LOCUS       sequence-369423-        7577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs4 from Klebsiella pneumoniae UNMC_8078.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: KpAvs4
FEATURES             Location/Qualifiers
     source          1..7577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5453..5500
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5720..6379)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6294..6313
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6380..6482)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6874..6891)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7008..7553)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7088..7107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaatatttct agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc
       61 atacgatata agttgtaatt ctcatgttag tcatgccccg cgcccaccgg aaggagctga
      121 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac
      181 ttacattaat tgcgttgcgc aggacactca ccgtagatgg actcttttaa accatcttat
      241 atgactctga gacgtctgtt aaacttatag cgattcggtc tgccctggtt gcatggcatc
      301 aatatatgga tggaagccaa gatgaatgaa aatctaaaaa aacagagcaa tccattttca
      361 acaggtggtg gtggcgttaa tttcgaaact cgtgtgcaag cagcgtttgc attatcgatt
      421 cttacaggtt caagcgtacc ttgtctctct cagagcatga gagcaaaaca gataaggttt
      481 cagaacaaat atgatggctc aaatacagat gattttgtga tcgttgctga agacagcgat
      541 ggtcaggaag gtaagttatt tgctcaaatt aaacatgaga taacactgag tgaatcccca
      601 gattcggttt ttgctgaagt tatcgccagt gcttggcagg acttcaaaag cgacaatttc
      661 aaaatggatc acgatgttat tgtgttgatt actggtccac tccagaaaac agatatccat
      721 aatacacttc caatattgga atgggcgaaa tttgccttat ctgcggaaga ttttatgaag
      781 aaggtacaaa caaaagggtt ctcaagcgaa gcaaagttga agaaaataga aaatttcaga
      841 ttgcatttaa gacacgcaag caatataagt gaaattgatg atacttttct atggtcattt
      901 ttgaagtcgt ttcatttgat ttcttatgac ttagattcta gtttttctgt tgtagctagt
      961 ttactatgct cacaaatcca aaataactca gatgaggctc cccccctagt attatcaaaa
     1021 atcatcacaa caattcaaaa ttttaatcag aatgcgggaa tactatctga agaaaatatc
     1081 ccgcatgatt taaaaacatt atttaataaa gtacacaaaa agaacattaa ggatgacatc
     1141 aggaagcttc aagaacgtgg tacttatatt tatacaggca tatctaatag aatttgtggc
     1201 atacatatta accggaacga acaacttgct gaaattgaaa aaaaatgtca ggaaagtcgt
     1261 tttgtctttg tcactggtat tcgaggtgtt ggaaaatcag gtattgtaaa agattttatt
     1321 tcaaccaaat ataaggacgt cccttttttc tacttgagaa ctgaagattt agacaaaagc
     1381 catctcaatg atgtatttac tgcacttggc atgagttgta gtttagcaga aattgctggt
     1441 gagttttctt tattaaaaga aaagattctt attattgagt cagtggaaaa agtactggaa
     1501 cttacttacc aaaatgcttt tattgactta ttacatttca tcaaaaatca gaatgggtgg
     1561 acagttattg ccacagggcg agattatgct acacagcaac taatttttaa ttatttacaa
     1621 cccagtggta ttgattattg caacgtagag atcaaagcac tggaatacca gcaattaagt
     1681 gaaatatgtc acaaaatacc agaactggaa aaattaattg aaaataaaag actaaccgac
     1741 ttattgaaag tcccattctt tattgatgtt gcggtaagag cattaaaaaa tggtgctcaa
     1801 tttcacaccg aaggaactga gagggacttc agagagatta tatggtcatc agttattgcg
     1861 aaggaaacag atagaagagg cgggatgcca gccaaaagaa agcgaacatt tattgacata
     1921 gctaaacaac gcgcaaagaa aatggtcgtg ggtattctgg atgacgcttt tgacccagaa
     1981 atcattgcaa agcttgaaga agataattta attcaacgac atgagagact gaatttaatc
     2041 agtccaagtc atgacgtttt agaagactgg gctttagata tctttattga taatgaatat
     2101 gtagaaaatt tcttagtacc caataagttt ttcgagcaga ttggtgatga gccttccatt
     2161 aatcgcgcct atagattatg gttaagcaga aacataaaag aaaataagga gattaatgat
     2221 tttatctata aagtattaac ggatgcttct attgccagct attggaaaga tgaaactata
     2281 actgcgattt tacaaagtga tgccgttaac atttttttaa aaatgttcag aacacaactt
     2341 ttatccaata aaaatttact gcttattaga gtattgttta tcttaagaat gacatgccaa
     2401 cagccgaaaa cactgtataa ccaagaacta gaaaaagaag aacaagaaat ttctaaaaca
     2461 ttatatctac agccttatgg tatatgctgg gatgccttga ttaatttcat ttttgaaaac
     2521 cagaatgaac ttgtgaacag cacatctatt catattatag aaatgttgaa ttcatgggtg
     2581 tcaattttaa atatcagtga taattatcct gatatatcca gaaaagtagg tttattggca
     2641 atatggttac ttgaaccact caaggattcg tatgctcgcg aagatgaata taaaagagaa
     2701 aaagttatca gtttattatt aaaattatcc cctactattg tagatgaatt tgatctactt
     2761 atggataaag aagtctttat tcctgatacc tcacaccgcc gattacatta tgttgaatat
     2821 ttaattaatc aatcattaat cggcttgaat gttcctatat tatgcaagta tcgaccagat
     2881 tttattgagc gcctagctcg tcacgaatgg ttacaagata atattgaaga tgatgacagc
     2941 attttttata aacagactac gggtgtagat cagtactttg gactaaacag agaatcagat
     3001 ttttttcctg ctagtggtgc taaaggtcct tttaaatatc tattacattt tcatcctcgt
     3061 aaagctttag attttataat tgacttgtgc aataaaacgg caaaaaaata ttctacttca
     3121 gaatttgcaa cccgctctag tggctttatg tctgatgaaa ctttagtcaa aaacgttgaa
     3181 cttacactca atgacggtac cgtcgttacg caattttctt ctccacactt atggaagggt
     3241 tatagaggcc attcaacgct accatatatc cttcaatgtg cattaatggc tttggagaat
     3301 tggctcattg attcactaga tagccttgaa aaaaatgaca ctattgaatg gctttttaat
     3361 tatattttac tatctagcaa ctcagtaatg ccaacatctg tattggcatc agttgctact
     3421 ggttttccag acaaaattag taaagctgct tttcctatcc taagatgccc tgagttttac
     3481 aatttagata tccaacgtct tgtcaatgaa cgaggaggaa gtgaacaaaa ctggttctcg
     3541 aatatgtttc ataacgatat catgaatgac acttatgtag aggaacgacg cattgctgca
     3601 cttcgtcctt ggagaaaaga aactctagaa agtttattga ctaggttaca gcttagcggg
     3661 aaaaacaagg ccgacacact aaaaattgtg gatagcctaa ttgcgttatc cttagagagc
     3721 aacgatgaga acttacgttt tctagtacac agagcagata ctagaacatg gtcatttact
     3781 gctgataaag agaacagccg gattattgcg caaagcaatc cggaattacc ggaggatctg
     3841 caacaaattc atgatgaaca tagtgctaca catactcaga acagtttaat taacaaatta
     3901 ttcttatggt caagaaaatc atttgaagaa aatattaata atacatactt taattctcat
     3961 acagaggctc ttaccgctgc aaaatatctt tttgataaat ttcaaaacaa tgaagttgat
     4021 aacccggtta tggtaattgg cacaatcacc aaaaccgcag cagtttgtac acgagatttg
     4081 tttgagaacc tttccgaaga tgaaaaaaac tggtgtaaaa aaataattat tgaatgcatc
     4141 attatgaatg ccgattgtat taacgggtta tcacttcatg ataagacaga tagttatggt
     4201 tctggcgctt gcgcatttgt tttacctaaa ttattccgct acgtaaaaaa tgatgaagaa
     4261 ctcaatgaat taaaatttac attagcaata gcattaaccc atgaaaatat tgatatcggt
     4321 gaatatgctg caaaaggggt aagagagttt ctatggacta cccatccgac cttggccttc
     4381 caatgcatat atggtgctac tatgtttgcg aggtttaaaa aagaaaatat taactcacaa
     4441 agattttatt tatattctga tactgaacta gctcaagcta aagcgcattg gtcttatttg
     4501 ataacctctt ttcgtgaaga gttctttaac acaaccccac cattaattga aaatatttca
     4561 ttaaaaacac attcatcttg gcacattcac cttccaatat tgatgattcc attttgcgtt
     4621 gataatcctc tgctaattaa tttatacaag aaaatagtac attttacgtt tgatgaggaa
     4681 tacaagagac ataaaagtaa tcatgacgat gaaaccatcc aacatgacat taggaagcag
     4741 atcaccaatt gcttcgctga gcattttata cacacaagaa aaactggatt cgtgccattt
     4801 aagacactgc tacttgaagg atgttcaacg gcaccacgta ttgtgtacat gactaaatta
     4861 ctattccaca tcgctatgga gaaagcagaa gacctagagt ctttatggga attctggtta
     4921 atgttagcgc cagaagcaca caaaattgcg ctaaaagatg taaatgaaca gtattctggt
     4981 atacagagtg atctgaacca attgctgcgc ggaatgttgt atgcagacac taggtggcag
     5041 ggacacgaga atgaagcacg cgatatagca tatggttcag tgcatatact agattttgca
     5101 aaaatatcag gaaataacag ccacgtattt atggcgttat catcgttgat ttatcacttt
     5161 catcatatat ttttcgacga gggtatccat atccttgctg aaaaactcat catgaatagc
     5221 aagttaattg ataagcaaat taatacagca ttttatctag aaatggctat aggacgatat
     5281 ttgcaaattg aaaatcgggg aatattgagt cgtaaaatgt ataattcctg tatgacatta
     5341 ttatcgggta ttgtcgagac aggttctgcg agagcctatt atctccggga aaatctaata
     5401 agatcacgca gagtatttac ctaaatggct tctttgtaat ggatcgcaat aactagcata
     5461 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaacctc aggcatttga
     5521 gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata
     5581 agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt
     5641 ctgccattca tccgcttatt atcacttatt caggcgtagc accaggcgtt taagggcacc
     5701 aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcaatac tgttgtaatt
     5761 catttaacat tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca
     5821 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catagtgaaa acgggggcga
     5881 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     5941 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     6001 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     6061 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     6121 tatcccatat caccagctca ccgtctttca tcgccatacg gaactctgga tgagcattca
     6181 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     6241 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     6301 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     6361 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     6421 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     6481 caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag
     6541 gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt
     6601 cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg
     6661 gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc
     6721 aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg
     6781 atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca
     6841 gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt
     6901 cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc
     6961 caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc
     7021 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca
     7081 ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct
     7141 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct
     7201 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
     7261 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca
     7321 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt
     7381 catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc
     7441 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg
     7501 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc
     7561 atcttattaa tcagata
//
