LOCUS       sequence-36790-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Fan W, Nassiri A, Zhong Q
  TITLE     Autophagosome targeting and membrane curvature sensing by 
            Barkor/Atg14(L).
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7769-74. doi: 
            10.1073/pnas.1016472108. Epub 2011 Apr 25.
  PUBMED    21518905
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1016472108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7769-74"
COMMENT     Sequence Label: Barkor Delta-10
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     287..306
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        287..305
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(360..425)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 agcgcataac agttacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
       61 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
      121 tgaacagact gtgaggactg aggggcctga aatgagcctt gggactgtga atctaaaata
      181 cacaaacaat tagaatcagt agtttaacac attatacact taaaaattgg atctccattc
      241 gccattcagg ctgcgcaact gttgggaagg gcgatcgagt gaattgtaat acgactcact
      301 atagggcgaa ttgggtacac ttacctggta ccctattatc tagatccggt ggatccctac
      361 ttgtcatcgt catccttgta gtcgatgtca tgatctttat aatcaccgtc atggtctttg
      421 tagtcgtcga ctgcagaatt cgaggtcacc gaggctgctg cagaggagat catcccacct
      481 gcactgctgc tcgcgatggg tggggacgcc tgggtgctct gactctggga gacttccaca
      541 gactgggaag ggatatcaca aaaccgggga ctaggcaagt tctcccagtc tgtgcccagg
      601 tcggtttctt catcgctgac gcgctcatct ccgctctcat ctgattctcc agcaactccg
      661 ggatccacaa attccatgga ctcctcaagg tctgctcgta cttcaaaggg ccctgacctg
      721 cctaggtgtt cagagcttgg actgaccagg tacattagat tcctgagggt atgcagtggt
      781 tgtaattgat ctaaatttac atgctgagaa aaacaaaggt aaagaatatt tgcattcagt
      841 ttcttcactg ctcgagtaaa tttctgcttg cttagatttt cgccacaaaa ttcactgttg
      901 cagagc
//
