LOCUS       sequence-370151-       11759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PROSS-designed versatile peroxidase 11H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Barber-Zucker S, Mindel V, Garcia-Ruiz E, Weinstein JJ, Alcalde M, 
            Fleishman SJ
  TITLE     Stable and Functionally Diverse Versatile Peroxidases Designed 
            Directly from Sequences.
  JOURNAL   J Am Chem Soc. 2022 Mar 2;144(8):3564-3571. doi: 
            10.1021/jacs.1c12433. Epub 2022 Feb 18.
  PUBMED    35179866
REFERENCE   2  (bases 1 to 11759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.1c12433"; journalName: "J Am Chem Soc"; date: 
            "2022-03-2- 2"; volume: "144"; issue: "8"; pages: "3564-3571"
COMMENT     Sequence Label: pJRoC30_VP11H
FEATURES             Location/Qualifiers
     source          1..11759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..177
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             join(209..265,266..463,464..475)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
     terminator      1532..1779
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1548..1566)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(1856..1873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2027..2615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2107..2126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2786..3577,3578..3646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3409..3428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3742..4545)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4546..4761)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     protein_bind    complement(7661..7708)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    10649..10696
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      complement(11024..11479)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11161..11182)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11373..11392
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(11501..11759,1..183)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    11501..11618
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 ctggggtaat taatcagcga agcgatgatt tttgatctat taacagatat ataaatgcaa
       61 aaactgcata accactttaa ctaatacttt caacattttc ggtttgtatt acttcttatt
      121 caaatgtaat aaaagtatca acaaaaaatt gttaatatac ctctatactt taacgtcaag
      181 gagaaaaaac tataggatca taggatccat gagatttcct tcaattttta ctgctgtttt
      241 attcgcagca tcctccgcat tagctgctcc agtcaacact acaacagaag atgaaacggc
      301 acaaattccg gctgaagctg tcatcggtta ctcagattta gaaggggatt tcgatgttgc
      361 tgttttgcca ttttccaaca gcacaaataa cgggttattg tttataaata ctactattgc
      421 cagcattgct gctaaagaag aaggggtatc tctcgagaaa agagaggctg aagctgaatt
      481 cgtaaccctt cctcaaaaga gggcaacatg ttcaggcggc cagaccactt cgaatgctgc
      541 gtgttgcgtc ctatttgact tacgtgacga cttgcagaag aatttattcg acggcggaca
      601 gtgcggcgag gaagtacatg agtctcttcg tttaaccttc cacgatgcca taggcttcag
      661 tccgactaag ggtggcggtg gggcagacgg atcagttctt atcttttcag ataccgagtt
      721 aaacttccct gcaaacttag gaatcgacga aattgtagaa gctcagaaac ccttcttaca
      781 aaggcacaat atctctgctg gtgacttagt ccaatttgct ggcgcccttg gagtttctaa
      841 ctgtccaggc gccccacgta tcccattctt cctgggtaga ccgcctgcta ctgcgccatc
      901 ccctgacgga ttagtgcctg agcctttcga ctcagttgat gacatcctag cacgtatggc
      961 tgacgccggc tttagtcctg tcgaggttgt ttggttgctg tcatctcaca ccatcgccgc
     1021 agctgatcac gtggacccta ctattccggg gacccctttt gactcaacac cgtcaatctt
     1081 cgattcacag ttcttcatag agacacagtt gagaggtact ctgttccctg gtactggtgg
     1141 caaccctggc gaggttgaga gtccactacc gggcgagatc agactacagt ctgatcacct
     1201 actagctagg gaccctagaa ccgcctgcga atggcagtcc atggttgata acatgccgaa
     1261 gattcagaac cgttttgctg caacgatgct taaaatgagc ttattgggac agaacgtcag
     1321 ggatttgatc gactgctccg atgtgatacc aactccacca cccctggtcg gcactgcaca
     1381 cttgccagcc ggtaaaactc aatctgacgt agagcaggcc tgcgcaacaa ctccgtttcc
     1441 cacgattcct gccgatcctg ggcctgtaac atcggtccca ccggtaccac ctagttgagc
     1501 ggccgctcga gcatgcatct agagggccgc atcatgtaat tagttatgtc acgcttacat
     1561 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
     1621 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
     1681 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
     1741 cttgagaagg ttttgggacg ctcgaaggct ttaatttgca agctgcggcc ctgcattaat
     1801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2461 ctagaagrac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3661 aatgggtaat aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc
     3721 atttacttat aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca
     3781 gcctgctttt ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc
     3841 tcttccaaca ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg
     3901 ttgacccaat gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc
     3961 gtaaccttca tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt
     4021 gtcgctcttc gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt
     4081 gcatgacaat tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc
     4141 ctgcttcaaa ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc
     4201 ccattctgct attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc
     4261 agcaaatttt ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg
     4321 cttaactgtg ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca
     4381 aattttggga cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa
     4441 tgaagcacac aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg
     4501 actaggatga gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt
     4561 cctgcaggtt tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc
     4621 aacactacat atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt
     4681 ctgttcggag attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata
     4741 aaaaaaaaat gatgaattga aaagctagca ggtacgtagt atatagagac aaaatagaag
     4801 aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt tctgttcaca
     4861 aagtatgcgc aatccacatc ggtatagaat ataatcgggg atgcctttat cttgaaaaaa
     4921 tgcacccgca gcttcgctag taatcagtaa acgcgggaag tggagtcagg ctttttttat
     4981 ggaagagaaa atagacacca aagtagcctt cttctaacct taacggacct acagtgcaaa
     5041 aagttatcaa gagactgcat tatagagcgc acaaaggaga aaaaaagtaa tctaagatgc
     5101 tttgttagaa aaatagcgct ctcgggatgc atttttgtag aacaaaaaag aagtatagat
     5161 tctttgttgg taaaatagcg ctctcgcgtt gcatttctgt tctgtaaaaa tgcagctcag
     5221 attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttttacaa aaatgaagca
     5281 cagattcttc gttggtaaaa tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag
     5341 ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttc tacaaaatga
     5401 agcacagatg cttcgttaac aaagatatgc tattgaagtg caagatggaa acgcagaaaa
     5461 tgaaccgggg atgcgacgtg caagattacc tatgcaatag atgcaatagt ttctccagga
     5521 accgaaatac atacattgtc ttccgtaaag cgctagacta tatattatta tacaggttca
     5581 aatatactat ctgtttcagg gaaaactccc aggttcggat gttcaaaatt caatgatggg
     5641 taacaagtac gatcgtaaat ctgtaaaaca gtttgtcgga tattaggctg tatctcctca
     5701 aagcgtattc gaatatcatt gagaagctgc agcgtcacat cggataataa tgatggcagc
     5761 cattgtagaa gtgccttttg catttctagt ctctttctcg atctagctag ttttactaca
     5821 tcgcgaagat agaatcttag atcacactgc ctttgctgag ctggatcaat agagtaacaa
     5881 aagagtggta aggcctcgtt aaaggacaag gacctgagcg gaagtgtatc gtacagtaga
     5941 cggagtatac tagtatagtc tatagtccgt ggaattctaa gtgccagctt tataacgtca
     6001 ttctccttac tacagacccg cctgaaagta gacacatcat catcagtaag ctttgacaaa
     6061 aagcattgag tagctaactc ttctatgcaa tctatagctg ttttataagg cattcaatgg
     6121 acagattgag gtttttgaaa catactagtg aaattagcct taatcccttc tcgaagttaa
     6181 tcatgcatta tggtgtaaaa aatgcaactc gcgttgctct actttttccc gaatttccaa
     6241 atacgcagct ggggtgattg ctcgatttcg taacgaaagt tttgtttata aaaaccgcga
     6301 aaaccttctg taacagatag atttttacag cgctgatata caatgacatc agctgtaatg
     6361 gaaaataact gaaatatgaa tggcgagaga ctgcttgctt gtattaagca atgtattatg
     6421 cagcacttcc aacctatggt gtacgatgaa agtaggtgtg taatcgagac gacaaggggg
     6481 acttttccag ttcctgacaa ttataagaaa tacaaaacgt tagcatttgc atttgttgga
     6541 catgtactga atacagacga cacaccggta attgaaaaag aactggattg gcctgatcct
     6601 gcactagtgt acaatacaat tgtcgatcga atcataaatc acccagaatt atcacagttt
     6661 atatcggttg catttattag tcagttaaag gccaccatcg gagagggttt agatattaat
     6721 gtaaaaggca cgctaaaccg caggggaaag ggtatcagaa ggcctaaagg cgtatttttt
     6781 agatacatgg aatctccatt tgtcaataca aaggtcactg cattcttctc ttatcttcga
     6841 gattataata aaattgcctc agaatatcac aataatacta aattcattct cacgttttca
     6901 tgtcaagcat attgggcatc tggcccaaac ttctccgcct tgaagaatgt tattaggtgc
     6961 tccataattc atgaatacat ttctaagttt gtggaaagag aacaggataa aggtcatata
     7021 ggagatcagg agctaccgcc tgaagaggac ccttctcgtg aactaaacaa tgtacaacat
     7081 gaagtcaata gtttaacgga acaagatgcg gaggcggatg aaggattgtg gggtgaaata
     7141 gattcattat gtgaaaaatg gcagtctgaa gcggaagatc aaactgaggc ggagataata
     7201 gccgacagga taattggaaa tagccagagg atggcgaacc tcaaaattcg tcgtacaaag
     7261 ttcaaaagtg tcttgtatca tatactaaag gaactaattc aatctcaggg aaccgtaaag
     7321 gtttatcgcg gtagtagttt ttcacacgat tcgataaaga taagcttaca ttatgaagag
     7381 cagcatatta cagccgtatg ggtctacttg acagtaaaat ttgaagagca ttggaagcct
     7441 gttgatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
     7501 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
     7561 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
     7621 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
     7681 agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa
     7741 tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg
     7801 cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac
     7861 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc
     7921 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg
     7981 ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc
     8041 atatgcatag taccgagaaa ctagtgcgaa gtagtgatca ggtattgctg ttatctgatg
     8101 agtatacgtt gtcctggcca cggcagaagc acgcttatcg ctccaatttc ccacaacatt
     8161 agtcaactcc gttaggccct tcattgaaag aaatgaggtc atcaaatgtc ttccaatgtg
     8221 agattttggg ccatttttta tagcaaagat tgaataaggc gcatttttct tcaaagcttt
     8281 attgtacgat ctgactaagt tatcttttaa taattggtat tcctgtttat tgcttgaaga
     8341 attgccggtc ctatttacwc gwwktakrws tgsytmmkaa ykcytswmww wktcwyyswm
     8401 aywwammast ggatyrwtmc tmccmswtrc kywtwwkgra ytaatttrrm tkattwcggm
     8461 tcaacgttct taatatcgct gaatcttcca caattgatga aagtagctag gaagaggaat
     8521 tggtataaag tttttgtttt tgtaaatctc gaagtatact caaacgaatt tagtattttc
     8581 tcagtgatct cccagatgct ttcaccctca cttagaagtg ctttaagcat ttttttactg
     8641 tggctatttc ccttatctgc ttcttccgat gattcgaact gtaattgcaa actacttaca
     8701 atatcagtga tatcagattg atgtttttgt ccatagtaag gaataattgt aaattcccaa
     8761 gcaggaatca atttctttaa tgaggcttcc agaattgttg ctttttgcgt cttgtattta
     8821 aactggagtg atttattgac aatatcgaaa ctcagcgaat tgcttatgat agtattatag
     8881 ctcatgaatg tggctctctt gattgctgtt ccgttatgtg taatcatcca acataaatag
     8941 gttagttcag cagcacataa tgctattttc tcacctgaag gtctttcaaa cctttccaca
     9001 aactgacgaa caagcacctt aggtggtgtt ttacataata tatcaaattg tggcatgctt
     9061 agcgccgatc ttgtgtgcaa ttgatatcta gtttcaacta ctctatttat cttgtatctt
     9121 gcagtattca aacacgctaa ctcgaaaaac taactttaat tgtcctgttt gtctcgcgtt
     9181 ctttcgaaaa atgcaccggc cgcgcattat ttgtactgcg aaaataattg gtactgcggt
     9241 atcttcattt catattttaa aaatgcacct ttgctgcttt tccttaattt ttagacggcc
     9301 cgcaggttcg ttttgcggta ctatcttgtg ataaaaagtt gttttgacat gtgatctgca
     9361 cagattttat aatgtaataa gcaagaatac attatcaaac gaacaatact ggtaaaagaa
     9421 aaccaaaatg gacgacattg aaacagccaa gaatctgacg gtaaaagcac gtacagctta
     9481 tagcgtctgg gatgtatgtc ggctgtttat tgaaatgatt gctcctgatg tagatattga
     9541 tatagagagt aaacgtaagt ctgatgagct actctttcca ggatatgtca taaggcccat
     9601 ggaatctctc acaaccggta ggccgtatgg tcttgattct agcgcagaag attccagcgt
     9661 atcttctgac tccagtgctg aggtaatttt gcctgctgcg aagatggtta aggaaaggtt
     9721 tgattcgatt ggaaatggta tgctctcttc acaagaagca agtcaggctg ccatagattt
     9781 gatgctacag aataacaagc tgttagacaa tagaaagcaa ctatacaaat ctattgctat
     9841 aataatagga agattgcccg agaaagacaa gaagagagct accgaaatgc tcatgagaaa
     9901 aatggattgt acacagttat tagtcccacc agctccaacg gaagaagatg ttatgaagct
     9961 cgtaagcgtc gttacccaat tgcttacttt agttccacca gatcgtcaag ctgctttaat
    10021 aggtgattta ttcatcccgg aatctctaaa ggatatattc aatagtttca atgaactggc
    10081 ggcagagaat cgtttacagc aaaaaaagag tgagttggaa ggaaggactg aagtgaacca
    10141 tgctaataca aatgaagaag ttccctccag gcgaacaaga agtagagaca caaatgcaag
    10201 aggagcatat aaattacaaa acaccatcac tgagggccct aaagcggttc ccacgaaaaa
    10261 aaggagagta gcaacgaggg taaggggcag aaaatcacgt aatacttcta gggtatgatc
    10321 caatatcaaa ggaaatgata gcattgaagg atgagactaa tccaattgag gagtggcagc
    10381 atatagaaca gctaaagggt agtgctgaag gaagcatacg ataccccgca tggaatggga
    10441 taatatcaca ggaggtacta gactaccttt catcctacat aaatagacgc atataagtac
    10501 gcatttaagc ataaacacgc actatgccgt tcttctcatg tatatatata tacaggcaac
    10561 acgcagatat aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt
    10621 cggaagcgct cgttttcgga aacgctttga agttcctatt ccgaagttcc tattctctag
    10681 aaagtatagg aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca
    10741 aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca
    10801 caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc
    10861 tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg
    10921 tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga
    10981 atcgaaaaca atacgaaaat gtaaacattt cctatacgta gagaaattgt aagcgttaat
    11041 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
    11101 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
    11161 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
    11221 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
    11281 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
    11341 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
    11401 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
    11461 gcgccgctac agggcgcgtg gggatcgatc cactagtcca cggattagaa gccgccgagc
    11521 gggtgacagc cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt
    11581 tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac
    11641 tagcttttat ggttatgaag aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa
    11701 tgaacgaatc aaattaacaa ccataggatg ataatgcgat tagtttttta gccttattt
//
