LOCUS       sequence-368062-        9552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NAD+ biosensor comprised of a circularly permuted Venus fluorescent 
            protein (cpVenus), a bipartite NAD+-binding domain modeled from 
            bacterial DNA ligase, and a localization tag to target the nucleus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Cambronne XA, Stewart ML, Kim D, Jones-Brunette AM, Morgan RK, 
            Farrens DL, Cohen MS, Goodman RH
  TITLE     Biosensor reveals multiple sources for mitochondrial NAD(+).
  JOURNAL   Science. 2016 Jun 17;352(6292):1474-7. doi: 10.1126/science.aad5168.
  PUBMED    27313049
REFERENCE   2  (bases 1 to 9552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-17- 17"; volume: "352"; 
            issue: "6292"; pages: "1474-7"
COMMENT     Sequence Label: nuclear NAD+ biosensor
FEATURES             Location/Qualifiers
     source          1..9552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2850..2870
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2871..2894
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2907..2933
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2949..2973
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3741..3992
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     3929..3950
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4053..4074)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4314..4333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(4708..4732)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4745..5312
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4762..5314
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4906..4923)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5133..5152
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5326..5925
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5326..5345)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5822..5842
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5942..6530
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5995..6015)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6413..6424)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6605..6838
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6957..6975)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7043..7147
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7148..7216,7217..8008)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7366..7385)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8179..8767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8668..8687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8921..8938
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9178..9199
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9214..9219,9220..9237,9238..9244)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9252..9268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9257..9279
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9276..9292
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9276..9292
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9342..9361
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcagatct
     2821 ctcgaggttg atctgccacc atgaccggtc caaagaagaa gcgtaaggta gactacaagg
     2881 atgacgatga caagctcgat ggaggatacc catacgatgt tccagattac gctgctcgag
     2941 gttatcaaac aagtttgtac aaaaaagcag gctccaccat ggggcatatg gaaaaagctc
     3001 cgcatgacat tccaatgtat agtttaaatg atgggtttag caaggaagac atttttgctt
     3061 ttgatgaacg tgttcgtaag gcgattggta aacccgttgc ttactgttgt gagttactaa
     3121 ttgatgggtt agcaatttca ttacgttatg aaaacggggt gtttgtacgc ggagcaacac
     3181 gaggcgatgg aacggttggt gaaaacatta cagagaactt acgaacggtt cgctctgtac
     3241 cgatgcgttt aacggagccc atttcagtgg aagttcgtgg cgagtgctac atgcctaaac
     3301 aatcctttgt cgcattgaat gaagaacgtg aagaaaatgg ccaagatatt tttgccaatc
     3361 ctcggaatgc agcggcggga agcttgcgcc aactagatac aaaaattgtg gctaaacgga
     3421 acttaaatac gtttttatac acagttgctg actttggacc aatgaaagcg aagacgcagt
     3481 ttgaggcatt agaagagctt tctgcaattg gttttagaac caatcccgaa cggcaattat
     3541 gtcagtcaat tgatgaagtt tgggcctaca ttgaagaata ccatgaaaag cggagcactt
     3601 tgccatacga aatcaatgga attgtgatta aagtgaacga atttgcgttg caagatgaat
     3661 taggtttcac tgtcaaagca cctcgctggg cgattgccta caaatttcca tacaacagcg
     3721 acaacgtcta catcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     3781 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
     3841 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcttccag tccaagctga
     3901 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     3961 ggatcactct cggcatggac gagctgtaca agggcggttc cggaggcatg gtgagcaagg
     4021 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     4081 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     4141 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4201 tgggctacgg tctccaatgc ttcgcccgct accccgacca catgaagcag cacgacttct
     4261 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4321 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4381 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag cttgagtaca
     4441 acggcaccat agttctcgag ggtaccagat ctgaacaaca accattaaca ttaacggcgg
     4501 cgacaactcg tgcgcaagaa ttaagaaaac aactcaatca atattcccat gaatattatg
     4561 tcaaagatca accatccgta gaagattatg tttacgatcg tttgtataaa gagttagtcg
     4621 atattgaaac ggagtttcct gatttaatca cgccagattc gccgacacaa cgtgtaggtt
     4681 aaatcgatga gctctcgaga tatctagacc cagctttctt gtacaaagtg gttcgataac
     4741 gaattccgcc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     4801 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     4861 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     4921 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     4981 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     5041 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     5101 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     5161 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     5221 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     5281 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatgac cgagtacaag
     5341 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5401 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5461 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5521 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5581 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5641 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5701 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5761 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     5821 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     5881 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgatgcat cgatggatcc
     5941 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6001 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6061 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6121 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6181 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6241 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6301 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     6361 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6421 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6481 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct
     6541 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6601 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     6661 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6721 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6781 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6841 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6901 agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct
     6961 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     7021 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7081 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7141 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7201 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7261 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7321 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7381 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7441 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7501 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7561 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7621 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7681 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7741 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7801 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7861 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7921 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7981 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8041 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8101 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8161 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8221 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8281 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8341 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8401 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8461 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8521 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8581 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8641 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8701 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8761 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8821 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8881 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8941 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9001 cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9061 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9121 ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9181 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9241 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac
     9301 gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9361 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     9421 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
     9481 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
     9541 aaacttagta gt
//
