LOCUS       sequence-369860-        3840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC30A4_STOP
FEATURES             Location/Qualifiers
     source          1..3840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1397..1496)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1513..1532)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1514..1532)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1537..1553)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1537..1553)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1655..1674)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1666..2475
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1733..1752)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2622..3210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3111..3130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3364..3381
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3540..3567)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3659..3745)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3794..3816
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3808..3825
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3809..3825
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc aggaagcgga
      121 gcatggaaga ggctgaagtc catgctgcgc aaggacgatg cccctctgtt tctgaacgat
      181 accagcgcct tcgacttcag cgacgaggca ggcgacgagg gactgtcccg gttcaacaag
      241 ctgagagtgg tggtggcaga cgatggatct gaggcaccag agaggcctgt gaatggagca
      301 caccccaccc tgcaggcaga cgatgactcc ctgctggatc aggacctgcc tctgacaaac
      361 tcccagctgt ctctgaaggt ggattcctgc gacaattgtt ctaagcagag agagatcctg
      421 aagcagagga aggtgaaggc aaggctgacc atcgcagccg tgctgtacct gctgtttatg
      481 atcggcgagc tggtgggcgg ctatatcgcc aatagcctgg ccatcatgac cgatgccctg
      541 cacatgctga cagacctgtc cgccatcatc ctgacactgc tggccctgtg gctgagctcc
      601 aagtctccaa ccaagaggtt cacatttggc ttccacaggc tggaggtgct gagcgccatg
      661 atctccgtgc tgctggtgta catcctgatg ggctttctgc tgtatgaggc cgtgcagagg
      721 accatccaca tgaactacga gatcaatggc gatatcatgc tgatcacagc agcagtggga
      781 gtggcagtga acgtgatcat gggcttcctg ctgaatcaga gcggacacag gcactctcac
      841 agccactccc tgccatctaa cagcccaacc aggggatctg gatgcgagag aaatcacgga
      901 caggatagcc tggccgtgcg ggcagccttt gtgcacgcac tgggcgacct ggtgcagtcc
      961 gtgggcgtgc tgatcgcagc ctacatcatc cggttcaagc ctgagtataa gatcgccgac
     1021 ccaatctgta cctacgtgtt cagcctgctg gtggccttta ccacattcag gatcatctgg
     1081 gatacagtgg tcatcatcct ggagggcgtg ccaagccacc tgaacgtgga ctacatcaag
     1141 gaggccctga tgaagatcga ggacgtgtac tccgtggagg acctgaatat ctggagcctg
     1201 acctccggca agtctacagc catcgtgcac atccagctga tccccggctc tagctccaag
     1261 tgggaggagg tgcagtctaa ggccaaccac ctgctgctga ataccttcgg catgtacaga
     1321 tgcacaatcc agctgcagag ctatcggcag gaggtggata gaacatgcgc caactgtcag
     1381 tctagctccc cctaagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     1441 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1501 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1561 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1621 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1681 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1741 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1801 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1861 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1921 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     1981 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2041 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     2101 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     2161 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     2221 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     2281 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     2341 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2401 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2461 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2521 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     2581 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2641 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2701 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2761 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2821 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2881 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2941 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3001 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3061 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3121 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3181 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3241 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3301 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3361 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3421 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3481 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     3541 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     3601 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     3661 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     3721 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     3781 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
