LOCUS       sequence-368414-        3119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 donor AF - URA3 selectable marker from yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: AF_URA3
FEATURES             Location/Qualifiers
     source          1..3119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             796..1587
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1678..2266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2167..2186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2487..3119,1..171))
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac
       61 atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc
      121 aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct
      181 tcgtttcctg cacgtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt
      241 ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc
      301 ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgct cctgagacct
      361 gagacgagac gagacagcct gagaatggat gcgagtaatg agctcggcac aaaatcacca
      421 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
      481 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
      541 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
      601 cctgtgatca ccgctcctct agagtcgact tgcaggcatg caagcttagc aagcgaaccg
      661 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
      721 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa tatctgatca agagacagga
      781 tgaggatcgt ttcgcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      841 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      901 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
      961 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     1021 tcccctggag agagtgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     1081 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     1141 gacattcttg ctggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     1201 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     1261 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     1321 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1381 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1441 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1501 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     1561 gagatcacca aggtagtcgg caaataataa tcacctagaa tgcatgacca aaatccctta
     1621 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1681 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1741 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1801 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1861 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1921 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1981 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2041 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2101 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2161 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2221 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2281 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2341 atcccctgat tctgtgggtg gaagggctcg gagttgtggt aatctatgta tcctggtggt
     2401 ctcaagcggg gtaataactg atataattaa attgaagctc taatttgtga gtttagtata
     2461 catgcattta cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct
     2521 tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat
     2581 agtcctcttc caacaataat aatgtcagat cctgtagaaa ccacatcatc cacggttcta
     2641 tactgttgac ccaatgcatc tcccttgtca tctaaaccca caccgggtgt cataatcaac
     2701 caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa
     2761 tctttgtcgc ttttcgcaat gtcaacagta cccttagtat attctccagt agatagggag
     2821 cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct
     2881 gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg
     2941 tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca
     3001 atgtcagcaa attttctgtc ctcgaagagt aaaaaattgt acttggcgga taatgccttt
     3061 agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttag
//
