggca gtcctgccat
      121 caatgtggcc gtgcatgtgt tcagaaaggc tgctgatgac acctgggagc catttgcctg
      181 tgggaaaacc agtgagtctg gagagctgca tgggctcaca actgaggagg aatttgtaga
      241 agggatatac aaagtggaaa tagacaccaa atcttactgg aaggcacttg gcatctcccc
      301 attccatgag catgcagagg tggtattcac agccaacgac tccggccccc gccgctacac
      361 cattgccgcc ctgctgagcc cctactccta ttccaccacg gctgtcgtca ccaatcccaa
      421 ggaatgagat ccggctgcta caaagcccga aaggaagctg agttggctgc tgccaccgct
      481 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      541 aaaggaggaa ctatatccgg attggcgaat gggacgcgc
//
