LOCUS       sequence-368843-        2765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 donor DE - C-tag, mTagBFP2 blue fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: DE_mTagBFP2
FEATURES             Location/Qualifiers
     source          1..2765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(273..861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(353..372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(952..1743)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             join(2191..2765,1..127)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga cgtacgtcga
       61 gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct
      121 taattaatga gcttcgagac caccaggata catagattac cacaactccg agcccttcca
      181 cccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggca
      241 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      301 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      361 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      421 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      481 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      541 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      601 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      661 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
      721 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      781 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
      841 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      901 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtgatt attatttgcc
      961 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg
     1021 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg
     1081 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat
     1141 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc
     1201 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag
     1261 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct
     1321 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa
     1381 gataccagca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg
     1441 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat
     1501 ctcactctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt
     1561 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag
     1621 gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg
     1681 ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct
     1741 catgcgaaac gatcctcatc ctgtctcttg atcagatatt gatcccctgc gccatcagat
     1801 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     1861 cgccccagct ggcaattccg gttcgcttgc taagcttgca tgcctgcaag tcgactctag
     1921 aggagcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
     1981 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
     2041 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
     2101 tgggctgcct gtatcgagtg gtgattttgt gccgagctca ttactcgcat ccattctcag
     2161 gctgtctcgt ctcgtctcag gtctccaggt atgagcgagc tgattaagga gaacatgcac
     2221 atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc
     2281 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
     2341 ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc
     2401 aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg
     2461 gagagagtca ccacatacga agatgggggc gtgctgaccg ctacccagga caccagcctc
     2521 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc
     2581 cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct
     2641 gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg
     2701 atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct
     2761 ggcgt
//
