LOCUS       sequence-367821-        7883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of Parkin tagged with BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, 
            Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn 
            M, Lindblom RSJ, Martens S, Lazarou M
  TITLE     Unconventional initiation of PINK1/Parkin mitophagy by Optineurin.
  JOURNAL   Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 
            10.1016/j.molcel.2023.04.021.
  PUBMED    37207627
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.04"; journalName: "Mol Cell"; date: 
            "2023-05-18- 18"; volume: "83"; issue: "10"; pages: "1693-1709.e9"
COMMENT     Sequence Label: pBMN BFP-Parkin
FEATURES             Location/Qualifiers
     source          1..7883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(134..238)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  383..1150
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     LTR             1552..2021
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2086..2443
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2508..2924
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2854..2876
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2892..2908
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    2934..3308
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     3246..3262
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             3317..4015
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             5634..6103
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6233..6250)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6404..6992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6484..6503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7156..7883,1..64,65..133))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7779..7798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
       61 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
      121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
      241 catttccccg aaaagtgcca ccagctttgc tcttaggagt ttcctaatac atcccaaact
      301 caaatatata aagcatttga cttgttctat gccctagggg gcggggggaa gctaagccag
      361 ctttttttaa catttaaaat gtggtaatga ctccaactta ttgatagtgt tttatgttca
      421 gataatgccc gatgactttg tcatgcagct ccaccgattt tgagaacgac agcgacttcc
      481 gtcccagccg tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat
      541 atcgcttgct gattacgtgc agctttccct tcaggcggga ttcatacagc ggccagccat
      601 ccgtcatcca tatcaccacg tcaaagggtg acagcaggct cataagacgc cccagcgtcg
      661 ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt ccggagactg tcatacgcgt
      721 aaaacagcca gcgctggcgc gatttagccc cgacatagcc ccactgttcg tccatttccg
      781 cgcagacgat gacgtcactg cccggctgta tgcgcgaggt taccgactgc ggcctgagtt
      841 ttttaagtga cgtaaaatcg tgttgaggcc aacgcccata atgcgggctg ttgcccggca
      901 tccaacgcca ttcatggcca tatcaatgat tttctggtgc gtaccgggtt gagaagcggt
      961 gtaagtgaac tgcagttgcc atgttttacg gcagtgagag cagagatagc gctgatgtcc
     1021 ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaacaggagg gacagctgat
     1081 agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc agtaagttgg
     1141 cagcatcacc ttaaaatgtt aattccattt taaatgcaca gatgttttta tttcataagg
     1201 gtttcaatgt gcatgaatgc tgcaatattc ctgttaccaa agctagtata aataaaaata
     1261 gataaacgtg gaaattactt agagtttctg tcattaacgt ttccttcctc agttgacaac
     1321 ataaatgcgc tgctgagaag ccagtttgca tctgtcagga tcaatttccc attatgccag
     1381 tcatattaat tactagtcaa ttagttgatt tttatttttg acatatacat gtgaaagacc
     1441 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
     1501 cataactgag aatagaaaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
     1561 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     1621 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     1681 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
     1741 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     1801 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     1861 cacaacccct cactcggcgc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     1921 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     1981 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
     2041 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
     2101 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta
     2161 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttcggaac
     2221 acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt gtggcccgac
     2281 ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctta gaggagggat
     2341 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
     2401 tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt
     2461 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatat gggcccgggc cagactgtta
     2521 ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc
     2581 agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct
     2641 ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga
     2701 tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct
     2761 gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc
     2821 cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc
     2881 ctcgatcctc cctttatcca gccctcactc cttctctagg cgcccccata tggccatatg
     2941 agatcttata tggggcaccc ccgccccttg taaacttccc tgaccctgac atgacaagag
     3001 ttactaacag cccctctctc caagctcact tacaggctct ctacttagtc cagcacgaag
     3061 tctggagacc tctggcggca gcctaccaag aacaactgga ccgaccggtg gtacctcacc
     3121 cttaccgagt cggcgacaca gtgtgggtcc gccgacacca gactaagaac ctagaacctc
     3181 gctggaaagg accttacaca gtcctgctga ccacccccac cgccctcaaa gtagacggca
     3241 tcgcagcttg gatacacgcc gcccacgtga aggctgccga ccccgggggt ggaccatcct
     3301 ctagactgcc gccaccatga gcgagctgat taaggagaac atgcacatga agctgtacat
     3361 ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta
     3421 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
     3481 cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg
     3541 catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac
     3601 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct
     3661 catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa
     3721 gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga
     3781 aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacatcaa
     3841 gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt
     3901 ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga
     3961 ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc tgaattccgg
     4021 actcagatct cgagctcaag ctttaatagt gtttgtcagg ttcaactcca gccatggttt
     4081 cccagtggag gtcgattctg acaccagcat cttccagctc aaggaggtgg ttgctaagcg
     4141 acagggggtt ccggctgacc agttgcgtgt gattttcgca gggaaggagc tgaggaatga
     4201 ctggactgtg cagaattgtg acctggatca gcagagcatt gttcacattg tgcagagacc
     4261 gtggagaaaa ggtcaagaaa tgaatgcaac tggaggcgac gaccccagaa acgcggcggg
     4321 aggctgtgag cgggagcccc agagcttgac tcgggtggac ctcagcagct cagtcctccc
     4381 aggagactct gtggggctgg ctgtcattct gcacactgac agcaggaagg actcaccacc
     4441 agctggaagt ccagcaggta gatcaatcta caacagcttt tatgtgtatt gcaaaggccc
     4501 ctgtcaaaga gtgcagccgg gaaaactcag ggtacagtgc agcacctgca ggcaggcaac
     4561 gctcaccttg acccagggtc catcttgctg ggatgatgtt ttaattccaa accggatgag
     4621 tggtgaatgc caatccccac actgccctgg gactagtgca gaatttttct ttaaatgtgg
     4681 agcacacccc acctctgaca aggaaacatc agtagctttg cacctgatcg caacaaatag
     4741 tcggaacatc acttgcatta cgtgcacaga cgtcaggagc cccgtcctgg ttttccagtg
     4801 caactcccgc cacgtgattt gcttagactg tttccactta tactgtgtga caagactcaa
     4861 tgatcggcag tttgtccacg accctcagct tggctactcc ctgccttgtg tggctggctg
     4921 tcccaactcc ttgattaaag agctccatca cttcaggatt ctgggagaag agcagtacaa
     4981 ccggtaccag cagtatggtg cagaggagtg tgtcctgcag atggggggcg tgttatgccc
     5041 ccgccctggc tgtggagcgg ggctgctgcc ggagcctgac cagaggaaag tcacctgcga
     5101 agggggcaat ggcctgggct gtgggtttgc cttctgccgg gaatgtaaag aagcgtacca
     5161 tgaaggggag tgcagtgccg tatttgaagc ctcaggaaca actactcagg cctacagagt
     5221 cgatgaaaga gccgccgagc aggctcgttg ggaagcagcc tccaaagaaa ccatcaagaa
     5281 aaccaccaag ccctgtcccc gctgccatgt accagtggaa aaaaatggag gctgcatgca
     5341 catgaagtgt ccgcagcccc agtgcaggct cgagtggtgc tggaactgtg gctgcgagtg
     5401 gaaccgcgtc tgcatggggg accactggtt cgacgtgtag ggatccaccg gatctagata
     5461 actgatcacg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     5521 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa
     5581 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata
     5641 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     5701 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     5761 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca
     5821 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     5881 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     5941 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
     6001 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     6061 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcacatgcag catgtatcaa
     6121 aattaatttg gttttttttc ttaagtattt acattaaatg gccatagttg cattaatgaa
     6181 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6241 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6301 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6361 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6421 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6481 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6541 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6601 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6661 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6721 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6781 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6841 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6901 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6961 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7021 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7081 ggatcttcac ctagatcctt ttgcggccgg ccgcaaatca atctaaagta tatatgagta
     7141 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7201 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7261 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7321 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7381 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7441 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7501 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7561 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7621 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7681 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7741 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7801 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7861 accgctgttg agatccagtt cga
//
