LOCUS       sequence-368446-        6776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter 
            and the BBa_B0032 RBS, for the expression in Synechocystis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Mascia F, Pereira SB, Pacheco CC, Oliveira P, Solarczek J, Schallmey
            A, Kourist R, Alphand V, Tamagnini P.
  TITLE     Light-driven hydroxylation of testosterone by Synechocystis sp. PCC 
            6803 expressing the heterologous CYP450 monooxygenase CYP110D1
  JOURNAL   Green Chemistry, 24 (2022):6156
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Green 
            Chemistry, 24 (2022):6156"
COMMENT     Sequence Label: pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1
FEATURES             Location/Qualifiers
     source          1..6776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..31
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     misc_feature    121..142
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        join(149..154,155..171,172..178)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     terminator      1659..1753
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1874..1893)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1885..2700
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2867..2975
                     /note="incP origin of transfer"
     CDS             complement(2986..3837)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(3824..4663)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(5174..6145)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      6252..6646
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(6684..6770)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccgggcta attagggggt gtcgcccttt acacgtactt agtcgctgaa
      121 gaattcgcgg ccgcttctag aggagctgtt gacaattaat catccggctc gtataatgtg
      181 tggatccccg tcagtgacgg agattcacac atactagtca cacaggaaag tactagatga
      241 cagtcactca aaacctaccc aacggcccca gaataccccg gttgttacgg ttgtttaagt
      301 ttattactca accaatacag tatgtggaag attttgccaa agtctacggt gacaacttca
      361 ctatctgggg gagtggagaa agttattttg tctactttag ccatccccaa gcattggaac
      421 agatttttac taatgttagt tgttttgaga gtagtggtgg aggtagtccg cttttagaac
      481 ttttattggg taagaattct ttaattttac tagaaggcga tcgccatcag cgccaacgtc
      541 aactattaac cccacctttc cacggtgaaa ggatgcgggc ttatggtcaa actattcgtg
      601 aaataactca acaagtcact caagcatggc aaatgggtaa gcccttcaat atccgggctt
      661 ccatgcagga aattaccatg cgtgtcattt tgcgggtagt gtttggtgtt gatgaggggg
      721 aattgtttca agaactgcgg caattgctaa ctaccctgtt agatttcatg ggttctcctc
      781 taatgtccag caccttcttt tttagtttca cgcaaaagga ctatggtgca tggagtcctt
      841 gggggcgaat ggtgcgcctc atcaaaaaaa ttgaccaact gatttacgct ttgattgctc
      901 aacgtcgggc tgagtttggc gaaaatcgtc aagatatcct tagtttatta atttcggcac
      961 gttatgacga cggacagcca atgtcagacg tagagttacg ggatgaatta atgacaatgt
     1021 tggttgcagg acatgaaacc actgcttcag ccttgacttg ggcgttttat tggattgata
     1081 gcgtaccaga agtacgtgaa aagttatttc aggaattaga tacactgaat gacgattcag
     1141 aacccagtat tattgccaaa ttgccttatt taacagcagt ttgccaagaa accctacgct
     1201 tttatcctat tgtgctgaat gctttcttcc ggcgaacaaa aaaccctatg gaaattatgg
     1261 gctacaaact gccgaaagca acattagttg ttcctagtat ttatttagcc catcatcgag
     1321 aagaagttta tccgcaatcc aagcaattcc gaccagaacg ctttttagaa aagcaattct
     1381 ccccttacga atacttaccc tttggcggcg gtaatcggcg ctgcatcggt ctagcatttg
     1441 cccaatatga aatgaaaatt gtcttagcaa caattttgtc acaattccaa gtatctagac
     1501 tcagtaagcg tccggtgcag cctgtgcgtc ggggattaac tttggcagca ccaggaggaa
     1561 tgaagatggt ggctaataaa agaatgcgta attcgtaact gcaggcatgc aagcttgcgg
     1621 ccgcgtcgtg actgggaaaa ccctggcgac tagtcttgga ctcctgttga tagatccagt
     1681 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
     1741 tattggtgag aatccagggg tccccaataa ttacgattta aatttgtgtc tcaaaatctc
     1801 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     1861 ataaacagta atacaagggg tgttatgagc catattcagc gtgaaacgag ctgtagccgt
     1921 ccgcgtctga acagcaacat ggatgcggat ctgtatggct ataaatgggc gcgtgataac
     1981 gtgggtcaga gcggcgcgac catttatcgt ctgtatggca aaccggatgc gccggaactg
     2041 tttctgaaac atggcaaagg cagcgtggcg aacgatgtga ccgatgaaat ggtgcgtctg
     2101 aactggctga ccgaatttat gccgctgccg accattaaac attttattcg caccccggat
     2161 gatgcgtggc tgctgaccac cgcgattccg ggcaaaaccg cgtttcaggt gctggaagaa
     2221 tatccggata gcggcgaaaa cattgtggat gcgctggccg tgtttctgcg tcgtctgcat
     2281 agcattccgg tgtgcaactg cccgtttaac agcgatcgtg tgtttcgtct ggcccaggcg
     2341 cagagccgta tgaacaacgg cctggtggat gcgagcgatt ttgatgatga acgtaacggc
     2401 tggccggtgg aacaggtgtg gaaagaaatg cataaactgc tgccgtttag cccggatagc
     2461 gtggtgaccc acggcgattt tagcctggat aacctgattt tcgatgaagg caaactgatt
     2521 ggctgcattg atgtgggccg tgtgggcatt gcggatcgtt atcaggatct ggccattctg
     2581 tggaactgcc tgggcgaatt tagcccgagc ctgcaaaaac gtctgtttca gaaatatggc
     2641 attgataatc cggatatgaa caaactgcaa tttcatctga tgctggatga atttttctaa
     2701 taattaattg gaccgcggtc cgcgcgttgt ccttttccgc tgcataaccc tgcttcgggg
     2761 tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc
     2821 aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg
     2881 aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg
     2941 tgctcaacgg gaatcctgct ctgcgaggct ggccgtaggc cggcctcagc ctgccgcctt
     3001 gggccgggtg atgtcgtact tgcccgccgc gaactcggtt accgtccagc ccagcgcgac
     3061 cagctccggc aacgcctcgc gcacccgctg gcggcgcttg cgcatggtcg aaccactggc
     3121 ctctgacggc cagacatagc cgcacaaggt atctatggaa gccttgccgg ttttgccggg
     3181 gtcgatccag ccacacagcc gctggtgcag caggcgggcg gtttcgctgt ccagcgcccg
     3241 cacctcgtcc atgctgatgc gcacatgctg gccgccaccc atgacggcct gcgcgatcaa
     3301 ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg ctggcgtact ccgacagcag
     3361 ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg gataccttcc aaaggcgctc
     3421 gatgcagtcc tgtatgtgct tgagcgcccc accactatcg acctctgccc cgatttcctt
     3481 tgccagcgcc cgatagctac ctttgaccac catggcatca gcggtgacgg cctcccactt
     3541 gggttccagg aacagccgga gctgccgtcc gccttcggtc ttgggttccg ggccaagcac
     3601 taggccatta ggcccagcca tggccaccag cccttgcagg atgcgcagat catcagcgcc
     3661 cagcggctcc gggccgctga actcgatccg cttgccgtcg ccgtagtcat acgtcacgtc
     3721 cagcttgctg cgcttgcgct cgccccgctt gagggcacgg aacaggccgg gggccagaca
     3781 gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg ttcttaggct tcaccacggg
     3841 gcaccccctt gctcttgcgc tgcctctcca gcacggcggg cttgagcacc ccgccgtcat
     3901 gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt ggccttgctc acaccgaagc
     3961 ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc ctcggcctcg gcgctggtca
     4021 tgctcgacag gtaggactgc cagcggatgt tatcgaccag taccgagctg ccccggctgg
     4081 cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt gctggcatgg tgcaggaaca
     4141 cgatagagca cccggtatcg gcggcgatgg cctccatgcg accgatgacc tgggccatgg
     4201 ggccgctggc gttttcttcc tcgatgtgga accggcgcag cgtgtccagc accatcaggc
     4261 ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc cggggccatg atgttgggca
     4321 ggctgccgat cagcggctgg atcagcaggc cgtcagccac ggcttgccgt tcctcggcgc
     4381 tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc ggtgggcggg tcttcggcgg
     4441 gcaggtagat caccgggccg gtgggcagtt cgcccacctc cagcagatcc ggcccgcctg
     4501 caatctgtgc ggccagttgc agggccagca tggatttacc ggcaccaccg ggcgacacca
     4561 gcgccccgac cgtaccggcc accatgttgg gcaaaacgta gtccagcggt ggcggcgctg
     4621 ctgcgaacgc ctccagaata ttgataggct tatgggtagc cattgattgc ctcctttgca
     4681 ggcagttggt ggttaggcgc tggcggggtc actacccccg ccctgcgccg ctctgagttc
     4741 ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc agccagaact tgcgctgacg
     4801 catccctttg gccttcatgc gctcggcata tcgcgcttgg cgtacagcgt cagggctggc
     4861 cagcaggtcg ccggtctgct tgtccttttg gtctttcata tcagtcaccg agaaacttgc
     4921 cggggccgaa aggcttgtct tcgcggaaca aggacaaggt gcagccgtca aggttaaggc
     4981 tggccatatc agcgactgaa aagcggccag cctcggcctt gtttgacgta taaccaaagc
     5041 caccgggcaa ccaatagccc ttgtcacttt tgatcaggta gaccgaccct gaagcgcttt
     5101 tttcgtattc cataaaaccc ccttctgtgc gtgagtactc atagtataac aggcgtgagt
     5161 accaacgcaa gcactacatg ctgaaatctg gcccgcccct gtccatgcct cgctggcggg
     5221 gtgccggtgc ccgtgccagc tcggcccgcg caagctggac gctgggcaga cccatgacct
     5281 tgctgacggt gcgctcgatg taatccgctt cgtggccggg cttgcgctct gccagcgctg
     5341 ggctggcctc ggccatggcc ttgccgattt cctcggcact gcggccccgg ctggccagct
     5401 tctgcgcggc gataaagtcg cacttgctga ggtcatcacc gaagcgcttg accagcccgg
     5461 ccatctcgct gcggtactcg tccagcgccg tgcgccggtg gcggctaagc tgccgctcgg
     5521 gcagttcgag gctggccagc ctgcgggcct tctcctgctg ccgctgggcc tgctcgatct
     5581 gctggccagc ctgctgcacc agcgccgggc cagcggtggc ggtcttgccc ttggattcac
     5641 gcagcagcac ccacggctga taaccggcgc gggtggtgtg cttgtccttg cggttggtga
     5701 agcccgccaa gcggccatag tggcggctgt cggcgctggc cgggtcggcg tcgtactcgc
     5761 tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc tgcggcgtcg gccaccttga
     5821 cccatgcctg atagttcttc gggctggttt ccactaccag ggcaggctcc cggccctcgg
     5881 ctttcatgtc atccaggtca aactcgctga ggtcgtccac cagcaccaga ccatgccgct
     5941 cctgctcggc gggcctgata tacacgtcat tgccctgggc attcatccgc ttgagccatg
     6001 gcgtgttctg gagcacttcg gcggctgacc attcccggtt catcatctgg ccggtggtgg
     6061 cgtccctgac gccgatatcg aagcgctcac agcccatggc cttgagctgt cggcctatgg
     6121 cctgcaaagt cctgtcgttc ttcatcgggc caccaagcga ttcccacaca ttatacgagc
     6181 cggaagcata aagtgtagct agatccgaag gatgagccgg gctgaatgat cgaccgagac
     6241 aggccctgcg gggctgcaca cgcgccccca cccttcgggt agggggaaag gccgctaaag
     6301 cggctaaaag cgctccagcg tatttctgcg gggtttggtg tggggtttag cgggctttgc
     6361 ccgcctttcc ccctgccgcg cagcggtggg gcggtgtgta gcctagcgca gcgaatagac
     6421 cagctatccg gcctctggcc gggcatattg ggcaagggca gcagcgcccc acaagggcgc
     6481 tgataaccgc gcctagtgga ttattcttag ataatcatgg atggattttt ccaacacccc
     6541 gccagccccc gcccctgctg ggtttgcagg tttgggggcg tgacagttat tgcaggggtt
     6601 cgtgacagtt attgcagggg ggcgtgacag ttattgcagg ggttcgtgac agttagggcg
     6661 cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     6721 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcct
//
