LOCUS       sequence-369028-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ABEmax-SpCas9-NGX-NGC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Xu L, Zhang C, Li H, Wang P, Gao Y, Mokadam NA, Ma J, Arnold WD, Han
            R
  TITLE     Efficient precise in vivo base editing in adult dystrophic mice.
  JOURNAL   Nat Commun. 2021 Jun 17;12(1):3719. doi: 10.1038/s41467-021-23996-y.
  PUBMED    34140489
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-17"; volume: "12(1):3719. doi"; pages: " 
            10.1038/s41467-021-23996-y"
COMMENT     Sequence Label: pCMV‐ABEmaxNGX‐NGC
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(529..1320,1321..1389))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1152..1171
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1390..1494)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1573..1592)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1764..2143
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2144..2347
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2298..2318
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2389..2408
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2389..2407
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2457..2477
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3666..7766
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKEYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7809..7829
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7838..7855
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7878..7895)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7884..8108
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8179..8195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8179..8195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8192..8214)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8203..8219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8227..8233,8234..8251,8252..8257))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8272..8293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8410..8427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8581..8811,1..358))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8661..8680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
       61 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      121 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      181 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      241 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      301 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      361 agatcctttg atcttttcta cggggtctga cactcagtgg aacgaaaact cacgttaagg
      421 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      481 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      541 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      601 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      661 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      721 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      781 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      841 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      901 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      961 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1021 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1081 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1141 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1201 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1261 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1321 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1381 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1441 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1501 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgatctcccg atcccctagg
     1561 gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     1621 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     1681 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     1741 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     1801 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     1861 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     1921 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     1981 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2041 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2101 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2161 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2221 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2281 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2341 cagagctggt ttagtgaacc gtcagatccg ctagagatcc gcggccgcta atacgactca
     2401 ctatagggag agccgccacc atgaaacgga cagccgacgg aagcgagttc gagtcaccaa
     2461 agaagaagcg gaaagtctct gaagtcgagt ttagccacga gtattggatg aggcacgcac
     2521 tgaccctggc aaagcgagca tgggatgaaa gagaagtccc cgtgggcgcc gtgctggtgc
     2581 acaacaatag agtgatcgga gagggatgga acaggccaat cggccgccac gaccctaccg
     2641 cacacgcaga gatcatggca ctgaggcagg gaggcctggt catgcagaat taccgcctga
     2701 tcgatgccac cctgtatgtg acactggagc catgcgtgat gtgcgcagga gcaatgatcc
     2761 acagcaggat cggaagagtg gtgttcggag cacgggacgc caagaccggc gcagcaggct
     2821 ccctgatgga tgtgctgcac caccccggca tgaaccaccg ggtggagatc acagagggaa
     2881 tcctggcaga cgagtgcgcc gccctgctga gcgatttctt tagaatgcgg agacaggaga
     2941 tcaaggccca gaagaaggca cagagctcca ccgactctgg aggatctagc ggaggatcct
     3001 ctggaagcga gacaccaggc acaagcgagt ccgccacacc agagagctcc ggcggctcct
     3061 ccggaggatc ctctgaggtg gagttttccc acgagtactg gatgagacat gccctgaccc
     3121 tggccaagag ggcacgcgat gagagggagg tgcctgtggg agccgtgctg gtgctgaaca
     3181 atagagtgat cggcgagggc tggaacagag ccatcggcct gcacgaccca acagcccatg
     3241 ccgaaattat ggccctgaga cagggcggcc tggtcatgca gaactacaga ctgattgacg
     3301 ccaccctgta cgtgacattc gagccttgcg tgatgtgcgc cggcgccatg atccactcta
     3361 ggatcggccg cgtggtgttt ggcgtgagga acgcaaaaac cggcgccgca ggctccctga
     3421 tggacgtgct gcactacccc ggcatgaatc accgcgtcga aattaccgag ggaatcctgg
     3481 cagatgaatg tgccgccctg ctgtgctatt tctttcggat gcctagacag gtgttcaatg
     3541 ctcagaagaa ggcccagagc tccaccgact ccggaggatc tagcggaggc tcctctggct
     3601 ctgagacacc tggcacaagc gagagcgcaa cacctgaaag cagcgggggc agcagcgggg
     3661 ggtcagacaa gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg
     3721 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc
     3781 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg
     3841 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct
     3901 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca
     3961 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg
     4021 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa
     4081 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc
     4141 acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg
     4201 acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc
     4261 ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca
     4321 gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa
     4381 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
     4441 aggataccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg
     4501 cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca
     4561 tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct
     4621 ctatgatcaa gctgtacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc
     4681 ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg
     4741 ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc
     4801 tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc
     4861 ggaagcagcg gaccttcgac aacggcatca tcccccacca gatccacctg ggagagctgc
     4921 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga
     4981 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca
     5041 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgaga
     5101 aggtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata
     5161 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg
     5221 tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc
     5281 tgagcggcga ccagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga
     5341 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa
     5401 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa
     5461 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg
     5521 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg
     5581 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg
     5641 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc
     5701 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg
     5761 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc
     5821 tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga
     5881 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg
     5941 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga
     6001 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc
     6061 ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc
     6121 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc
     6181 atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa
     6241 gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga
     6301 agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc
     6361 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac
     6421 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga
     6481 acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt
     6541 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca
     6601 actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa
     6661 agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga
     6721 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca
     6781 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc
     6841 ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt
     6901 ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg
     6961 tgcagacagg cggcttcagc aaagagtcta tccggcccaa gaggaacagc gataagctga
     7021 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgtcagc cccaccgtgg
     7081 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg
     7141 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg
     7201 actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta
     7261 agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct gcccgctttc
     7321 tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca
     7381 gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg
     7441 aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag
     7501 tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata
     7561 agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag
     7621 cccctcgggc cttcaagtac tttgacacca ccatcgaccg gaaggagtac cggagcacca
     7681 aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga
     7741 tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc gacggcagcg
     7801 aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc accattgagt
     7861 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     7921 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     7981 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     8041 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     8101 ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga cctctagcta
     8161 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8221 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag
     8281 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8341 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8401 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8461 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8521 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8581 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8641 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8701 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8761 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg t
//
