LOCUS       sequence-369428-       18141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian cell expression of 3xFLAG-HUWE1 H669Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18141)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 18141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDARMO_CMVT_3xFLAG-HUWE1_H669Q
FEATURES             Location/Qualifiers
     source          1..18141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..224
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(288..876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(368..387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(915..1706,1707..1775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1538..1557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1776..1847)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  1872..2037
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     enhancer        2131..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3140..3159
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      3212..3221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3224..3289
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3308..3331
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(16568..16588)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(16986..16997)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(17064..17081)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    17070..17277
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        join(17325..17330,17331..17347,17348..17353)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             17542..18075
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gaatatagtc tttacatgcc cttggccacg cccctcttta atacgacggg caatttgcac
       61 ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca
      121 taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt
      181 ttagatctat tttgttcagt ttaagacttt attgtccgcc cacacccgct tacgcagggc
      241 atctgcgcat ccacaggaag ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      721 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      901 gggtctgacg cttattacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1021 ttaccatctg gccccagtgc tgcaatgata ccgcgtgacc cacgctcacc ggctccagat
     1081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttaggaccca
     1861 agtcaaccgg ttgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt
     1921 ttaaggatta tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc
     1981 taatacgaaa attaaaaatg acaaaatagt ttggaactag atttcactta tctggttcag
     2041 cctgcggtcc ggattcgccc ttaagctagc tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2401 gtacatctac gtattagtca ttgctattac catggtgatg cggttttggc agtacatcaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2581 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     2641 tttagtgaac cgtcagatcc tcactctctt ccgcctcgct gtctgcgagg gccagctgtt
     2701 gggctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga tcggaaaccc
     2761 gtcggcctcc gaacggtact ccgccaccga gggacctgag cgagtccgca tcgaccggat
     2821 cggaaaacct ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg
     2881 tggcgggcgg cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt
     2941 aattaaagta ggcggtgttg acaccgcgga tggtcgaggt gaggtgtggc aggcttgaga
     3001 tccagctgtt ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg
     3061 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg
     3121 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaaagt
     3181 tggccaggcc tagttggcca ggcctagtgc cgccaccatg ggtgactaca aagaccatga
     3241 cggtgattat aaagatcatg acattgatta caaggatgac gatgacaagg gcggtggtag
     3301 cggtgggctg gaagttctgt tccaggggcc cgtcgacatg aaagtagaca ggactaaact
     3361 gaagaagaca cctactgagg ctcctgcaga ctgcagagcc ttaatagaca aactcaaagt
     3421 ttgtaatgat gagcaacttc tcttggaact gcagcagatc aaaacatgga acattggaaa
     3481 gtgcgagtta tatcactggg tggacctgtt ggaccgcttc gatggaatac tggcagatgc
     3541 tggacagaca gtggagaata tgtcatggat gctcgtatgt gataggccag aaagagagca
     3601 actgaaaatg cttctcttgg ctgtgttgaa cttcacagcc ttgctcattg agtacagctt
     3661 ttcccggcat ctgtacagtt ccatagagca tttgacaact ttattggctt cctctgatat
     3721 gcaagtggtg ctggcagtcc tcaatctcct atatgtattt agcaaaagat caaactacat
     3781 cactcgtctg ggatctgaca agaggacccc gctgctaact cggctacaac atttggcaga
     3841 gagctggggt ggaaaggaga atggctttgg acttgcagaa tgttgcagag acttgcatat
     3901 gatgaaatat ccacccagtg caactacact acactttgaa ttctatgcag atcctggggc
     3961 cgaggtcaaa attgagaaaa ggacaactag taacacacta cattatattc acatagagca
     4021 acttgacaag atttcagaaa gcccttctga aatcatggaa tctcttacca aaatgtacag
     4081 cattcctaag gataagcaga tgctgttatt tacacacata cgactggccc atggcttttc
     4141 taatcacagg aagcgattgc aggcagttca ggccagactg catgcaatat ctatattagt
     4201 gtattccaat gccttgcagg aatcagcaaa cagtatcttg tataatggct tgatagagga
     4261 gttagtggac gtgctgcaaa taacggataa gcagcttatg gagattaaag cagcttcttt
     4321 acgaacatta acatcaattg tccacttgga gagaactccc aaactcagca gtattattga
     4381 ctgtactgga actgcctcct accatggatt tttgccagtg cttgtaagga actgtatcca
     4441 ggccatgatt gatccttcca tggatccata ccctcaccag tttgccactg ctctcttctc
     4501 ttttttatac catctggcca gctacgatgc tggtggtgaa gccttggtct cctgtggaat
     4561 gatggaagcc ttattgaagg tcataaagtt tcttggcgat gaacaggacc agataacatt
     4621 tgtcaccaga gccgtcagag tggttgacct tatcaccaac ctggatatgg cagcttttca
     4681 atcccatagt ggactttcta tcttcattta tagacttgag catgaagtag atctgtgccg
     4741 aaaagaatgt ccgtttgtga tcaagccaaa gatccagaga cccaatacta cacaagaagg
     4801 agaggaaatg gaaactgata tggatggagt ccagtgtatt ccacaacgag cagcacttct
     4861 gaaatccatg ttgaatttcc tcaagaaggc catccaagac cctgctttct cagatggcat
     4921 acgacatgtg atggatggtt ctctgcctac ctccctgaaa cacatcatca gcaatgcaga
     4981 atactatggc ccatcactct tcctcctagc tactgaagtg gtgactgtgt ttgtatttca
     5041 agaaccatca ctgctctcct cactccagga caatggattg acagatgtca tgctgcatgc
     5101 actgcttatc aaagatgttc ctgctacccg tgaagtcctt ggctccctcc caaatgtatt
     5161 cagtgcactc tgtttgaatg cccgaggtct tcagtctttt gttcagtgtc agccttttga
     5221 acgcctcttc aaagttcttc tgtctccaga ttacctccca gccatgcgga ggaggagaag
     5281 ttctgatccc cttggggata ctgcatccaa cctggggagt gctgtcgatg agctcatgag
     5341 acagcagccc acccttaaaa cagatgcaac gactgccatc atcaagttac ttgaagaaat
     5401 ctgtaatctt ggaagggacc ccaaatacat ctgtcagaag ccatcgatcc agaaggcaga
     5461 tggcactgcc actgctcctc ccccaaggtc taatcatgcc gcagaagaag cctctagtga
     5521 ggatgaggag gaagaggaag tacaggccat gcagagcttt aattctaccc agcaaaatga
     5581 aactgagcct aatcagcagg ttgttggtac agaggaacgt attcctattc ccctcatgga
     5641 ttacatcctt aatgtgatga aatttgtgga atctattctg agcaacaata caacagatga
     5701 ccactgccag gaatttgtga atcagaaagg actgttgcct ttggttacca ttttgggtct
     5761 tcccaatctg cccattgact ttcccacatc tgctgcctgt caggctgttg caggtgtctg
     5821 caaatccata ttgacactgt cacatgaacc caaagtcctt caagagggtc tccttcagtt
     5881 ggactccatc ctctcctccc tggagccctt acaccgcccc attgaatccc ctgggggctc
     5941 agtgttgttg cgagaactgg cttgcgcagg caatgttgct gatgctaccc tctcagccca
     6001 ggccacacct ctgctgcatg cactcactgc tgcccatgcc tacatcatga tgtttgttca
     6061 tacttgcaga gttggacaga gtgaaattcg ttccatctcc gtaaaccagt ggggctctca
     6121 attgggtctg agtgttttga gcaagctgag ccagttatac tgttccctgg tgtgggaaag
     6181 cactgtcctc ctctctctgt gtaccccaaa cagcctacca tctgggtgtg aatttggcca
     6241 ggcagatatg cagaaactgg ttccaaagga tgagaaggca ggtacgaccc agggcggaaa
     6301 aagatcagat ggggaacagg atggagcagc tggaagtatg gatgcttcta cccagggctt
     6361 attagaaggc attgggctag atggtgacac attggctccc atggagacag atgaacctac
     6421 tgcttcagac tctaagggca aatctaaaat cacaccagca atggctgcca gaattaagca
     6481 aatcaagcct ttgttatcag cttcctccag attaggccga gcacttgctg agctatttgg
     6541 acttcttgtt aaactttgtg tgggatctcc tgtccgccag agaaggagcc atcatgctgc
     6601 cagcaccact acagcaccga cacctgccgc gcgatcaaca gcctcagctc tcactaagct
     6661 cttgactaag gggttatctt ggcagccccc accatataca cctactcccc gattcaggct
     6721 gacattcttc atctgttcag ttggtttcac atccccaatg ctgtttgatg agaggaagta
     6781 tccctaccac ctcatgctgc aaaaatttct ctgctccgga ggccacaatg ctctttttga
     6841 aactttcaac tgggctctgt ccatgggagg taaagttcct gtttctgagg gattggaaca
     6901 ctcagacttg cctgatggca caggagaatt cctagatgcc tggcttatgc tggtggagaa
     6961 gatggtgaat cccaccacgg tgcttgaatc tccacattcg ctgcctgcca aattgcctgg
     7021 aggtgtccag aactttcccc agttcagtgc actgcgcttc cttgtggtaa ctcagaaagc
     7081 agcctttact tgcatcaaaa acttatggaa ccggaaaccc ctgaaggtat atggtggacg
     7141 aatggctgaa tcgatgctgg ccattctatg ccacatcctc cgaggagaac ctgtgattcg
     7201 agagagacta agcaaggaga aggaggggtc tcgaggagaa gaggatacag ggcaagagga
     7261 aggtggctcc cgccgggaac ctcaagtcaa ccagcaacaa ctgcaacagc tcatggacat
     7321 gggcttcaca agggaacatg caatggaggc actgttgaac accagcacca tggagcaggc
     7381 cacagagtac cttttaaccc accctcctcc aatcatggga ggagttgttc gggatctcag
     7441 catgtctgaa gaggaccaga tgatgagagc aattgctatg tctctgggac aggatattcc
     7501 aatggatcaa agggcagagt cacctgagga agttgcttgc cggaaggagg aagaggaacg
     7561 gaaagctcgg gaaaagcagg aggaggaaga ggctaaatgt ctagagaagt tccaggatgc
     7621 tgacccgttg gaacaagatg agctccacac tttcacagat actatgttgc caggctgctt
     7681 ccaccttctt gatgagctgc cagacacagt ataccgtgtg tgtgacctga tcatgacagc
     7741 aatcaaacgt aatggagcag attatcgtga catgattctg aagcaagtag tcaatcaggt
     7801 gtgggaagct gctgatgtat tgatcaaagc tgctcttccc ctgacaacaa gtgacacaaa
     7861 aaccgtgtca gagtggataa gtcagatggc cacactgccc caggcctcca atttggctac
     7921 tagaatcttg cttttaacgc tactttttga ggagttgaag ctaccttgtg cttgggtggt
     7981 tgaatcaagt ggcatcctta atgtcctaat caaactcttg gaagtggttc agccctgcct
     8041 ccaggcagcc aaggagcaga aggaagtcca gaccccaaag tggatcacac cagtgttgct
     8101 cctgattgat ttctatgaaa agacagccat ctcctcaaaa aggagagccc agatgactaa
     8161 gtacctgcaa tccaacagca acaactggcg ctggtttgat gatcgctctg ggcgttggtg
     8221 tagttacagt gcaagcaaca atagcactat tgattctgcc tggaaatctg gagagacaag
     8281 cgtgcgattc actgcaggcc gaagaagata cacggtccaa ttcactacaa tggtgcaggt
     8341 taatgaggaa acagggaacc gacgccctgt gatgctgact ctcctcaggg tacctcggct
     8401 gaataaaaat tcaaaaaaca gcaatggaca ggaactagag aagacgctgg aagaaagcaa
     8461 agaaatggat atcaaacgta aagaaaataa aggcaatgat acccctttgg ccctagagag
     8521 tacaaacact gaaaaggaga caagcctgga ggaaacaaaa atcggggaga tcctgatcca
     8581 gggcttgaca gaagatatgg tgactgtttt aatccgggcc tgcgtgagca tgctgggagt
     8641 ccctgtggac ccagatactt tgcatgccac ccttcgtctc tgtctgaggc tcacccggga
     8701 ccacaaatat gccatgatgt ttgcagaact gaagagtacc cgcatgatct tgaatttgac
     8761 ccagagctca ggcttcaatg ggtttactcc cctggtcacc cttctcttaa gacacatcat
     8821 tgaggacccc tgtacccttc gtcataccat ggaaaaggtt gttcgctcag cagctacaag
     8881 tggagctggt agcactacct ctggtgttgt gtctggcagc ctcggctctc gggagatcaa
     8941 ctacatcctt cgtgtccttg ggccagccgc atgccgcaat ccagacatat tcacagaagt
     9001 ggccaactgc tgtatccgca tcgcccttcc tgcccctcga ggctcaggaa ctgcttcaga
     9061 tgatgaattt gagaatctta gaattaaagg ccctaatgct gtacagctgg tgaagaccac
     9121 ccctttgaag ccctcacctc tgcctgtcat ccctgatact atcaaggaag tgatctatga
     9181 tatgctgaat gctctggctg cataccatgc tccagaggaa gccgataaat ctgatcctaa
     9241 acctggggtt atgacccaag aggttggcca gctcctgcaa gacatgggtg atgatgtata
     9301 ccagcagtac cggtcactta cgcgtcagag cagtgacttt gatacgcagt caggtttttc
     9361 cattaatagt caggtctttg ctgcagatgg tgcctccact gagacttccg catctgggac
     9421 ctcccaagga gaggcttcaa ctccagagga gtctcgagat gggaagaaag ataaagaagg
     9481 ggaccgggcc tctgaggaag gcaaacagaa aggcaagggc agcaaacctt taatgcctac
     9541 ctccactatc cttcgtcttc tggcagagtt ggtgaggtcc tatgttggta ttgctaccct
     9601 gattgccaac tacagctaca ctgtgggcca gtctgaactg atcaaagagg actgcagtgt
     9661 gctagctttt gttctggacc acctgctccc acatacccag aatgcagaag acaaggacac
     9721 ccctgccttg gcccgcctgt tcctcgcaag cctggctgct gcagggagtg gcacagatgc
     9781 ccaggtggcc ctagtgaatg aagtaaaagc agcccttgga cgggcactgg ctatggctga
     9841 gagtacagag aaacatgcca ggcttcaggc agtgatgtgt atcatcagta ctatcatgga
     9901 gtcctgcccc tccacctcca gcttctacag cagtgccaca gcgaagaccc agcacaatgg
     9961 catgaacaac atcattcggc ttttcctgaa gaagggactg gttaatgacc tggccagagt
    10021 acctcacagc ttagacctgt ccagtcccaa catggccaac acagtcaatg ctgctctgaa
    10081 gcctttggaa acactttccc ggattgtgaa ccagcccagt agcctttttg gcagcaagag
    10141 tgcttctagc aagaacaagt ctgagcagga tgcccaagga gcctctcaag attccagtag
    10201 caaccagcag gacccaggcg agcctgggga agcagaagtg caggaggagg atcatgatgt
    10261 cactcagaca gaggtggcag atggggatat catggatggg gaggctgaaa ccgactcagt
    10321 ggtgattgct gggcagcctg aggtgctcag ttcacaagag atgcaggttg agaatgagct
    10381 ggaggacctg atagatgagt tgcttgagag ggatggcgga tctgggaaca gtacaattat
    10441 agtgagcaga agtggagagg atgaatcaca agaggacgtg ctgatggatg aagctccttc
    10501 caacctcagc caagcttcca ccttgcaggc caaccgagaa gattccatga atatcctgga
    10561 ccctgaggat gaggaggagc acactcagga agaggacagc agtggcagta acgaggatga
    10621 ggatgatagt caggatgaag aggaggagga ggaggaagat gaggaagatg atcaggagga
    10681 tgatgaaggt gaagagggag atgaagacga tgacgacgat ggctctgaga tggaattgga
    10741 tgaggattat cctgatatga acgcttctcc cttggtccga tttgagcgct ttgaccggga
    10801 ggatgatctc atcattgagt ttgacaacat gttctccagt gctacagaca tccccccatc
    10861 cccaggaaat atccctacca cccatccact gatggtgcgc catgcagacc acagttctct
    10921 gacactgggc agtggctctt caacaactcg tctcacccag ggcatcgggc gcagtcagag
    10981 gaccctaagg cagctgacgg ccaatactgg ccacaccatt catgttcact accctgggaa
    11041 tcgccagccc aaccctcctc ttatactgca gaggttgctt ggtccctcag ctgctgctga
    11101 catccttcag ctgagcagca gccttcccct acaaagccgg ggtcgggccc gcctcctggt
    11161 aggcaacgat gacgtccaca tcatcgcccg ttctgatgat gagctgctgg atgacttttt
    11221 ccatgatcag agcacagcta ccagccaagc aggaaccctg tccagcatcc ccacagccct
    11281 gacccgctgg acagaagaat gcaaagttct cgatgctgag agcatgcatg actgtgtttc
    11341 agtggttaaa gtgtccattg tcaatcacct ggaattcctg agggatgagg agctggaaga
    11401 aaggcgagag aagcgcagga aacaactggc tgaggaagaa acaaagataa ctgataaagg
    11461 caaagaagat aaggagaaca gggatcagag tgcacagtgt actgcatcta agtcaaatga
    11521 ctccactgaa cagaatctct cagatgggac gcctatgcct gacagctacc caacaacccc
    11581 atcttcaact gatgcagcta catctgagtc caaggagacc cttggcactc tgcaatcctc
    11641 acaacagcaa ccaacactcc caaccccacc agctttggga gaggttcctc aggagctgca
    11701 gtctccagct ggagaagggg gcagctctac acagctattg atgcctgtag agccagagga
    11761 attgggtccc acaaggccaa gtggggaagc agaaacaact cagatggagt tatccccagc
    11821 tcccactata acctcacttt ccccagagag agctgaggat tctgatgcac tgacggctgt
    11881 cagcagtcag ctagaaggct ctcctatgga tacaagcagc ctggcttcct gtaccttaga
    11941 ggaggctgtg ggtgacactt cagcagctgg cagttctgag cagcccagag caggcagctc
    12001 cactcctggg gatgccccac cagctgtggc ggaagtgcaa ggcaggagtg atgggtcagg
    12061 ggaatctgcc cagccacctg aggacagctc cccacctgca tcctctgaga gctcttccac
    12121 cagagattct gccgtggcca tttctggcgc cgattcccga ggaatcctag aagagccgtt
    12181 gccttcaaca agcagtgaag aagaagatcc ccttgcgggt atcagtctcc ctgaaggtgt
    12241 ggacccctct tttctggctg ccctgcctga tgacatccgt cgggaagttc tacagaacca
    12301 gctaggcatt cgtccaccaa cccggactgc cccctccaca aatagctcag cgcctgcagt
    12361 ggtggggaat cctggtgtga ctgaggtgag tcccgagttt cttgcggcac tgccacccgc
    12421 gatccaagag gaagtactgg cacagcagag agctgagcag cagcgacgag aactagcaca
    12481 gaatgccagc tcagacaccc ctatggaccc tgtgaccttc atccagactc tgccctcaga
    12541 cctgcgccgt agtgtcctag aggatatgga ggacagtgtg ttagctgtga tgccacctga
    12601 cattgcagct gaggctcaag ccctgagacg agagcaagaa gcccggcagc gacagctcat
    12661 gcatgagcgt ctgtttgggc acagtagcac ctccgcactc tctgctattc tccgaagccc
    12721 ggctttcacc agtcgcttaa gtggcaaccg tggggtccag tatactcgcc ttgctgtgca
    12781 gagaggtggc accttccaga tggggggtag cagcagccat aacaggcctt ctggcagtaa
    12841 tgtagatact ctcctccgcc tccgaggacg gctccttctg gaccacgaag ccctttcttg
    12901 tctcttggtc ctactttttg tggatgagcc aaagctcaat actagccgtc tacaccgagt
    12961 actgagaaat ctctgctacc atgcccagac ccgccactgg gtcatccgca gtctgctctc
    13021 catcttgcag cgcagcagtg agagtgagct atgcattgaa acacccaaac tcactacaag
    13081 tgaggaaaag ggcaaaaagt cgagcaagag ctgtgggtca agtagccatg agaaccgtcc
    13141 cctggacctg ctacacaaga tggagtcaaa gagctccaac cagctttcct ggctctcagt
    13201 atccatggat gcagccctag gctgcaggac taatatattt cagatccagc gttcaggggg
    13261 gcgtaaacat accgagaagc atgcaagcgg tggctccacc gtccacatcc atccccaagc
    13321 tgctcctgtt gtctgcagac acgttttgga tacactcatt caattggcca aggtatttcc
    13381 cagccacttc acacagcagc ggaccaaaga aacaaactgt gagagtgatc gggaaagggg
    13441 caataaggcc tgtagcccat gctcctcaca gtcctccagc agtggcattt gcacagactt
    13501 ctgggactta ttggtaaaac tggacaacat gaatgtcagc cggaaaggca agaactccgt
    13561 gaagtcagtg ccagtgagcg ctggcggtga gggggaaacc tctccataca gcctcgaggc
    13621 ctctccactg gggcagctca tgaacatgtt gtcacaccca gtcatccgcc ggagctctct
    13681 cttaactgag aaactcctca gactcctttc tctcatctca attgctctcc cagaaaacaa
    13741 ggtgtcagaa gcacaggcta attctggcag cggtgcttcc tccaccacca ctgccacctc
    13801 aaccacatct accaccacca ccactgccgc ctccaccacg cccacacccc ctactgcacc
    13861 cacccctgtc acttctgctc cagccctggt tgctgccacg gctatttcca ccattgtcgt
    13921 agctgcttcg accacagtga ctacccccac gactgctacc actactgttt caatttctcc
    13981 cactactaag ggcagcaaat ctccagcgaa ggtgagtgat gggggcagca gcagtacaga
    14041 ctttaagatg gtgtcctctg gcctcactga aaaccagcta cagctctctg tagaggtgtt
    14101 gacatcccac tcttgttctg aggaaggctt agaggatgca gccaacgtac tactgcagct
    14161 ctcccggggg gactctggga cccgggacac tgttctcaag ctgctactga atggagcccg
    14221 ccatctgggt tatacccttt gtaaacaaat aggtaccctg ctggccgagc tgcgggaata
    14281 caacctcgag cagcagcggc gagcccaatg tgaaaccctc tctcctgatg gcctgcctga
    14341 ggagcagcca cagaccacca agctgaaggg caaaatgcag agcaggtttg acatggctga
    14401 gaatgtggta attgtggcat ctcagaagcg acctttgggt ggccgggagc tccagctgcc
    14461 ttctatgtcc atgttgacat ccaagacatc tacccagaag ttcttcttga gggtactaca
    14521 ggtcatcatc cagctccggg acgacacgcg ccgggctaac aagaaagcca agcagacagg
    14581 caggctaggt tcctccggtt taggctcagc tagcagcatc caggcagctg ttcggcagct
    14641 ggaggctgag gctgatgcca ttatacaaat ggtacgtgag ggtcaaaggg cgcggagaca
    14701 gcaacaagca gcaacgtcgg agtctagcca gtcagaggcg tctgtccgga gggaggaatc
    14761 acccatggat gtggaccagc catctcccag tgctcaagat actcaatcca ttgcctccga
    14821 tggaacccca cagggggaga aggaaaagga agaaagacca cctgagttac ccctgctcag
    14881 cgagcagctg agtttggacg agctgtggga catgcttggg gagtgtctaa aggaactaga
    14941 ggaatcccat gaccagcatg cggtgctagt gctacagcct gctgtcgagg ccttctttct
    15001 ggtccatgcc acagagcggg agagcaagcc tcctgtccga gacacccgtg agagccagct
    15061 ggcacacatc aaggacgagc ctcctccact ctcccctgcc cccttaaccc cagccacgcc
    15121 ttcctccctt gacccattct tctcccggga gccctcatct atgcacatct cctcaagcct
    15181 gccccctgac acacagaagt tccttcgctt tgcagagact caccgcactg tgttaaacca
    15241 gatcctacgg cagtccacga cccaccttgc tgatgggcct tttgctgtcc tggtagacta
    15301 cattcgtgtc ctcgactttg atgtcaagcg caaatatttc cgccaagagc tggagcgttt
    15361 agatgagggg ctccggaaag aagacatggc tgtgcatgtc cgtcgtgacc atgtgtttga
    15421 agactcctat cgtgagctgc atcgcaaatc ccccgaagaa atgaagaatc gattgtatat
    15481 agtatttgaa ggagaagaag ggcaggatgc tggcgggctc ctgcgggagt ggtatatgat
    15541 catctctcga gagatgttta accctatgta tgccttgttc cgtacctcac ctggtgatcg
    15601 agtcacctac accatcaatc catcttccca ctgcaacccc aaccatctgt cctattttaa
    15661 atttgtcgga cgcattgtgg ccaaagctgt atatgacaac cgtcttctgg agtgctactt
    15721 tactcgatcc ttttacaaac acatcttggg caagtcagtc agatatacag atatggagag
    15781 tgaagattac cacttctacc aaggtctggt ttatctgctg gaaaatgatg tctccacact
    15841 aggctatgac ctcaccttca gcactgaggt ccaagagttt ggagtttgtg aagttcgtga
    15901 cctcaaaccc aatggggcca acatcttggt aacagaggag aataagaagg agtatgtaca
    15961 cctggtatgc cagatgagaa tgacaggagc catccgcaag cagttggcgg ctttcttaga
    16021 aggcttctat gagatcattc caaagcgcct catttccatc ttcactgagc aggagttaga
    16081 gctgcttata tcaggactgc ccaccattga catcgatgat ctgaaatcca acactgaata
    16141 ccacaagtac cagtccaact ctattcagat ccagtggttc tggagagcat tgcgttcttt
    16201 cgatcaagct gaccgtgcca agttcctcca gtttgtcacg ggtacttcca aggtacccct
    16261 gcaaggcttt gctgccctcg aaggcatgaa tggcattcag aagtttcaga tccatcgaga
    16321 tgacaggtcc acagatcgcc tgccttcagc tcacacatgt tttaatcagc tggatctgcc
    16381 tgcctatgag agctttgaga agctccgcca catgctactg ttggctatcc aggagtgctc
    16441 tgaaggcttt gggctggcct aataagcggc cgctgatgat aatggcctgt ggccgtaatg
    16501 gcctgtggcc gtaaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
    16561 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
    16621 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
    16681 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
    16741 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
    16801 ctttccccct ccctattgcc acggcggaac tcagtgccgc ctgccttgcc cgctgctgga
    16861 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
    16921 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
    16981 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
    17041 ctcttccgcg gcttcgagat ctgcctcgac tgtgccttct agttgccagc catctgttgt
    17101 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
    17161 ataaaatgag gaaattgcat caaattgtct gagtaggtgt cattctattc tggggggtgg
    17221 ggtggggcag gacagcaagg gggaggattg ggcagacaat agcaggcatg ctggggactc
    17281 gagttaaggg cgaatcgctg ggacgcccga acggtggcta ctcgttgaca taagcctgtt
    17341 cggttcgtaa actgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
    17401 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
    17461 ttgtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
    17521 tgatgttatg gagcagcaac gatgttacgc agcagcaacg atgttacgca gcagggcagt
    17581 cgccctaaaa caaagttagg tggctcaagt atgggcatca ttcgtacatg taggctcggc
    17641 cctgaccaag tcaaatccat gcgggctgct cttgatcttt tcggtcgtga gttcggtgat
    17701 gtagccacct actcccaaca tcagccggac tccgattacc tcgggaactt gctccgtagt
    17761 aagacattta ttgcgcttgc tgccttcgac caagaagcgg ttgttggcgc tctcgcggct
    17821 tacgttctgc ccaagtttga gcagccgcgt agtgagatct atatctatga tctcgcagtc
    17881 tccggcgagc accggaggca gggcattgcc accgcgctca tcaatctcct caagcatgag
    17941 gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag attacggtga cgatcccgca
    18001 gtggctctct atacaaagtt gggcatacgg gaagaagtga tgcactttga tatcgaccca
    18061 agtaccgcca cctaacaatt cgttcaagcc gagatcggct tcccggccgc ggagttgttc
    18121 ggtaaattgt cacaacgccg c
//
