LOCUS       sequence-368587-        7814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human mitochondrial DNA ND1 target TALE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Mok YG, Lee JM, Chung E, Lee J, Lim K, Cho SI, Kim JS
  TITLE     Base editing in human cells with monomeric DddA-TALE fusion 
            deaminases.
  JOURNAL   Nat Commun. 2022 Jul 12;13(1):4038. doi: 10.1038/s41467-022-31745-y.
  PUBMED    35821233
REFERENCE   2  (bases 1 to 7814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-12"; volume: "13(1):4038. doi"; pages: " 
            10.1038/s41467-022-31745-y"
COMMENT     Sequence Label: pCMV-ND1 Left -GSVG-UGI
FEATURES             Location/Qualifiers
     source          1..7814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             997..1023
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1027..1053
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1057..1083
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3544..3795
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     rep_origin      4046..4474
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4133..4152)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4343..4364
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4483..4503)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4488..4817
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4668..4803
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4730..4749
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4865..4912
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4931..5329
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5487..5608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5524..5543)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5578..5597
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5657..5673)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5657..5673)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5670..5692)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5681..5697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5705..5711,5712..5729,5730..5735))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5750..5771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5888..5905)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6059..6647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6139..6158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6818..7609,7610..7678))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7441..7460
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7679..7783)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 accatggccc tgtcccgtgc ggtttgtggc acctcccgtc aactggctcc ggttctgggt
      961 tatctgggtt cccgtcaaaa acactccctg ccggactacc cgtatgatgt tccggattac
     1021 gctggctacc catacgacgt cccagactac gctggctacc catacgacgt cccagactac
     1081 gctatggaca tcgcggatct acgcacgctc ggctacagcc agcagcaaca ggagaagatc
     1141 aaaccgaagg ttcgttcgac agtggcgcag caccacgagg cactggtcgg ccatgggttt
     1201 acacacgcgc acatcgttgc gctcagccaa cacccggcag cgttagggac cgtcgctgtc
     1261 aagtatcagg acatgatcgc agcgttgcca gaggcgacac acgaagcgat cgttggcgtc
     1321 ggcaaacagt ggtccggcgc acgcgctctg gaggccttgc tcacggtggc gggagagttg
     1381 agaggtccac cgttacagtt ggacacaggc caacttctca agattgcaaa acgtggcggc
     1441 gtgaccgcag tggaggcagt gcatgcatgg cgcaatgcac tgacgggtgc ccccctgaac
     1501 ctcactcctg atcaagtcgt cgctattgca tcacacgatg gtggaaagca ggctctcgag
     1561 acagtccaac ggctgttgcc tgttctgtgc caagcacatg gattgacccc agatcaagtt
     1621 gtggccatcg caagtaacgg aggcgggaag caagcacttg aaaccgtcca aagactgttg
     1681 cctgtccttt gccaggatca cggcctcaca cccgcccagg ttgttgctat tgcaagcaac
     1741 atcggaggga aacaagcttt ggaaaccgtg cagcgccttt tgccagttct ctgtcaagat
     1801 cacgggttga caccagatca ggtggtggcc attgcaagca ataatggtgg caagcaagcc
     1861 ctcgaaaccg tccaaaggct ccttcctgtc ttgtgtcagg cacatggcct cactccagac
     1921 caggtggtgg caatcgcttc tcatgatgga ggaaaacaag cacttgagac tgttcagcgg
     1981 cttttgcctg ttctttgcca agaccatgga cttaccccag accaggtcgt tgccatcgcc
     2041 tcacatgacg gtggaaagca ggccttggaa acagtgcaac gcttgctccc cgttctgtgc
     2101 caagctcatg gtttgacacc agcacaggtt gtcgccatcg ctagcaatgg gggtggcaaa
     2161 caggcacttg aaacagtgca acggttgttg cccgtcttgt gtcaggacca tgggcttacc
     2221 cctgcacagg tcgtggcaat tgcctccaac atcgggggga agcaagccct tgaaactgtc
     2281 cagcgcctgt tgccagttct ttgtcaagcc catggattga ctcccgacca ggtggtcgca
     2341 attgcaagta acaacggtgg gaaacaggct ttggagactg tgcagagact tcttcccgtg
     2401 ttgtgccaag cacatggcct caccccagct caggttgttg ctattgcatc tcacgacggc
     2461 ggaaagcaag ctctcgagac agtccagagg ttgcttcctg tcctttgcca ggaccacggt
     2521 cttacacctg accaagttgt cgccatcgcc tcccatgatg gtgggaaaca ggccctggaa
     2581 actgtgcagc gccttctgcc tgtcctctgt caggcccacg gattgacacc cgagcaggtg
     2641 gtcgccattg cttctaacaa cgggggcaag caggcacttg agactgtcca gaggctcctc
     2701 cccgttcttt gtcaagcaca tggcctgact ccagcccaag ttgtcgccat tgccagtaat
     2761 ggtggtggta agcaggccct ggagactgtg caaaggcttc tgccagtttt gtgccaagct
     2821 catggtctga ctccagccca agttgtcgcc attgccagta atggtggtgg taagcaggcc
     2881 ctggagactg tgcaaaggct tctgccagtt ttgtgccaag cacacggtct gactccggaa
     2941 caggtggtgg cgattgcaag caacggcggc ggcaaacagg ctctagagag cattgttgcc
     3001 cagctctcca gacctgatcc ggcgctagcc gcgttgacaa atgatcatct tgttgctctt
     3061 gcttgtcttg gcggacgacc tgccctggat gctgtgaaaa aggggttggg gggatccggc
     3121 agctacgccc tgggtccgta tcagattagc gccccgcagc tgccagcata caatggtcag
     3181 accgtgggta ccttctacta tgtgaacgac gctggcggtc tggagggcaa ggtgtttagc
     3241 agcggcggtc caaccccgta cccaaactat gccaatgccg gtcatgtgga gagtcagagc
     3301 gccctgttca tgcgtgataa cggcatcagc gagggtctgg tgttccacaa caacccggaa
     3361 ggcacctgcg gtttttgcgt gaacatgacc gagaccctgc tgccggaaaa cgcgaaaatg
     3421 accgtggtgc cgccggaagg tgtcattcca gtgaagcgcg gcgctaccgg tgaaaccaaa
     3481 gtgtttaccg gtaacagcaa cggcccgaag agcccgacca aaggcggttg ctctggcggc
     3541 tcaactaatc tgagcgacat cattgagaag gagactggga aacagctggt cattcaggag
     3601 tccatcctga tgctgcctga ggaggtggag gaagtgatcg gcaacaagcc agagtctgac
     3661 atcctggtgc acaccgccta cgacgagtcc acagatgaga atgtgatgct gctgacctct
     3721 gacgcccccg agtataagcc ttgggccctg gtcatccagg attctaacgg cgagaataag
     3781 atcaagatgc tgtgataaac cacgatcgtt atgctgatca taccctaatg atcccagcaa
     3841 gataatgtcc tgtcttctaa gatgtgcatc aagcctggta catactgaaa accctataag
     3901 gtcctggata atttttgttt gattattcat tgaagaaaca tttattttcc aattgtgtga
     3961 agtttttgac tgttaataaa agaatctgtc aaccatcaaa cttctgaggc ggaaagaacc
     4021 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4081 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4141 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4201 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4261 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4321 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4381 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4441 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     4501 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4561 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4621 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4681 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4741 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4801 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     4861 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     4921 gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     4981 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     5041 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     5101 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     5161 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     5221 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     5281 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg
     5341 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     5401 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     5461 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     5521 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5581 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     5641 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5701 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     5761 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5821 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5881 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5941 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6001 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6061 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6121 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6181 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6241 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6301 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6361 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6421 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6481 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6541 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6601 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6661 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6721 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6781 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6841 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6901 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6961 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7021 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7081 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7141 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7201 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7261 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7321 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7381 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7441 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7501 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7561 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7621 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7681 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7741 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7801 tgccacctga cgtc
//
