LOCUS       sequence-368402-        2568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 donor AF - CEN ori from yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: AF_CEN
FEATURES             Location/Qualifiers
     source          1..2568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    231..734
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     rep_origin      complement(881..1469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(961..980)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1560..2351)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
       61 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
      121 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctca
      181 ttactcgcat ccattctcag gctgtctcgt ctcgtctcag gtctcaggag atcacgtgct
      241 ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa atagaaagta
      301 aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag actctagggg
      361 gatcgccaac aaatactacc ttttatcttg ctcgtcctgc tctcaggtat taatgccgaa
      421 ttgtttcatc ttgtctgtgt agaaaaccac acacgaaaat cctgtgattt tacattttac
      481 ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata aatatatatg
      541 taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt cttcattccg
      601 taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat aaaaataaat
      661 aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg cgtgtaagtt
      721 acaggcaagc gatccgtccg cttgagacca ccaggataca tagattacca caactccgag
      781 cccttccacc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      841 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      901 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      961 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1021 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1081 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1141 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1201 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1261 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1321 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1381 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1441 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1501 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtgattat
     1561 tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga
     1621 tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt
     1681 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc
     1741 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt
     1801 cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc
     1861 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag
     1921 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct
     1981 ggctcgaaga taccagcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc
     2041 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg
     2101 cggagaatct cactctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct
     2161 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt
     2221 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg
     2281 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc
     2341 gcttccctca tgcgaaacga tcctcatcct gtctcttgat cagatattga tcccctgcgc
     2401 catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta
     2461 ccagagggcg ccccagctgg caattccggt tcgcttgcta agcttgcatg cctgcaagtc
     2521 gactctagag gagcggtgat cacaggcagc aacgctctgt catcgtta
//
