LOCUS       sequence-368439-        9577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-On inducible expression of iPAK4 in a lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Lin D, Li X, Moult E, Park P, Tang B, Shen H, Grimm JB, Falco N, Jia
            BZ, Baker D, Lavis LD, Cohen AE
  TITLE     Time-tagged ticker tapes for intracellular recordings.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7.
  PUBMED    36593408
REFERENCE   2  (bases 1 to 9577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7."
COMMENT     Sequence Label: DL145_plenti_TRE_iPAK4
FEATURES             Location/Qualifiers
     source          1..9577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      179..314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     241..260
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        376..423
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             442..816
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    946..1067
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(983..1002)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1037..1056
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1116..1132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1116..1132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1129..1151)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1140..1156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1164..1170,1171..1188,1189..1194))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1209..1230
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1347..1364)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1518..2106)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1598..1617)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2277..3068,3069..3137))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2900..2919
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3138..3242)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3317..3336)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3508..3887
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3889..4087
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4105..4285
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4332..4457
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4950..5183
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5368..5412
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5710..5827
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     5997..6019
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6011..6028
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6012..6028
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6038..6057
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6038..6056
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        6081..6395
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    6089..6107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6125..6143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6160..6178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6196..6214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6232..6250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6267..6285
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6303..6321
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6368..6392
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6406..6415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6415..6438
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7539..8127
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7592..7612)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8010..8021)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8130..8146)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8131..8147)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8652..8832
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8858..8875)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8864..9088
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9134..9562
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9221..9240)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9431..9452
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(join(9571..9577,1..14))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(9576..9577,1..328)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
       61 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
      121 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
      181 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
      241 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
      301 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
      361 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
      421 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
      481 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
      541 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
      601 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
      661 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
      721 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
      781 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
      841 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
      901 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
      961 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1021 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     1081 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1141 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1201 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1261 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1321 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1381 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1441 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1501 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1561 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1621 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1681 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1741 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1801 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1861 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1921 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1981 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2041 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2101 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2161 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2221 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2281 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2341 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2401 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2461 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2521 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2581 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2641 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2701 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2761 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2821 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2881 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2941 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3001 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3061 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3121 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3181 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3241 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     3301 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     3361 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     3421 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     3481 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     3541 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3601 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3661 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3721 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3781 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3841 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3901 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3961 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     4021 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     4081 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     4141 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4201 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4261 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     4321 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     4381 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     4441 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     4501 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     4561 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     4621 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     4681 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     4741 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     4801 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     4861 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     4921 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     4981 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     5041 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     5101 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     5161 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     5221 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     5281 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     5341 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     5401 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     5461 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     5521 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     5581 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     5641 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
     5701 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     5761 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     5821 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta accattcgcc
     5881 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     5941 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     6001 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
     6061 attggctttc gtcttcactc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     6121 ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac
     6181 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
     6241 gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt
     6301 actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata
     6361 taagcagagc tcgtttagtg aaccgtcaga tcgcctggaa ccggtgccac catggactac
     6421 aaggacgacg atgataagtc cggatccgaa gcagaggact ggacagcagc cctactgaac
     6481 aggggtcgca gtcgccagcc cctggtacta ggggacaatt gctttgctga cttggtgcac
     6541 aactggatgg agctgcctga ggaattcccc gccgcccctg ctgttcctgg gccccctggc
     6601 ccccgctcac cacagcggga gccacagcga gtatcccatg agcagttccg ggctgccctg
     6661 cagctggtgg tggacccagg cgacccccgc tcctacctgg acaacttcat caagattggc
     6721 gagggctcca cgggcatcgt gtgcatcgcc accgtgcgca gctcgggcaa gctggtggcc
     6781 gtcaagaaga tggacctgcg caagcagcag aggcgcgagc tgctcttcaa cgaggtggta
     6841 atcatgaggg actaccagca cgagaatgtg gtggagatgt acaacagcta cctggtgggg
     6901 gacgagctct gggtggtcat ggagttcctg gaaggaggcg ccctcaccga catcgtcacc
     6961 cacaccagga tgaacgagga gcagatcgca gccgtgtgcc ttgcagtgct gcaggccctg
     7021 tcggtgctcc acgcccaggg cgtcatccac cgggacatca agagcgactc gatcctgctg
     7081 acccatgatg gcagggtgaa gctgtcagac tttgggttct gcgcccaggt gagcaaggaa
     7141 gtgccccgaa ggaagtcgct ggtcggcacg ccctactgga tggccccaga gctcatctcc
     7201 cgccttccct acgggccaga ggtagacatc tggtcgctgg ggataatggt gattgagatg
     7261 gtggacggag agccccccta cttcaacgag ccacccctca aagccatgaa gatgattcgg
     7321 gacaacctgc caccccgact gaagaacctg cacaaggtgt cgccatccct gaagggcttc
     7381 ctggaccgcc tgctggtgcg agaccctgcc cagcgggcca cggcagccga gctgctgaag
     7441 cacccattcc tggccaaggc agggccgcct gccagcatcg tgcccctcat gcgccagaac
     7501 cgcaccagat gagcggccgc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     7561 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     7621 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     7681 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7741 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7801 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7861 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7921 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     7981 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     8041 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     8101 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     8161 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     8221 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     8281 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8341 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     8401 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     8461 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     8521 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     8581 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     8641 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8701 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8761 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8821 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     8881 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     8941 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     9001 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     9061 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     9121 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9181 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9241 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9301 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9361 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9421 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     9481 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     9541 taacaaaaat ttaacgcgaa ttaattctgt ggaatgt
//
