LOCUS       sequence-368410-        2262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 donor AF - spacer DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: AF_Spacer2
FEATURES             Location/Qualifiers
     source          1..2262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             771..1562
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1653..2241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2142..2161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
       61 gattctgtgg gtggaagggc tcggagttgt ggtaatctat gtatcctggg gtggtctcga
      121 gcgtggcgat cgggcttcag ttcaggtcca gtttcgtttg tcgtgagttt ctctaagctt
      181 ttaccgcgag tccaagccct ccataaaggg ttctggcagt gttctattaa aatagggtgt
      241 aggtctattg gtattacagt ttcattttct tgcgggtaag gaagggttcc cctaatcact
      301 aacgcacgat gtttccctcc agcctccgga gacctgagac gagacgagac agcctgagaa
      361 tggatgcgag taatgagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
      421 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
      481 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
      541 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaccgct cctctagagt
      601 cgacttgcag gcatgcaagc ttagcaagcg aaccggaatt gccagctggg gcgccctctg
      661 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
      721 ggcgcagggg atcaatatct gatcaagaga caggatgagg atcgtttcgc atgagggaag
      781 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      841 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      901 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      961 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagt gagattctcc
     1021 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     1081 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgctggt atcttcgagc
     1141 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     1201 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     1261 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     1321 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1381 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1441 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1501 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     1561 aataatcacc tagaatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1621 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1681 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1741 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1801 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1861 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1921 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1981 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2041 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2101 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2161 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2221 ggggggcgga gcctatggaa aaacgccagc aacgcggcct tt
//
