LOCUS       sequence-368341-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Booster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for 
            monitoring PKA activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Watabe T, Terai K, Sumiyama K, Matsuda M
  TITLE     Booster, a Red-Shifted Genetically Encoded Forster Resonance Energy 
            Transfer (FRET) Biosensor Compatible with Cyan Fluorescent 
            Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue 
            Light-Responsive Optogenetic Tools.
  JOURNAL   ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941.
  PUBMED    32101394
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Sens. 
            2020 Feb 26. doi: 10.1021/acssensors.9b01941."
COMMENT     Sequence Label: pCAGGS-4493NES
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(391..738)
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             complement(487..834)
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
ORIGIN
        1 ggnnccgtcc ntgtagtacc tcatcttcat ctctggttta atcacactca ccattccgga
       61 accaccagca ctaccaccag cactaccacc agcactacca ccagcactac caccagcact
      121 ggtactacca ccagcactac caccagcact gccggaacca ccagcactac caccagcact
      181 accaccagca ctaccaccag cactggtact accaccagca ctaccaccag cactgccgga
      241 accaccagca ctaccaccag cactaccacc agcactacca ccagcactac caccagcact
      301 ggtactacca ccagcactac caccagcact accaccagca ctaccaccag cactggtact
      361 accaccagca ctaccaccag cactgccgga accaccagca ctaccaccag cactaccacc
      421 agcactacca ccagcactac caccagcact ggtactacca ccagcactac caccagcact
      481 gccggaacca ccagcactac caccagcact accaccagca ctaccaccag cactaccacc
      541 agcactggta ctaccaccag cactaccacc agcactgccg gaaccaccag cactaccacc
      601 agcactacca ccagcactac caccagcact accaccagca ctggtactac caccagcact
      661 accaccagca ctgccggaac caccagcact accaccagca ctaccaccag cactaccacc
      721 agcactacca ccagcactgg tactaccacc agcactacca ccagcactgc cggaaccacc
      781 agcactacca ccagcactac caccagcact accaccagca ctaccaccag cactggtacc
      841 gcgatcaact ttgttctgct cnaggcactg cttaaatttg tcgtttatga aaatgaccng
      901 agataaaata tctgattcca cgcctacacc aacggnnatt tcatcacntt gggacagtaa
      961 ctgantgctg ntcttctcga ctttttgccc anttaaccag nnncna
//
