LOCUS       sequence-369727-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL9 and ConRE connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT074
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(293..1084,1085..1153))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     916..935
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1154..1258)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1361..1949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1850..1869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agacgtgtga ctagtctctc tgcagggacc agcatattca tgtcagcgaa atctgctcgt
       61 cagtggtgct cacactgacg aatcatgtac agatcatacc gatgactgcc tggcgactca
      121 caactaagca agacagccgg aaccagcgcc ggcgaacacc actgcatata tggcatatca
      181 caacagtcca gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      241 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      301 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
      361 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
      421 caatgatacc gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
      481 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
      541 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
      601 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
      661 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
      721 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
      781 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
      841 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
      901 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
      961 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1021 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1081 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1141 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1201 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1261 catttccccg aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1321 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1381 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1441 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1501 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1561 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1621 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1681 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1741 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1801 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1861 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1921 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1981 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2041 ataaccgtgc ggccgcagca cacgctgtcc gcaggttgga gcaatagact gataatctgg
     2101 gatagtaagg gaggctgact tttcgggtaa acttctgttc gtaactgtat tcgccgttct
     2161 cgtggattgt aggagtagag gactgctcgg tgattccgcg cacactttct tcctgcccag
     2221 tgaattcaca ctctagagtt gcgtctcaag ggcgatccgt tcagtctcgt ccaaggcggt
     2281 ggagattacc gagcagcatg
//
