LOCUS       sequence-369082-        1941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of genetically encoded calcium indicator RCC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Gohil K, Wu SY, Takahashi-Yamashiro K, Shen Y, Campbell RE
  TITLE     Biosensor Optimization Using a Forster Resonance Energy Transfer 
            Pair Based on mScarlet Red Fluorescent Protein and an 
            mScarlet-Derived Green Fluorescent Protein.
  JOURNAL   ACS Sens. 2023 Feb 24;8(2):587-597. doi: 10.1021/acssensors.2c01730.
            Epub 2023 Jan 24.
  PUBMED    36693235
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.2c01730"; journalName: "ACS Sens"; date: 
            "2023-02-24- 24"; volume: "8"; issue: "2"; pages: "587-597"
COMMENT     Sequence Label: pcDNA-RCC
FEATURES             Location/Qualifiers
     source          1..1941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1174..1230
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(1270..1291)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1878..1899
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgtcta agggcgaaga gctgatcaag gaaaatatgc gtatgaaggt ggtcatggaa
       61 ggttcggtca acggccacca attcaaatgc acaggtgaag gagaaggcag accgtacgag
      121 ggagtgcaaa ccatgaggat caaagtcatc gagggaggac ccctgccatt tgcctttgac
      181 attcttgcca cgtcgttcat gtatggcagc cgtaccttta tcaagtaccc ggccgacatc
      241 cctgatttct ttaaacagtc ctttcctgag ggttttactt gggaaagagt tacgagatac
      301 gaagatggtg gagtcgtcac cgtcacgcag gacaccagcc ttgaggatgg cgagcttgtc
      361 tacaacgtca aggtcagagg ggtaaacttt ccctccaatg gtcccgtgat gcagaagaag
      421 accaagggtt gggagcctaa tacagagatg atgtatccag cagatggtgg tctgagagga
      481 tacactgaca tcgcactgaa agttgatggt ggtggccatc tgcactgcaa cttcgtgaca
      541 acttacaggt caaaaaagac cgtcgggaac atcaagatgc ccggtgtcca tgccgttgat
      601 caccgcctgg aaaggatcga ggagagtgac aatgaaacct acgtagtgca aagagaagtg
      661 gcagttgcca aatacagcaa ccttggtggt ggcgcatgcc tgccggacca actgactgaa
      721 gagcagatcg cagaatttaa agaggctttc tccctatttg acaaggacgg ggatgggaca
      781 ataacaacca aggagctggg gacggtgatg cggtctctgg ggcagaaccc cacagaagca
      841 gagctgcagg acatgatcaa tgaagtagat gccgacggtg acggcacaat cgacttccct
      901 gagttcctga caatgatggc aagaaaaatg aaatacaggg acacggaaga agaaattaga
      961 gaagcgttcg gtgtgtttga taaggatggc aatggctaca tcagtgcagc agagcttcgc
     1021 cacgtgatga caaaccttgg agagaagtta acagatgaag aggttgatga aatgatcagg
     1081 gaagcagaca tcgatgggga tggtcaggta aactacgaag agtttgtaca aatgatgaca
     1141 gcgaagggtg gcggaggttc tgtcgactca tcacgtcgta agtggaataa gacaggtcac
     1201 gcagtcagag ctataggtcg gctgagctca ctagagggcg aggagctgtt caccggggtg
     1261 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtccgcggc
     1321 gagggcgagg gcgatgccac caacggcaag ctgaccctga agttcatctg caccaccggc
     1381 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcgt ggcctgcttc
     1441 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1501 tacgtccagg agcgcaccat ctctttcaag gacgacggta cctacaagac ccgcgccgag
     1561 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1621 gaggacggca acatcctggg gcacaagctg gagtacaact tcaacagcca ctacgtctat
     1681 atcacggccg acaagcagaa gaactgcatc aaggctaact tcaagatccg ccacaacgtt
     1741 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1801 cccgtgctgc tgcccgacaa ccactacctg agccatcagt ccaagctgag caaagacccc
     1861 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gattacacat
     1921 ggcatggacg agctgtacaa g
//
