LOCUS       sequence-368134-       10093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NUP82 expression in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10093)
  AUTHORS   Belanger KD, Simmons LA, Roth JK, VanderPloeg KA, Lichten LB, 
            Fahrenkrog B
  TITLE     The karyopherin Msn5/Kap142 requires Nup82 for nuclear export and 
            performs a function distinct from translocation in RPA protein 
            import.
  JOURNAL   J Biol Chem. 2004 Oct 15;279(42):43530-9. doi: 
            10.1074/jbc.M407641200. Epub 2004 Aug 4.
  PUBMED    15294903
REFERENCE   2  (bases 1 to 10093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M407641200"; journalName: "J Biol Chem"; date: 
            "2004-10-15- 15"; volume: "279"; issue: "42"; pages: "43530-9"
COMMENT     Sequence Label: KBB329
FEATURES             Location/Qualifiers
     source          1..10093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..504
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        541..645
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(646..714,715..1506)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(864..883)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1677..2265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2166..2185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2419..2436
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2553..2574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2589..2594,2595..2612,2613..2619)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2627..2643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2632..2654
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2651..2667
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2651..2667
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2688..2706
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2734..2750
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2735..2751
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7996..8012)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7996..8012)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8044..8063)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8045..8063)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8070..8087)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8070..8086)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8079..8101)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8227..8682
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8314..8333)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8524..8545
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8813..9616)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9617..9832)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9856..9875)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9975..9997
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10035..10053)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatcgcttgc ctgtaactta cacgcgcctc gtatctttta atgatggaat aatttgggaa
       61 tttactctgt gtttatttat ttttatgttt tgtatttgga ttttagaaag taaataaaga
      121 aggtagaaga gttacggaat gaagaaaaaa aaataaacaa aggtttaaaa aatttcaaca
      181 aaaagcgtac tttacatata tatttattag acaagaaaag cagattaaat agatatacat
      241 tcgattaacg ataagtaaaa tgtaaaatca caggattttc gtgtgtggtc ttctacacag
      301 acaagatgaa acaattcggc attaatacct gagagcagga agagcaagat aaaaggtagt
      361 atttgttggc gatcccccta gagtctttta catcttcgga aaacaaaaac tattttttct
      421 ttaatttctt tttttacttt ctatttttaa tttatatatt tatattaaaa aatttaaatt
      481 ataattattt ttatagcacg tgatgaaaag gacccaggtg gcacttttcg gggaaatgtg
      541 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      601 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      661 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      721 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      781 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      841 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      901 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      961 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1021 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1081 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1141 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     1201 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1261 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     1321 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     1381 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1441 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1501 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1561 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1621 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1681 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1741 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1801 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1861 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1921 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1981 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2041 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2101 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2161 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2221 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2281 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2341 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2401 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     2461 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     2521 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2581 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2641 caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac
     2701 taaagggaac aaaagctggt accgggcccc ccctcgaggt cgacggtatc gataagcttc
     2761 ctgtagaggg gatggagatg caaaacaaat tcttgtatgt gccttagctg cataacttaa
     2821 caactctgcg ttcaaggata agacccaacc aattctccaa ccagtagcag caaacgattt
     2881 accggcagaa ccgaccgtta aggttagttg cccaatttct ggagagagtg tggcaattct
     2941 agtgaaagaa tcagtgaagt aaaggtgttc atagacttca tcagatataa tcacaacgtt
     3001 gtgcttgacg caaatgttac ctaaagtggt taattcctcg cgcgtgaaaa ctttaccaat
     3061 tgggttgtga ggggtattga taatgacagc ttttgtcttg gatgtaatcg ctttttcgaa
     3121 ctgctcaaag tcaatggtcc attcttcacc tctagtattc ctttgatcca attcctttgg
     3181 aggatttatg gggacgtaaa caactttacc accgcaaagt tcgatatttg gaatatattg
     3241 gtcaaagaaa ggttcaaaaa caataacctc gtcgccagcg ttcaaaagcc ccatcaagca
     3301 agaaagtata ccttcattgg cacctgttgt tacggtaaca ttttccgctt tcaattctgt
     3361 gttataaata ggagaataca acttaatcaa ggaattaatt aatgaaggtc gacctctagt
     3421 tggagaatat tgattgacca ttggaatgtc tagggctttc tgagcctcct taatggcgaa
     3481 ttgaggaggg gaatatgaaa aaaagccttg gcctaaatta ataagttcac ggccttggtt
     3541 tttggagtta ttggcagctt ttgcagcggc ttcattggtt agcgaccaaa catctttggc
     3601 agtgttagaa gtgaaatatt tgttggcaac aactttcggt ctcgaagtag acattaggtt
     3661 cgtaaattga cgaatgaatc gttgtttcat tgagagcaag taacaatgga ttctatacgc
     3721 aattatagta aattgaacac tttatatata tctctatcgt accttcccct tttaaacatg
     3781 actgaatttt ggtatcttga ttgtcataat gacaacaaaa aagccacagt tctacgtcac
     3841 gtgctagaca cgacatgcta ctgaagaaat tgttatcaaa tgaaaagata catttatgta
     3901 ataaccacta ccaggcactt gctaaaaaga atctacgtaa gaactacata aaggttagct
     3961 tgtgcgccag aaagtgatat caaagtttta tgaacgctga acaattttga gccagtaatt
     4021 atcttggcat aggagacgat aaatgccatc gttcttttaa agagcttatt aagttattaa
     4081 ctctcttcga ttagacattc tcttatcaaa cgagttacgt tacatttagg aataatgcga
     4141 caaagatgga ggtcttgatt cctaaatcct actcaaaaca atctgctcta ctgtcaaaat
     4201 ccttgctccg taaattgatt tcgttgtcag acgctcaccg tacaagaagt ttgtgggtat
     4261 ttacatcgta agaaaagtgc aaccagattc aatattgaat tacctgagcg gtgtcttcct
     4321 atattaaaat gacgattttg tttttctttg tattgtgagg gtagcagatt cgtcttctgc
     4381 acttttcatc tactattgca aagcctaata caaatacagt tgttgaatat aaaaattcga
     4441 aatcagcctt ccattctcga aagtgaacaa ggaaactcac tggataaaaa ttcacacgaa
     4501 aagaaaaaac cgaaaaaaag taactgtgtt gcttgcagag ggtttgatta ctcataaccg
     4561 agagacacga tctgtagcgg tgatatgaac gtattcctca ccttcttcta accctttgga
     4621 ataatagttt ccatcactct ttacaaaagt agcattactc accaaataag gtcataaaaa
     4681 agacttttat tttgtataca tgtaaatttt ggcagcggtt agtgaggtaa aagagatacg
     4741 aatgaatata cgatacaatg atacagcata ctgaaaaatc atcctaacga tttggtgcac
     4801 agaaggcttt atatattgac caattcatat ataatgtcct cttgctacat gatattatac
     4861 cattgcccag ttgtttctta gcgtacatat atgatagcag actatgcaag tcgcttactc
     4921 attgagtttt agtagtaaat tcctgagaaa cttgtaacaa ttcttcattg cattctttaa
     4981 ttatctttga atctatttca agcattttgc gtagctcatc ccattcgttt tggctctttt
     5041 tatcgacttt gatagtttct gcctcgatac gagttagttc actcttcaag tacgataaat
     5101 cttgttgaag ggatttttga gagtgaacaa agctgttgaa ttgcaatatt tggtttcgta
     5161 tctctttgaa ccatttcatc tcaccatggc ttattttctt ttctttaaat ttattggatt
     5221 cggcgatttg agatagcttc ttgctcaatt tactaaatct ctccattaat tctgactgtc
     5281 tgcttagttg tgcatcccat ttcttatttt gtgcttcgaa ttttctcctt aaatcatcat
     5341 cctttgaaat tattttgcat gtcgattgta gttggcgcgt caattcgaac tgttgctcat
     5401 gaattctatt gtggatagaa acacccaaag tttgagcttt aacaattctt tgcaaaaatt
     5461 ctttggaaat atccgtgaaa atttccaact gattttcact agcttcgttt tttaaaggta
     5521 tctggcggtc tgctgatggg ataattcgtt cacatggact aatacaggct ttttgaaaat
     5581 tatcgttcaa aataagaatc tcatttatgg gctgtgtgaa actcatttta aaaccatctc
     5641 ctaccgtgtc acttttttct gtactgatac ttgtttcagc tgcggtgctt tgcggtttca
     5701 tatcgcttga aatattttga aaagtcaatg ttttattcga catcaaggct aaattagatt
     5761 ggtcattcca gttgatatac gctaggtttg gaattctttc tatggtggca atatcctcaa
     5821 gtttactttc aaatttcaat cctgcaagtg gattcaaatc cgattcgttg atacatttac
     5881 tcaatgtaga agcccagctc ataaaattta cttgttgtat aatattatcg gaaatgacat
     5941 aaagttttcc caactgttca ggtaacgtta ttagagactt aatttctctt tgtagtttga
     6001 ctcgttcaat tagaaccaaa gaattattat acacgtaatt gtcaacatcc cacgacattg
     6061 acatttccaa atctttgaag agtaagatta aagacccatc atcaaaactg acacaaacaa
     6121 tttcattttg cccattatca attagtatgg ttgcaatatt tgtagcagta tagtcataca
     6181 gctcgccggg aaaggggttt atcgtgaatg gtccttgaac cttggctaaa cgatattcct
     6241 tttgtatgtc gactttgcca aatcttgaat tccaattttc atgcagtttt gacacaaact
     6301 gtaactgctt aataacattc cttttaacta taacatctgt tgtagaatcc agggactcgt
     6361 acattactaa agatttgttt aggatcaagt tgagatcttt ctcatttaac agtaatactg
     6421 acggtaaaaa tggataaaat gcaaagatat caccgccttc tgtggtgttt agacaatata
     6481 acgtcaatcc gtccttactg aattctaaat ctgtgatgtc gttaacacgt gcatctaaac
     6541 caaaggagtt gttcggtttg ttcaaaacga taggcttttc ttgcgaatta aggatgtcaa
     6601 acatcgtgat agtgtcatcc tctttcaaaa ccactataca agaatcacga taactctttg
     6661 gatgaaacaa aactttctta atggacgatt tcggaccaac ttcttcttca tcaattgaat
     6721 agtggaaaat ttggaatgca tcttgaatgg aaacgtcttc aacgttggaa tagccccaag
     6781 gaacttccat tacaaaaatc tcgttgtcat tgaaaagaca gagtaggtca ccagacgtac
     6841 tagatattac atggaaggta tcatccaaga cgagatgacg actaaagttc aaactgtggt
     6901 aaagtgaatc agtgagtaca ttgtaccacc ttattatgtt atcttgaatg aaaacaatcc
     6961 ttgtgccatt ctgagaacta aaaatgtaac gtggagattg gcttgccgaa agcgatgctt
     7021 ggaaaatagg aagtgcactt aacctactag attgggacat actttggagt atagaattaa
     7081 gccaccactc aattatcgct gtccttttgt tgcttaaata ttaaatgcaa ttagaatatt
     7141 tgaaagtatt caactttttt attgaagtta atttttcatt tttgctgccg cgaaaagaga
     7201 gaaggtcgat agtagatttt ttttttttca cttgtctcag tatgaatcta taaactatca
     7261 tatatgtata catatttaga ccacgtgaac aattctatgt taatcaagag cgcaaaggag
     7321 ggccaattca ggtaaccagc cattcagcaa tcccatcaca aaattccacc caagaaaata
     7381 acataatttt ggactgaaaa cggaccaaat aaagagatga aatctcaaaa cgacgcatgc
     7441 cgtcatcaaa caagttccaa atatacaatg ataggtgaag gcaggtaact tacttctgat
     7501 gaacgtttgc aacttaactt gtgctggaat agaggcgtag tttattgacc acggtagcat
     7561 ggaccagtat attgccggcg cgaaattggc aacggacatt aatatcccca ctacgtagat
     7621 gttgtagtct gaggaaacac catggtatgc gtttccaagg tctatagtag aaatggaatt
     7681 tgtgccaccg aattggaaaa atgtaaaatt ttgtaaaatt aaggacacca aattcgaaaa
     7741 ataaatctta cttgttgatg ttaatgaggc ggttggaata ttttggaaaa agtaaaaaat
     7801 ttgggactct aatatttgga aaacgagaaa ttgacctatg ttctgagaag aagtttggaa
     7861 catcaaaaga attgttatat aaactttcat atctttaatg aggtagttct tgttaagtac
     7921 tttcaatttc gtcaggacca accttgaaat gacaacaata gcccaagctt gatatcgaat
     7981 tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg gagctccaat
     8041 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     8101 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8161 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8221 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8281 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8341 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8401 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8461 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8521 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8581 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8641 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     8701 ccttacgcat ctgtgcggta tttcacaccg catagggtaa taactgatat aattaaattg
     8761 aagctctaat ttgtgagttt agtatacatg catttactta taatacagtt ttttagtttt
     8821 gctggccgca tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt tcaccctcta
     8881 ccttagcatc ccttcccttt gcaaatagtc ctcttccaac aataataatg tcagatcctg
     8941 tagagaccac atcatccacg gttctatact gttgacccaa tgcgtctccc ttgtcatcta
     9001 aacccacacc gggtgtcata atcaaccaat cgtaaccttc atctcttcca cccatgtctc
     9061 tttgagcaat aaagccgata acaaaatctt tgtcgctctt cgcaatgtca acagtaccct
     9121 tagtatattc tccagtagat agggagccct tgcatgacaa ttctgctaac atcaaaaggc
     9181 ctctaggttc ctttgttact tcttctgccg cctgcttcaa accgctaaca atacctgggc
     9241 ccaccacacc gtgtgcattc gtaatgtctg cccattctgc tattctgtat acacccgcag
     9301 agtactgcaa tttgactgta ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa
     9361 aattgtactt ggcggataat gcctttagcg gcttaactgt gccctccatg gaaaaatcag
     9421 tcaagatatc cacatgtgtt tttagtaaac aaattttggg acctaatgct tcaactaact
     9481 ccagtaattc cttggtggta cgaacatcca atgaagcaca caagtttgtt tgcttttcgt
     9541 gcatgatatt aaatagcttg gcagcaacag gactaggatg agtagcagca cgttccttat
     9601 atgtagcttt cgacatgatt tatcttcgtt tcctgcaggt ttttgttctg tgcagttggg
     9661 ttaagaatac tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc
     9721 taagtctgtg ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat
     9781 ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aaaaggtggt
     9841 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     9901 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     9961 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    10021 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
    10081 ggtttcttag acg
//
