LOCUS       sequence-368080-        9686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Belanger KD, Walter D, Henderson TA, Yelton AL, O'Brien TG, Belanger
            KG, Geier SJ, Fahrenkrog B
  TITLE     Nuclear localisation is crucial for the proapoptotic activity of the
            HtrA-like serine protease Nma111p.
  JOURNAL   J Cell Sci. 2009 Nov 1;122(Pt 21):3931-41. doi: 10.1242/jcs.056887. 
            Epub 2009 Oct 6.
  PUBMED    19808885
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2009-11-1"; volume: "122(Pt 21):3931-41. doi"; pages: 
            " 10.1242/jcs.056887. Epub 2009 Oct 6"
COMMENT     Sequence Label: KBB462
FEATURES             Location/Qualifiers
     source          1..9686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        473..577
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(578..646,647..1438)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(796..815)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1609..2197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2098..2117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2351..2368
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2485..2506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2521..2526,2527..2544,2545..2551)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2559..2575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2564..2586
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2583..2599
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2583..2599
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2618..2638
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2620..2638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3047..3070
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             6082..6792
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(6109..6137)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6732..6752
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(6843..6861)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6857..6904
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(7282..7298)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7283..7299)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      7301..7548
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7317..7335)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(7566..7585)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7567..7585)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7595..7612)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7595..7611)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7604..7626)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7752..8207
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7839..7858)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8049..8070
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8338..9141)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9142..9357)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9381..9400)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9500..9522
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9560..9578)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(9619..9686,1..436)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 gtgtttattt atttttatgt tttgtatttg gattttagaa agtaaataaa gaaggtagaa
       61 gagttacgga atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt
      121 actttacata tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa
      181 cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac agacaagatg
      241 aaacaattcg gcattaatac ctgagagcag gaagagcaag ataaaaggta gtatttgttg
      301 gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt ctttaatttc
      361 tttttttact ttctattttt aatttatata tttatattaa aaaatttaaa ttataattat
      421 ttttatagca cgtgatgaaa aggacccagg tggcactttt cggggaaatg tgcgcggaac
      481 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      541 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      601 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      661 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      721 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      781 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      841 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      901 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      961 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1021 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1081 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1141 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1201 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1261 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1321 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1381 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1441 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1501 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1561 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1621 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1681 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1741 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1801 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1861 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1921 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1981 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2041 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2101 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2161 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2221 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2281 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     2341 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     2401 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     2461 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     2521 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     2581 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     2641 acaaaagctg gagctcgtga cagccctccg aaggaagact ctcctccgtg cgtcctcgtc
     2701 ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc cgcactgctc cgaacaataa
     2761 agattctaca atactagctt ttatggttat gaagaggaaa aattggcagt aacctggccc
     2821 cacaaacctt caaatgaacg aatcaaatta acaaccatag gatgataatg cgattagttt
     2881 tttagcctta tttctggggt aattaatcag cgaagcgatg atttttgatc tattaacgga
     2941 tatataaatg caaaaactgc ataaccactt taactaatac tttcaacatt ttcggtttgt
     3001 attacttctt attcaaatgt aataaaagta tcaacaaaaa attgttaata tacctctata
     3061 ctttaacgtc aaggagaaaa aactataatg accatatcgt tgagcaatat agcggcagca
     3121 gaccattcta aaatttccga tggaacttct ggtgaatcat cgcttgtagc agcggcacag
     3181 ttggaaagcg ccaccggaga tcaagaggaa gaatatacag accatgaaat cattatagag
     3241 ccgttgcact ttgctaataa taataacact gtactcacag attcagaaaa ttatctaaga
     3301 tggcaaaata ccatctccaa cgttgttaaa tcagtggttt ccattcattt ttcacaagtt
     3361 gcgccattcg attgcgattc tgctttggta tctgaagcta ccggatttgt cgtggatgct
     3421 aaattaggta taattctgac caataggcac gtcgttggtc ctggtccgtt cgtaggctac
     3481 gtagttttcg ataatcatga agaatgcgat gttataccaa tttacagaga tcctgttcat
     3541 gattttgggt tcctcaaatt cgatccgaaa aacataaaat attctaagat caaagcgtta
     3601 acgctaaaac caagtttggc taaggtcggc tcagaaatta gagttgtcgg taatgatgcc
     3661 ggtgagaaat taagtatcct agcaggtttt attagtagaa ttgacaggaa cgctccagaa
     3721 tatggtgagc tgacatacaa tgacttcaat acagaatata ttcaagctgc tgcatctgct
     3781 tctggcggtt caagtggttc tccagtcgta aatatagatg ggtatgctgt ggctttacag
     3841 gcaggtggct ctacagaggc atctactgat ttttttttac cgttagatag aattcttaga
     3901 gcgttaattt gcatccaaac caacaaacct atcacacgtg gtacgattca agttcaatgg
     3961 ctgctgaagc cttatgacga gtgtagaaga ttaggtttaa cttcagagag agaaagtgag
     4021 gctcgtgcta aatttcccga aaatatcgga ctgttggtag cagaaaccgt tttaagagaa
     4081 ggcccaggct atgataaaat taaagaaggt gatactctaa tttcaataaa cggtgaaacc
     4141 atatcttctt tcatgcaagt ggataaaatt caagatgaaa atgttgggaa ggaaatccaa
     4201 ttggttattc aaaggggcgg tgtcgaatgt actgtcacct gcactgttgg tgatttacat
     4261 gctatcaccc ctcatcgtta tgtagaggtt tgtggtgcga cttttcatga attatcatat
     4321 caaatggcaa ggttttatgc gctacctgtc aggggtgtat ttttaagtag tgcatcaggg
     4381 tctttcaact ttgattcaaa agaaagagtg ggttggattg tggattctat agacaataag
     4441 gaaacccccg acttagatac atttattgaa attatgaaaa ctattccaga tcgtaagcgc
     4501 gttactgtac gataccacca tctaacagat cagcattctc cacttgttac ttctatttac
     4561 attgaccgtc attggtgcaa tgaattcaga gtttacacaa ggaacgatac aactggtatt
     4621 tgggattaca aaaatgttgc tgatccactt ccagccgatg cgttgaaacc tcgttcggcc
     4681 aaaattattc ctattcctgt taataatgaa aaagttgcaa aattgtcatc atcgttatgt
     4741 acagtggcta caatggcagc agttccattg gattcacttt ctgctgatat tttaaaaaca
     4801 tcaggcttaa tcattgatgc tgaaaaaggt tatgtcctag tgtccagaag ggtagttcca
     4861 catgattgtc ttgatacttt tgtcaccatt gcagattccc ttgtagttcc agccacagta
     4921 gaatttctcc atccaactca caacttcgct attgttaaat atgatccaga gttagtaaag
     4981 gctccactaa tcactcccaa gctatctacc accagaatga aacgtggtga taagcttcag
     5041 tttataggtt ttacccagaa tgatagaatt gttacttcag agaccactgt caccgatatt
     5101 tcctctgtaa gtattccaag taatttaatt ccgaggtacc gtgccactaa tttagaagct
     5161 atatctatcg actgcaacgt tagcaccaga tgtaattccg gtattttgac agataatgat
     5221 ggtacagtaa gaggtttatg gcttccattt ttaggcgaaa gattagaaaa taaagaaaag
     5281 gtttatttga tgggattaga tataatggac tgtagggaag ttatagatat cttaaaaaat
     5341 ggcggtaaac caagagtaag tatagttgat gctgggttcg gttcaatttc cgttctgcaa
     5401 gccagaatta gaggtgtgcc cgaagaatgg atcatgcgta tggaacatga atcaaataac
     5461 aggctacagt ttatcactgt ttctagagtt tcttatacag aagacaagat tcatttagag
     5521 accggtgacg tcattttatc tgtgaacgga aaattagtca ctgaaatgaa tgatttgaat
     5581 ggagttgtaa gctcagctga tggtattttg ccatcagcca tgttagattt taaagttgtt
     5641 cgtgatggta atattgttga tttgaaaatt aaaaccgtgg aagttcagga aaccgaccgt
     5701 tttgtaatat ttgcaggtag tatcttacaa aagccgcatc atgctgtttt gcaagctatg
     5761 gttgatgttc caaagggtgt ctactgtaca ttcaggggag aatcgtcacc ggcgctccag
     5821 tacggtatat ctgccacaaa cttcataact catgtcaatg aaattgaaac acctgatcta
     5881 gatacatttt tgaaagttgt caaaacaatc cctgataata gttattgtaa aatgaggtta
     5941 atgactttcg acaatgtgcc cttcgctatt tcactgaaaa cgaattatca ttacttccct
     6001 actgcagaat tgaagaggga caacatcacg cacaaatgga ttgaaaagga atttaccggc
     6061 aacagccaaa gtgaaaaagc tagcaaagga gaagaactct tcactggagt tgtcccaatt
     6121 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
     6181 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
     6241 gttccatggc caacacttgt cactactttc acttatggtg ttcagtgctt ttcaagatac
     6301 ccggatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     6361 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
     6421 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
     6481 aacattcttg ggcacaaatt ggaatacaac tataactcac acaatgtata catcatggca
     6541 gacaaacaaa agaatggaat caaagctaac ttcaaaatta gacacaacat tgaagatgga
     6601 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     6661 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     6721 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     6781 gaactataca aaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     6841 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     6901 tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg
     6961 cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt
     7021 cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca
     7081 gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca
     7141 gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga
     7201 tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata
     7261 aactaccgca ttaaagctta tcgataccgt cgacctcgag tcatgtaatt agttatgtca
     7321 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
     7381 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
     7441 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
     7501 aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg ccggtaccca
     7561 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     7621 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     7681 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     7741 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7801 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7861 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7921 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7981 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8041 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8101 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8161 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat
     8221 tttctcctta cgcatctgtg cggtatttca caccgcatag ggtaataact gatataatta
     8281 aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta
     8341 gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc
     8401 ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga
     8461 tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc
     8521 atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat
     8581 gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt
     8641 acccttagta tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa
     8701 aaggcctcta ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc
     8761 tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc
     8821 cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag
     8881 taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa
     8941 atcagtcaag atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac
     9001 taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt
     9061 ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc
     9121 cttatatgta gctttcgaca tgatttatct tcgtttcctg caggtttttg ttctgtgcag
     9181 ttgggttaag aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac
     9241 caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa
     9301 aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaaaag
     9361 gtggtatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     9421 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     9481 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     9541 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     9601 ataatggttt cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat
     9661 gatggaataa tttgggaatt tactct
//
