LOCUS       sequence-369074-        1905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of genetically encoded calcium indicator 
            SCW-HHL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Gohil K, Wu SY, Takahashi-Yamashiro K, Shen Y, Campbell RE
  TITLE     Biosensor Optimization Using a Forster Resonance Energy Transfer 
            Pair Based on mScarlet Red Fluorescent Protein and an 
            mScarlet-Derived Green Fluorescent Protein.
  JOURNAL   ACS Sens. 2023 Feb 24;8(2):587-597. doi: 10.1021/acssensors.2c01730.
            Epub 2023 Jan 24.
  PUBMED    36693235
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.2c01730"; journalName: "ACS Sens"; date: 
            "2023-02-24- 24"; volume: "8"; issue: "2"; pages: "587-597"
COMMENT     Sequence Label: pBAD-SCW-HHL
FEATURES             Location/Qualifiers
     source          1..1905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1159..1215
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
      181 atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc
      241 cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc
      361 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
      421 acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc
      481 gacattcata tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc
      541 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg
      601 gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc
      661 cgccaccacc taggcggcgc atgcctgccg gaccaactga ctgaagagca gatcgcagaa
      721 tttaaagagg ctttctccct atttgacaag gacggggatg ggacaataac aaccaaggag
      781 ctggggacgg tgatgcggtc tctggggcag aaccccacag aagcagagct gcaggacatg
      841 atcaatgaag tagatgccga cggtgacggc acaatcgact tccctgagtt cctgacaatg
      901 atggcaagaa aaatgaaata cagggacacg gaagaagaaa ttagagaagc gttcggtgtg
      961 tttgataagg atggcaatgg ctacatcagt gcagcagagc ttcgccacgt gatgacaaac
     1021 cttggagaga agttaacaga tgaagaggtt gatgaaatga tcagggaagc agacatcgat
     1081 ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa gggtggcgga
     1141 ggttctgtcg actcatcacg tcgtaagtgg aataagacag gtcacgcagt cagagctata
     1201 ggtcggctga gctcactaga gggcgaggca ttgatcaagg agtacatgcg gttcaaggtg
     1261 cacatggagg gctccatgga cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1321 ccntacgagg gcacccatac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     1381 tcctgggaca tcctgtcccc tcagttcggg tacggctcca gggccttcat caagcacccc
     1441 gccgacatcc ccgactacta taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg
     1501 atgaacttcg aggacggcgg cgccgtgacc gtgacccagg acacctccct ggaggacggc
     1561 accctgatcc acaaggtgaa gctccgcggc accaacttcc ctcctgacgg ccccgtaatg
     1621 cagaggaaga caatgggctg ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg
     1681 ctgaagggcg acattcatat ggccctgcgc ctgaaggacg gcggccgcta cctggcggac
     1741 tgcaagacca cctacaaggc caagaagccc gtgcagatgc ccggcgccta caacgtcgac
     1801 cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca gtacgaacgc
     1861 tcagagggcc gccaccacct aggcggcatg gacgagctgt acaag
//
