LOCUS       sequence-370243-       10447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-On inducible expression of HaloTag-iPAK4 in a lentiviral 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Lin D, Li X, Moult E, Park P, Tang B, Shen H, Grimm JB, Falco N, Jia
            BZ, Baker D, Lavis LD, Cohen AE
  TITLE     Time-tagged ticker tapes for intracellular recordings.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7.
  PUBMED    36593408
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7."
COMMENT     Sequence Label: DL147_plenti_TRE_HT-iPAK4
FEATURES             Location/Qualifiers
     source          1..10447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(259..1050,1051..1119))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     882..901
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1120..1224)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1299..1318)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1490..1869
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1871..2069
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2087..2267
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2314..2439
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2932..3165
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3350..3394
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3692..3809
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     3979..4001
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3993..4010
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3994..4010
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4020..4039
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4020..4038
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4063..4377
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4071..4089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4107..4125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4142..4160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4178..4196
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4214..4232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4249..4267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4285..4303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4350..4374
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    6391..6979
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6444..6464)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6862..6873)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6982..6998)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6983..6999)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7504..7684
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7710..7727)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7716..7940
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7986..8414
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8073..8092)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8283..8304
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8423..8443)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8428..8757
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8608..8743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8670..8689
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8805..8852
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8871..9245
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9375..9496
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9412..9431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9466..9485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9545..9561)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9545..9561)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9558..9580)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9569..9585
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9593..9599,9600..9617,9618..9623))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9638..9659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9776..9793)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9947..10447,1..88))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10027..10046)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
       61 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
      121 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
      181 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
      241 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
      301 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
      361 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
      421 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
      481 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
      541 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
      601 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
      661 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
      721 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
      781 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
      841 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
      901 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
      961 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     1021 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     1081 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     1141 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     1201 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     1261 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     1321 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     1381 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     1441 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     1501 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     1561 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1621 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1681 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1741 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1801 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1861 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     1921 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     1981 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     2041 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     2101 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2161 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2221 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2281 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     2341 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     2401 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     2461 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     2521 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     2581 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     2641 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     2701 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     2761 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     2821 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     2881 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     2941 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     3001 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     3061 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     3121 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     3181 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     3241 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     3301 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     3361 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     3421 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3481 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3541 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3601 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
     3661 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     3721 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     3781 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     3841 tttggttaat taaccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     3901 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     3961 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     4021 aatacgactc actatagggc gaattggctt tcgtcttcac tcgagtttac tccctatcag
     4081 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta
     4141 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
     4201 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg
     4261 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg
     4321 cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     4381 aaccggtgcc accatgatcg gtactggctt tccattcgac ccccattatg tggaagtcct
     4441 gggcgagcgc atgcactacg tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct
     4501 gcacggtaac ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac
     4561 ccatcgctgc attgctccag acctgatcgg tatgggcaaa tccgacaaac cagacctggg
     4621 ttatttcttc gacgaccacg tccgcttcat ggatgccttc atcgaagccc tgggtctgga
     4681 agaggtcgtc ctggtcattc acgactgggg ctccgctctg ggtttccact gggccaagcg
     4741 caatccagag cgcgtcaaag gtattgcatt tatggagttc atccgcccta tcccgacctg
     4801 ggacgaatgg ccagaatttg cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg
     4861 ccgcaagctg atcatcgatc agaacgtttt tatcgagggt acgctgccga tgggtgtcgt
     4921 ccgcccgctg actgaagtcg agatggacca ttaccgcgag ccgttcctga atcctgttga
     4981 ccgcgagcca ctgtggcgct tcccaaacga gctgccaatc gccggtgagc cagcgaacat
     5041 cgtcgcgctg gtcgaagaat acatggactg gctgcaccag tcccctgtcc cgaagctgct
     5101 gttctggggc accccaggcg ttctgatccc accggccgaa gccgctcgcc tggccaaaag
     5161 cctgcctaac tgcaaggctg tggacatcgg cccgggtctg aatctgctgc aagaagacaa
     5221 cccggacctg atcggcagcg agatcgcgcg ctggctgtcg acgctcgaga tttccggcga
     5281 gccaaccact tccggatccg aagcagagga ctggacagca gccctactga acaggggtcg
     5341 cagtcgccag cccctggtac taggggacaa ttgctttgct gacttggtgc acaactggat
     5401 ggagctgcct gaggaattcc ccgccgcccc tgctgttcct gggccccctg gcccccgctc
     5461 accacagcgg gagccacagc gagtatccca tgagcagttc cgggctgccc tgcagctggt
     5521 ggtggaccca ggcgaccccc gctcctacct ggacaacttc atcaagattg gcgagggctc
     5581 cacgggcatc gtgtgcatcg ccaccgtgcg cagctcgggc aagctggtgg ccgtcaagaa
     5641 gatggacctg cgcaagcagc agaggcgcga gctgctcttc aacgaggtgg taatcatgag
     5701 ggactaccag cacgagaatg tggtggagat gtacaacagc tacctggtgg gggacgagct
     5761 ctgggtggtc atggagttcc tggaaggagg cgccctcacc gacatcgtca cccacaccag
     5821 gatgaacgag gagcagatcg cagccgtgtg ccttgcagtg ctgcaggccc tgtcggtgct
     5881 ccacgcccag ggcgtcatcc accgggacat caagagcgac tcgatcctgc tgacccatga
     5941 tggcagggtg aagctgtcag actttgggtt ctgcgcccag gtgagcaagg aagtgccccg
     6001 aaggaagtcg ctggtcggca cgccctactg gatggcccca gagctcatct cccgccttcc
     6061 ctacgggcca gaggtagaca tctggtcgct ggggataatg gtgattgaga tggtggacgg
     6121 agagcccccc tacttcaacg agccacccct caaagccatg aagatgattc gggacaacct
     6181 gccaccccga ctgaagaacc tgcacaaggt gtcgccatcc ctgaagggct tcctggaccg
     6241 cctgctggtg cgagaccctg cccagcgggc cacggcagcc gagctgctga agcacccatt
     6301 cctggccaag gcagggccgc ctgccagcat cgtgcccctc atgcgccaga accgcaccag
     6361 atgagcggcc gcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     6421 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6481 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6541 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6601 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6661 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6721 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6781 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6841 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6901 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6961 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
     7021 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     7081 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     7141 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7201 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     7261 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     7321 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     7381 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     7441 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     7501 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7561 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7621 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7681 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     7741 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     7801 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     7861 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7921 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     7981 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8041 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8101 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8161 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     8221 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8281 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8341 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8401 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     8461 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     8521 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8581 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8641 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     8701 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     8761 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     8821 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     8881 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     8941 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     9001 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     9061 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     9121 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     9181 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     9241 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     9301 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     9361 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9421 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9481 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     9541 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9601 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9661 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9721 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9781 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9841 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9901 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9961 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    10021 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10081 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10141 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10201 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10261 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10321 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10381 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10441 ttggtag
//
