LOCUS       sequence-370129-       12370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PROSS-designed high-redox potential laccase from Trametes 
            versicolor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12370)
  AUTHORS   Barber-Zucker S, Mateljak I, Goldsmith M, Kupervaser M, Alcalde M, 
            Fleishman SJ
  TITLE     Designed High-Redox Potential Laccases Exhibit High Functional 
            Diversity.
  JOURNAL   ACS Catal. 2022 Nov 4;12(21):13164-13173. doi: 
            10.1021/acscatal.2c03006. Epub 2022 Oct 13.
  PUBMED    36366766
REFERENCE   2  (bases 1 to 12370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscatal.2c03006"; journalName: "ACS Catal"; date: 
            "2022-11-4- 4"; volume: "12"; issue: "21"; pages: "13164-13173"
COMMENT     Sequence Label: pJRoC30_Tv9nL
FEATURES             Location/Qualifiers
     source          1..12370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    830..877
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      complement(1205..1660)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1342..1363)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1554..1573
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1682..2123
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    1682..1799
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2094..2117
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             join(2150..2206,2207..2404,2405..2416)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTADLFAASSALAAPVKTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKRETEA"
     CDS             3929..3946
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             3953..3970
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4007..4254
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4023..4041)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4331..4348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4502..5090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4582..4601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5261..6052,6053..6121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKKLTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5884..5903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(6217..7020)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7021..7236)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     protein_bind    complement(10136..10183)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(10336..11607)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
ORIGIN
        1 actatacaaa tctattgcta taataatagg aagattgccc gagaaagaca agaagagagc
       61 taccgaaatg ctcatgagaa aaatggattg tacacagtta ttagtcccac cagctccaac
      121 ggaagaagat gttatgaagc tcgtaagcgt cgttacccaa ttgcttactt tagttccacc
      181 agatcgtcaa gctgctttaa taggtgattt attcatcccg gaatctctaa aggatatatt
      241 caatagtttc aatgaactgg cggcagagaa tcgtttacag caaaaaaaga gtgagttgga
      301 aggaaggact gaagtgaacc atgctaatac aaatgaagaa gttccctcca ggcgaacaag
      361 aagtagagac acaaatgcaa gaggagcata taaattacaa aacaccatca ctgagggccc
      421 taaagcggtt cccacgaaaa aaaggagagt agcaacgagg gtaaggggca gaaaatcacg
      481 taatacttct agggtatgat ccaatatcaa aggaaatgat agcattgaag gatgagacta
      541 atccaattga ggagtggcag catatagaac agctaaaggg tagtgctgaa ggaagcatac
      601 gataccccgc atggaatggg ataatatcac aggaggtact agactacctt tcatcctaca
      661 taaatagacg catataagta cgcatttaag cataaacacg cactatgccg ttcttctcat
      721 gtatatatat atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt
      781 gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat
      841 tccgaagttc ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag
      901 cgctctgaag acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa
      961 atattgcgaa taccgcttcc acaaacattg ctcaaaagta tctctttgct atatatctct
     1021 gtgctatatc cctatataac ctacccatcc acctttcgct ccttgaactt gcatctaaac
     1081 tcgacctcta cattttttat gtttatctct agtattactc tttagacaaa aaaattgtag
     1141 taagaactat tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt
     1201 agagaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     1261 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     1321 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     1381 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     1441 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     1501 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     1561 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     1621 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggatcgat ccactagtcc
     1681 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
     1741 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
     1801 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
     1861 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
     1921 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
     1981 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
     2041 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
     2101 ctctatactt taacgtcaag gagaaaaaac tataggatcc ataggatcca tgagatttcc
     2161 ttcaattttt actgctgatt tattcgcagc atcctccgca ttagctgctc cagtcaaaac
     2221 tacaacagaa gatgaaacgg cacaaattcc ggctgaagct gtcatcggtt actcagattt
     2281 agaaggggat ttcgatgttg ctgttttgcc attttccaac agcacaaata acggattatt
     2341 gtttataaat actactattg ccagcattgc tgctaaagaa gaaggggtat ctctcgagaa
     2401 aagagagact gaagctgagt tcgcgatcgg tccagttgcg actctacaca ttacaaatgc
     2461 acaggtggct cctgacgggt ttacgaggga cgctatagtc gtgaacggtc aattccctgg
     2521 cccactaata acaggcaata agggcgaccg tttccagtta aacgtcatca atcagttgac
     2581 aaatccaact atgttaaaga gcacatcaat ccactggcat gggttcttcc agcacgggac
     2641 taattgggca gatggccctg ctttcgtcac acagtgccct attgccccgg gtcactcctt
     2701 tctttacgat ttcacggttc cagaccaggc ggggacattc tggtatcaca gtcacctatc
     2761 aacacagtac tgcgatggat taaggggtcc atttgttgtg tacgatccga acgatcctca
     2821 cgctcactta tatgacgtcg acgacgaaag caccgttatc acattggccg actggtatca
     2881 cgtagctgca aggctgggcc ccaggttccc gttaggggct gacgcaacgt taataaacgg
     2941 tttaggtcgt tcatcatcga cgcccacagc ccctttagcg gtgattaatg ttcagcccgg
     3001 aaagaggtac agatttagac taatatccat cagttgcgac gcaaattata ctttctcaat
     3061 cgacggacac aatatgacca taatagaggt agacggaatc ccaactcaac cgtacaccgt
     3121 agactcgatt cagatattcg ctgcccaacg ttactccttc atccttaatg caaatcagcc
     3181 agtgggaaac tactggatac gtgccaatcc taattttggt actactggtt tcgatggtgg
     3241 cataaactca gcaatcctaa ggtacgaggg cgcaccggat gcggaaccta ccacaacaca
     3301 gacaacttcg actataccgc taaacgagac caacttacac cctcttgagc ctatgccagt
     3361 cccaggggag cccacgccag gtggtgtcga ttacgcattg aatttagatt tcaacttcaa
     3421 cggcacaaat ttcttcatca ataatgcgac attcgtacca ccctctgttc cagtgttact
     3481 tcaaatatta agtggtgctc aaacggccca ggaccttcta cctagtggta gcgtctatcc
     3541 attaccccct aataaggtca tagaaataac tttccccgcg acgtccaacg ctccgggtgc
     3601 tccgcaccca ttccacctac atgggcacca attcgcagtt gtccgttccg ccggaagtac
     3661 cgagtacaat tatgacaatc ctatatggag agacgtggtc tctaccggaa ctccagctaa
     3721 tggggacaac gttactatca ggtttagaac tgataacccc gggccttggt ttcttcactg
     3781 ccacatagat tggcatcttg aggccggatt tgccatcgtt ttcgctgagg acattgataa
     3841 tgttaagtct gcaaaccctg taccacaggc atggaaggac ctgtgcgaca tatacaacgc
     3901 tctaccccct gcaaaccaga gcagcggttt agttccccgc ggcagcagcg cgcaccacca
     3961 ccaccaccac taagcggccg ctcgagcatg catctagagg gccgcatcat gtaattagtt
     4021 atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt
     4081 agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt attgagaacg
     4141 ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt
     4201 atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcaagctg
     4261 cggccctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4321 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4381 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4441 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4501 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4561 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4621 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4681 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4741 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4801 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4861 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4921 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4981 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5041 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5101 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5161 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5221 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5281 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5341 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5401 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5461 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5521 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5581 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5641 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagcttc ttcggtcctc
     5701 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     5761 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     5821 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     5881 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     5941 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6001 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6061 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6121 tactcttcct ttttcaatgg gtaataactg atataattaa attgaagctc taatttgtga
     6181 gtttagtata catgcattta cttataatac agttttttag ttttgctggc cgcatcttct
     6241 caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc
     6301 ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc
     6361 cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt
     6421 cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc
     6481 gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt
     6541 agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt
     6601 tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc
     6661 attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac
     6721 tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga
     6781 taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg
     6841 tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt
     6901 ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag
     6961 cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat
     7021 gatttatctt cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga atactgggca
     7081 atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt
     7141 ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa
     7201 tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagc tagcaggtac gtagtatata
     7261 gagacaaaat agaagaaacc gttcataatt ttctgaccaa tgaagaatca tcaacgctat
     7321 cactttctgt tcacaaagta tgcgcaatcc acatcggtat agaatataat cggggatgcc
     7381 tttatcttga aaaaatgcac ccgcagcttc gctagtaatc agtaaacgcg ggaagtggag
     7441 tcaggctttt tttatggaag agaaaataga caccaaagta gccttctttt aaccttaacg
     7501 gacctacagt gcaaaaagtt atcaagagac tgcattatag agcgcacaaa ggagaaaaaa
     7561 agtaatctaa gatgctttgt tagaaaaata gcgctctcgg gatgcatttt tgtagaacaa
     7621 aaaagaagta tagattcttt gttggtaaaa tagcgctctc gcgttgcatt tctgttctgt
     7681 aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttt
     7741 tacaaaaatg aagcacagat tcttcgttgg taaaatagcg ctttcgcgtt gcatttctgt
     7801 tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt
     7861 tgttctacaa aatgaagcac agatgcttcg ttaacaaaga tatgctattg aagtgcaaga
     7921 tggaaacgca gaaaatgaac cggggatgcg acgtgcaaga ttacctatgc aatagatgca
     7981 atagtttctc caggaaccga aatacataca ttgtcttccg taaagcgcta gactatatat
     8041 tattatacag gttcaaatat actatctgtt tcagggaaaa ctcccaggtt cggatgttca
     8101 aaattcaatg atgggtaaca agtacgatcg taaatctgta aaacagtttg tcggatatta
     8161 ggctgtatct cctcaaagcg tattcgaata tcattgagaa gctgcagcgt cacatcggat
     8221 aataatgatg gcagccattg tagaagtgcc ttttgcattt ctagtctctt tctcggtcta
     8281 gctagtttta ctacatcgcg aagatagaat cttagatcac actgcctttg ctgagctgga
     8341 tcaatagagt aacaaaagag tggtaaggcc tcgttaaagg acaaggacct gagcggaagt
     8401 gtatcgtaca gtagacggag tatactagta tagtctatag tccgtggaat tctaagtgcc
     8461 agctttataa tgtcattctc cttactacag acccgcctga aagtagacac atcatcatca
     8521 gtaagctttg acaaaaagca ttgagtagct aactcttcta tgcaatctat agctgtttta
     8581 taaggcattc aatggacaga ttgaggtttt tgaaacatac tagtgaaatt agccttaatc
     8641 ccttctcgaa gttaatcatg cattatggtg taaaaaatgc aactcgcgtt gctctacttt
     8701 ttcccgaatt tccaaatacg cagctggggt gattgctcga tttcgtaacg aaagttttgt
     8761 ttataaaaac cgcgaaaacc ttctgtaaca gatagatttt tacagcgctg atatacaatg
     8821 acatcagctg taatggaaaa taactgaaat atgaatggcg agagactgct tgcttgtatt
     8881 aagcaatgta ttatgcagca cttccaacct atggtgtacg atgaaagtag gtgtgtaatc
     8941 gagacgacaa gggggacttt tccagttcct gacaattata agaaatacaa aacgttagca
     9001 tttgcatttg ttggacatgt actgaataca gacgacacac cggtaattga aaaagaactg
     9061 gattggcctg atcctgcact agtgtacaat acaattgtcg atcgaatcat aaatcaccca
     9121 gaattatcac agtttatatc ggttgcattt attagtcagt taaaggccac catcggagag
     9181 ggtttagata ttaatgtaaa aggcacgcta aaccgcaggg gaaagggtat cagaaggcct
     9241 aaaggcgtat tttttagata catggaatct ccatttgtca atacaaaggt cactgcattc
     9301 ttctcttatc ttcgagatta taataaaatt gcctcagaat atcacaataa tactaaattc
     9361 attctcacgt tttcatgtca agcatattgg gcatctggcc caaacttctc cgccttgaag
     9421 aatgttatta ggtgctccat aattcatgaa tacatttcta agtttgtgga aagagaacag
     9481 gataaaggtc atataggaga tcaggagcta ccgcctgaag aggacccttc tcgtgaacta
     9541 aacaatgtac aacatgaagt caatagttta acggaacaag atgcggaggc ggatgaagga
     9601 ttgtggggtg aaatagattc attatgtgaa aaatggcagt ctgaagcgga agatcaaact
     9661 gaggcggaga taatagccga caggataatt ggaaatagcc agaggatggc gaacctcaaa
     9721 attcgtcgta caaagttcaa aagtgtcttg tatcatatac taaaggaact aattcaatct
     9781 cagggaaccg taaaggttta tcgcggtagt agtttttcac acgattcgat aaagataagc
     9841 ttacattatg aagagcagca tattacagcc gtatgggtct acttgacagt aaaatttgaa
     9901 gagcattgga agcctgttga tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag
     9961 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg
    10021 agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt
    10081 ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt
    10141 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac
    10201 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc
    10261 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta
    10321 tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc
    10381 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag
    10441 ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc
    10501 ctttgatatt ggatcatatg catagtaccg agaaactagt gcgaagtagt gatcaggtat
    10561 tgctgttatc tgatgagtat acgttgtcct ggccacggca gaagcacgct tatcgctcca
    10621 atttcccaca acattagtca actccgttag gcccttcatt gaaagaaatg aggtcatcaa
    10681 atgtcttcca atgtgagatt ttgggccatt ttttatagca aagattgaat aaggcgcatt
    10741 tttcttcaaa gctttattgt acgatctgac taagttatct tttaataatt ggtattcctg
    10801 tttattgctt gaagaattgc cggtcctatt tactcgtttt aggactggtt cagaattcct
    10861 caaaaattca tccaaatata caagtggatc gatcctaccc cttgcgctaa agaagtatat
    10921 gtgcctacta acgcttgtct ttgtctctgt cactaaacac tggattatta ctcccagata
    10981 cttattttgg actaatttaa atgatttcgg atcaacgttc ttaatatcgc tgaatcttcc
    11041 acaattgatg aaagtagcta ggaagaggaa ttggtataaa gtttttgttt ttgtaaatct
    11101 cgaagtatac tcaaacgaat ttagtatttt ctcagtgatc tcccagatgc tttcaccctc
    11161 acttagaagt gctttaagca tttttttact gtggctattt cccttatctg cttcttccga
    11221 tgattcgaac tgtaattgca aactacttac aatatcagtg atatcagatt gatgtttttg
    11281 tccatagtaa ggaataattg taaattccca agcaggaatc aatttcttta atgaggcttc
    11341 cagaattgtt gctttttgcg tcttgtattt aaactggagt gatttattga caatatcgaa
    11401 actcagcgaa ttgcttatga tagtattata gctcatgaat gtggctctct tgattgctgt
    11461 tccgttatgt gtaatcatcc aacataaata ggttagttca gcagcacata atgctatttt
    11521 ctcacctgaa ggtctttcaa acctttccac aaactgacga acaagcacct taggtggtgt
    11581 tttacataat atatcaaatt gtggcatgct tagcgccgat cttgtgtgca attgatatct
    11641 agtttcaact actctattta tcttgtatct tgcagtattc aaacacgcta actcgaaaaa
    11701 ctaactttaa ttgtcctgtt tgtctcgcgt tctttcgaaa aatgcaccgg ccgcgcatta
    11761 tttgtactgc gaaaataatt ggtactgcgg tatcttcatt tcatatttta aaaatgcacc
    11821 tttgctgctt ttccttaatt tttagacggc ccgcaggttc gttttgcggt actatcttgt
    11881 gataaaaagt tgttttgaca tgtgatctgc acagatttta taatgtaata agcaagaata
    11941 cattatcaaa cgaacaatac tggtaaaaga aaaccaaaat ggacgacatt gaaacagcca
    12001 agaatctgac ggtaaaagca cgtacagctt atagcgtctg ggatgtatgt cggctgttta
    12061 ttgaaatgat tgctcctgat gtagatattg atatagagag taaacgtaag tctgatgagc
    12121 tactctttcc aggatatgtc ataaggccca tggaatctct cacaaccggt aggccgtatg
    12181 gtcttgattc tagcgcagaa gattccagcg tatcttctga ctccagtgct gaggtaattt
    12241 tgcctgctgc gaagatggtt aaggaaaggt ttgattcgat tggaaatggt atgctctctt
    12301 cacaagaagc aagtcaggct gccatagatt tgatgctaca gaataacaag ctgttagaca
    12361 atagaaagca
//
