LOCUS       sequence-368443-        4358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal mScarlet with flexible linker (GGGGS)x3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Nikonorova IA, Wang J, Cope AL, Tilton PE, Power KM, Walsh JD, 
            Akella JS, Krauchunas AR, Shah P, Barr MM
  TITLE     Isolation, profiling, and tracking of extracellular vesicle cargo in
            Caenorhabditis elegans.
  JOURNAL   Curr Biol. 2022 May 9;32(9):1924-1936.e6. doi: 
            10.1016/j.cub.2022.03.005. Epub 2022 Mar 24.
  PUBMED    35334227
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.03.005"; journalName: "Curr Biol"; date: 
            "2022-05-9- 9"; volume: "32"; issue: "9"; pages: "1924-1936.e6"
COMMENT     Sequence Label: pIN1_GGGSx3_mScarlet
FEATURES             Location/Qualifiers
     source          1..4358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2918..2940)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3040..3059
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3137..3155)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3223..3327
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3328..3396,3397..4188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3546..3565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgt aagtttaaac
     1021 atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc
     1081 actcacaacg atggatacgc taacaacttg gaaatgaaat aagcttgcat gcctgcaggt
     1141 cgactctaga ggatccccgg gattggccaa aggacccaaa ggtatgtttc gaatgatact
     1201 aacataacat agaacatttt caggaggacc cttggagggt accggtagaa aaaggaggtg
     1261 gcggatctgg aggtggaggc tctggaggag gtggatctgt cagcaaggga gaggcagtta
     1321 tcaaggagtt catgcgtttc aaggtccaca tggagggatc catgaacgga cacgagttcg
     1381 agatcgaggg agagggagag ggacgtccat acgagggaac ccaaaccgcc aagctcaagg
     1441 tcaccaaggg aggaccactc ccattctcct gggacatcct ctccccacaa ttcatgtacg
     1501 gatcccgtgc cttcatcaag cacccagccg acatcccaga ctactacaag caatccttcc
     1561 cagagggatt caagtgggag cgtgtcatga acttcgagga cggaggagcc gtcaccgtca
     1621 cccaagacac ctccctcgag gacggaaccc tcatctacaa ggtcaagctc cgtggaacca
     1681 acttcccacc agacggacca gtcatgcaaa agaagaccat gggatgggag gcctccaccg
     1741 agcgtctcta cccagaggac ggagtcctca agggagacat caagatggcc ctccgtctca
     1801 aggacggagg acgttacctc gccgacttca agaccaccta caaggccaag aagccagtcc
     1861 aaatgccagg agcctacaac gtcgaccgta agctcgacat cacctcccac aacgaggact
     1921 acaccgtcgt cgagcaatac gagcgttccg agggacgtca ctccaccgga ggaatggacg
     1981 agctctacaa gtagcattcg tagaattcca actgagcgcc ggtcgctacc attaccaact
     2041 tgtctggtgt caaaaataat aggggccgct gtcatcagag taagtttaaa ctgagttcta
     2101 ctaactaacg agtaatattt aaattttcag catctcgcgc ccgtgcctct gacttctaag
     2161 tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt
     2221 tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc
     2281 taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa
     2341 attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg
     2401 tacacttctt atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc
     2461 acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg
     2521 cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt
     2581 ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg
     2641 ggtttcccct attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag
     2701 tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa
     2761 ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat
     2821 gacagaatac attcccaata taccaaacat aactgtttcc tactagtcgg ccgtacgggc
     2881 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     2941 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     3001 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     3061 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     3121 tcaggcggcc ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3181 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3241 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3301 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3361 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3421 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3481 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3541 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3601 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3661 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3721 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3781 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3841 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3901 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3961 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4021 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4081 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4141 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4201 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4261 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4321 tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
