LOCUS       sequence-369161-        3754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dnap-F HR Donor Vector (T4 phage editing).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Adler BA, Hessler T, Cress BF, Lahiri A, Mutalik VK, Barrangou R, 
            Banfield J, Doudna JA
  TITLE     Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing.
  JOURNAL   Nat Microbiol. 2022 Dec;7(12):1967-1979. doi: 
            10.1038/s41564-022-01258-x. Epub 2022 Oct 31.
  PUBMED    36316451
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01258-x"; journalName: "Nat Microbiol"; date: 
            "2022-12"; volume: "7"; issue: "12"; pages: "1967-1979"
COMMENT     Sequence Label: pBA792
FEATURES             Location/Qualifiers
     source          1..3754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(182..997)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(182..997)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     905..924
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     989..1008
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3021..3683
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVLAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 agtataatat atcaagagcc taataactcg ggctataaac taaggaatat ctatgaaaga
       61 gttctacatt agcatcgaga cagtcggaaa taacattgtt gaacgttata ttgatgaaaa
      121 tggaaaggaa cgtacaggag gactttggca ggtggtgtta caaccaatta accaattctg
      181 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
      241 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
      301 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
      361 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
      421 ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc
      481 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
      541 gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
      601 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
      661 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
      721 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
      781 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
      841 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
      901 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
      961 gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     1021 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     1081 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
     1141 atcagcttgc gttttccctt gtccagatag cccagtagct gacatttagc ctaaatgaca
     1201 tttctccgga aagtgcgcac ggccacgctc ttgttgacgt tgagcttgct tttgacgttg
     1261 aacctcgcgt tgctcggtta ataatttctc accaaccgct ttaataatgg ccgccgcttt
     1321 cgcttgcatg tcacggttca gaggaccgag tgcatctaat accggtttca aacgttcgcg
     1381 taaatcgcgc gcagtttcct gtgcctggtc tgctttctta cgcatttcgc gggcgccggc
     1441 agccgcttgc agtgctggtt cgtaaccttg acggactgcc ttagttaagc gatccgcgac
     1501 tgcctcaggg gtttccacac gtttagaaat gcctaatttc tccgccagac cttgtggagc
     1561 gtaagcacgg ggttccacgg cctgaggcga aatcgtaaca tgtcctacag gcgggcgttc
     1621 aagcgcttca taaaatgctt ggatacgagt atggcgcgct ttactccctt caatcccacg
     1681 ctgaaggccc aggtccgcaa ccgcagcagc gaaggtcgtt tgatcacgtg tcatttgggc
     1741 cttattacca ataaattctt tagcactcaa gcgaccatct tgggtcaagg ggactacaaa
     1801 ggccgtcata tgtggggatg tctcatcgcg atgaatcgaa gctgttacaa tacggtcagc
     1861 gccatattta tcggctaacc atttatgggc tttctcaaag aatgctgctt gttgctcctg
     1921 tgacgcgctt ttccaccact cgggcgaagc ggtcatcaca tattctacgg caagtactgc
     1981 atctttacgg cgcttttcag gcaacagttc acgcaaacga cccatggcct cgtccgtact
     2041 actcgcggcc caatgttcat tttccggagt acgagatgca tccgcattcg gtgtttcacg
     2101 ttcacgatac gcatgtttca gcgacgcagc aacattaccc atcttggcga gtttcttaca
     2161 acgcataatt gcataagcgg ccatgcctgc ccctcccttt tggtgtccaa ccggctcgac
     2221 gggggcagcg caaggcggtg cctccggcgg gccactcaat gcttgagtat actcactaga
     2281 ctttgcttcg caaagtcgtg accgcctacg gcggctgcgc ctgcccacga gcttgaccac
     2341 agggattgcc caccggctac ccagccttcg accacatacc caccggctcc aactgcgcgg
     2401 cctgcggcct tgccctatca atttttttaa ttttctctgg ggaaaagcct ccggcctgcg
     2461 gcctgcgcgc ttcgcttgcc ggttggacac caagtggaag gcgggtcaag gctcgcgcag
     2521 cgaccgcgca gcggcttggc cttgacgcgc ctggaacgac ccaagcctat gcgagtgggg
     2581 gcagtcgaag ggcgaagccc gcccgcctgc cccccgagcc tcacggcggc gagtgcgggg
     2641 gttccaaggg ggcagcgcca ccttgggcaa ggccgaaggc cgcgcagtcg atcaacaagc
     2701 cccggagggg ccactttttg ccggaggggg agccgcgccg aaggcgtggg ggaaccccgc
     2761 aggggtgccc ttctttgggc accaaagaac tagatatagg gcgaaatgcg aaagacttaa
     2821 aaatcaacaa cttaaaaaag gggggtacgc aacagctcat tgcggcaccc cccgcaatag
     2881 ctcattgcgt aggttaaaga aaatctgtaa ttgactgcca cttttacgca acgcataatt
     2941 gttgtcgcgc tgccgaaaag ttgcagctga ttgcgcatgg tgccgcaacc gtgcggcacc
     3001 cctaccgcat ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga
     3061 acaagcccgg tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta
     3121 ttgcgaggaa acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc
     3181 agaacgccgt ggtggtcagc caaaagacac tttccaagct catcggacgt tctttgcgga
     3241 cggtccaata cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca
     3301 acggccccgg caccgtgttg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc
     3361 gcgaccagtt gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg
     3421 acgaatcgct gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc
     3481 agcaactacc gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg
     3541 aaccagacct gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc
     3601 tgccgatgcc cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac
     3661 gggtcacgct gccgcgccgg tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc
     3721 cagactatcc acctgcccga accagagact tatc
//
