LOCUS       sequence-370300-       14094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBolux harboring the promoter region upstream of BT3332.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Modesto JL, Pearce VH, Townsend GE 2nd
  TITLE     Harnessing gut microbes for glycan detection and quantification.
  JOURNAL   Nat Commun. 2023 Jan 17;14(1):275. doi: 10.1038/s41467-022-35626-2.
  PUBMED    36650134
REFERENCE   2  (bases 1 to 14094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-01-17"; volume: "14(1):275. doi"; pages: " 
            10.1038/s41467-022-35626-2"
COMMENT     Sequence Label: P-BT3332
FEATURES             Location/Qualifiers
     source          1..14094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     463..481
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(519..541)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     641..660
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3562..3584
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3576..3593
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3577..3593
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3976..5418
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             7607..8686
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLL"
     primer_bind     complement(9720..9736)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9720..9736)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9733..9755)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9744..9760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9768..9774,9775..9792,9793..9798))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9813..9834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9951..9968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10122..10710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10202..10221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10881..11672,11673..11741))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11504..11523
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11742..11846)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atctaacaaa agtaggagga ttttttaact ttttaccata gacattttat tagggaaacg
       61 taggttcaac tggtaagtac aaataagtag aaatgtttga acaacactgt tttaggaagt
      121 tgaaaaatca agtttctctc tcggtatagc gtttattttg gccaatatct tctttagttt
      181 ggcatttgag tatttcccat tcgtgttcta tttagctcct tattatgagt atgtagcaag
      241 ttgcttatca aattcagcct ctttgagggt caaatatggt tttgcaaatg gtgactgaca
      301 agacataaac aaggtcagca ggatttttta cataaatgga catgaatgta tataacgggg
      361 tttgagggcc aatggaacga aaacgtacgt taaggagata attcgttgtt tatatttaaa
      421 tttagctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
      481 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
      541 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
      601 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
      661 ctgagagtgc accattcccc gaccgatgat ttttaagtga ctgattttgt gctgttttgg
      721 gggtatatta agaataaaag aaatagaata agttaagtac ttgatacaca atatagggca
      781 ttttccatat tggaaattct cattttccaa tctagaaaat accgatttcc taatatgata
      841 ctaaatagga aaataatatt tcccttaata ttgtttttat ggaaaataat agtttacttt
      901 gtggagaata atatttccca aaaacatatc aaaatggaaa ataaaaaagc agttaagtta
      961 accgattttc aaaagaacga agaaaatcct tttatgaaac aagctataga aggtattgaa
     1021 aatcatgttg ttaaaaagta taagagtaat agtggtggcg ataagagagc tgtagtagct
     1081 ttagccgaca ctgaaactgg agaagtgttt aagacttcgt ttatccgtca aatagaagta
     1141 gatgaagaac aattcactaa attgtatctt tctaactttg ctgcattctt tgacctatca
     1201 caagcagcta ttcgggtttt tggttacttt atgacctgca tgaaacccaa aaatgattta
     1261 atcattttca atagaaaaaa atgcctagaa tataccaaat acaaaacaga caaagccgtt
     1321 tataaaggac ttgcagaact tgtaaaagct gaaatcatag cccgaggacc agccgataat
     1381 ctttggttta ttaatcctct gatagtattc aatggtgacc gagtgacatt tgctaaaaca
     1441 tacgttcgga aaaagacttt agctgcccaa aagaaagaag aagcagagaa acgacaatta
     1501 tcacttggct ttgatgaaca gtaacactcc attgagtgaa gctgccgttt ggtcgctccc
     1561 cctttggggg cgggggggga tagataaagt tcctctatgt aaagttataa tgggggatga
     1621 aaggcaaggt tcgctaacct taccccgatt gtttatgctt cccagcattc aatctatccc
     1681 cgtttatggg tttctctttc accctacaaa gataacctca tgggggaaaa atgtccaaga
     1741 atatggggta aaaactatca agtcggtaga aaataagtat ctttgaagta catttttagt
     1801 agaggtactg gtgtgcctag gcgaagcaga taggcaaaaa taaagccgct ggagatgtcg
     1861 gatgtccgtc aattaactcc agcggcttgt attttggtaa ctctcccgta tcggtcagct
     1921 accgtttggt cagcaacaaa ggtagtactt tattatcgca aatccaaatg tttttccctt
     1981 tatttgtgca tttacaaaac attcactaac ttcgcaccta tgcagtatgg tgatagcaca
     2041 ctagcaccct aaagagtgcg tgtgtaaggg gacaagcccc cttaaccccc tgtcagccgg
     2101 ctgtcaccgg ctgttatcga ttgtcacaaa aatgttaact tatgggagca acaagtattc
     2161 atgtacaagc agtgaagccg gggagcgaga ttcacaactt tagggaaaaa gagttggact
     2221 atgttcgtcc cgaacttagt catttgaatg aaagctgggt tggagatagc atttcccatc
     2281 ggctggagag tgcaaaacaa agatatctcg atacggttgg gcagaagatg caggctaaag
     2341 ccgcacccat acgagaggga gtaatagtaa tcaaacaaga aaccactatg caggaactcc
     2401 agcagtttgc cacggtctgc aaagaacgtt tcggtatcga agcgtttcaa atccatatac
     2461 acaaggacga aggatacatg aacgcaaagc agtggacacc taaccttcat gcccatgtag
     2521 ttttcgattg gacgcagccg aatgggaaaa gtgtgcgttt atcgcgtgat gacatggcag
     2581 aactccaaac catagcatct gaaacactgg gcatggaacg tggcgtttct tctgaccgca
     2641 aacacttatc ggctatgcag tacaagaccg aatgtgcgaa agaacagctt caggaactat
     2701 ccaacgatat atccagtgca ttagacaaat acaaggacgc acaaaaccag cttcttcagc
     2761 ttcagaagga actacgttcc attgaaacaa agaagaacgt ccaaaaactc atttctaagg
     2821 cttcagagaa gttttacggc ttgattggaa aaacagtcaa cgatagggaa aaggatgcct
     2881 taaaagccaa agtaaagact ttagaggggg aaaatgaaca actatccgat agactgggaa
     2941 aggctatact tgaaaaagag cgaaacagca caaaggcatt caaagccgag aatgacaagg
     3001 agtattatag acaacaaatg gacaacgcaa ggacaaccag taacctatta aggacggaga
     3061 accaaaaact aaaagccgaa accaaggagt tgaaaaaaga acttggtaag atgaaagact
     3121 tgttcaattc cgaacaactg gaggctttaa ggcatcattt cccaaacata tcaaaagcta
     3181 tggaagaggg gaaagaccta ttcaagcaaa tcaccagaag cagaggtttc ggcatgggta
     3241 tgtaaccccc ccccgccccc aaagggggag cgaccaaacg gcagcctctc tcaatggagt
     3301 gttacaccgt tcggattcag aaaacatttt gcagggttcg gaaaacggtt gtgtgcttat
     3361 tctaaaaaaa gtttatggtg tgtatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     3421 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     3481 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     3541 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg
     3601 agctcggtac ccggggatcc aaaatggaac tgggcaatga caggcatccc cagctgtacg
     3661 atttatccgc cgatccatcg gagaaaaaca atgtagcgaa acagcatccg gaagttgtca
     3721 gggagctgtc cgaactgctt gaatcggtaa agacaagata accaatgaga atagcagatt
     3781 tgtattattt tgtatcaatc ttgaataatt ctatccatat gagaacaatc aatgcgtata
     3841 tctttgccat cgtaaattca acaaaatcac ttaattttta atatatgaaa aaacatctat
     3901 ccaaccagac aagaaaaagg actagtcact cccgcatttt aaaataaaat aaattattta
     3961 tttaattaaa cgaatatgac taaaaaaatt tcattcatta ttaacggcca ggttgaaatc
     4021 tttcccgaag gtgatgattt agtgcaatcc attaattttg gtgataatag tgtttacctg
     4081 ccaatattga atgactctca tgtaaaaaac attattgatt gtaatggaaa taacgaatta
     4141 cggttgcata acattgtcaa ttttctctat acggtagggc aaagatggaa aaatgaagaa
     4201 tactcaagac gcaggacata cattcgtgac ttaaaaaaat atatgggata ttcagaagaa
     4261 atggctaagc tagaggccaa ttggatatct atgattttat gttctaaagg cggcctttat
     4321 gatgttgtag aaaatgaact tggttctcgc catatcatgg atgaatggct acctcaggat
     4381 gaaagttatg ttcgggcttt tccgaaaggt aaatctgtac atctgttggc aggtaatgtt
     4441 ccattatctg ggatcatgtc tatattacgc gcaattttaa ctaagaatca gtgtattata
     4501 aaaacatcgt caaccgatcc ttttaccgct aatgcattag cgttaagttt tattgatgta
     4561 gaccctaatc atccgataac gcgctcttta tctgttatat attggcccca ccaaggtgat
     4621 acatcactcg caaaagaaat tatgcaacat gcggatgtta ttgtcgcttg gggagggcca
     4681 gatgcgatta attgggcggt agagcatgcg ccatcttatg ctgatgtgat taaatttggt
     4741 tctaaaaaga gtctttgcat tatcgataat cctgttgatt tgacgtccgc agcgacaggt
     4801 gcggctcatg atgtttgttt ttacgatcag cgagcttgtt tttctgccca aaacatatat
     4861 tacatgggaa atcattatga ggaatttaag ttagcgttga tagaaaaact taatctatat
     4921 gcgcatatat taccgaatgc caaaaaagat tttgatgaaa aggcggccta ttctttagtt
     4981 caaaaagaaa gcttgtttgc tggattaaaa gtagaggtgg atattcatca acgttggatg
     5041 attattgagt caaatgcagg tgtggaattt aatcaaccac ttggcagatg tgtgtacctt
     5101 catcacgtcg ataatattga gcaaatattg ccttatgttc aaaaaaataa gacgcaaacc
     5161 atatctattt ttccttggga gtcatcattt aaatatcgag atgcgttagc attaaaaggt
     5221 gcggaaagga ttgtagaagc aggaatgaat aacatatttc gagttggtgg atctcatgac
     5281 ggaatgagac cgttgcaacg attagtgaca tatatttctc atgaaaggcc atctaactat
     5341 acggctaagg atgttgcggt tgaaatagaa cagactcgat tcctggaaga agataagttc
     5401 cttgtatttg tcccgtaatc cgcatggcaa attgtaaatt gtaaatcgta aaatagtaat
     5461 atattaatgg aaaataaatc caaatataaa accatcgacc acgttatttg tgttgaagga
     5521 aataaaaaaa ttcatgtttg ggaaacgctg ccagaagaaa acagcccaaa gagaaagaat
     5581 gccattatta ttgcgtctgg ttttgcccgc aggatggatc attttgctgg tctggcggaa
     5641 tatttatcgc ggaatggatt tcatgtgatc cgctatgatt cgcttcacca cgttggattg
     5701 agttcaggga caattgatga atttacaatg tctataggaa agcagagctt gttagcagtg
     5761 gttgattggt taactacacg aaaaataaat aacttcggta tgttggcttc aagcttatct
     5821 gcgcggatag cttatgcaag cctatctgaa atcaatgctt cgtttttaat caccgcagtc
     5881 ggtgttgtta acttaagata ttctcttgaa agagctttag ggtttgatta tctcagtcta
     5941 cccattaatg aattgccgaa taatctagat tttgaaggcc ataaattggg tgctgaagtc
     6001 tttgcgagag attgtcttga ttttggttgg gaagatttag cttctacaat taataacatg
     6061 atgtatcttg atataccgtt tattgctttt actgcaaata acgataattg ggtcaagcaa
     6121 gatgaagtta tcacattgtt atcaaatatt cgtagtaatc gatgcaagat atattctttg
     6181 ttaggaagtt cgcatgactt gagtgaaaat ttagtggtcc tgcgcaattt ttatcaatcg
     6241 gttacgaaag ccgctatcgc gatggataat gatcatctgg atattgatgt tgatattact
     6301 gaaccgtcat ttgaacattt aactattgcg acagtcaatg aacgccgaat gagaattgag
     6361 attgaaaatc aagcgatttc gctgtcttaa taaggaggag gataaagaat atgacttcat
     6421 atgttgataa acaagaaatt acagcaagct cagaaattga tgatttgatt ttttcgagcg
     6481 atccattagt gtggtcttac gacgagcagg aaaaaatcag aaagaaactt gtgcttgatg
     6541 catttcgtaa tcattataaa cattgtcgag aatatcgtca ctactgtcag gcacacaaag
     6601 tagatgacaa tattacggaa attgatgaca tacctgtatt cccaacatcg gtttttaagt
     6661 ttactcgctt attaacttct caggaaaacg agattgaaag ttggtttacc agtagcggca
     6721 cgaatggttt aaaaagtcag gtggcgcgtg acagattaag tattgagaga ctcttaggct
     6781 ctgtgagtta tggcatgaaa tatgttggta gttggtttga tcatcaaata gaattagtca
     6841 atttgggacc agatagattt aatgctcata atatttggtt taaatatgtt atgagtttgg
     6901 tggaattgtt atatcctacg acatttaccg taacagaaga acgaatagat tttgttaaaa
     6961 cattgaatag tcttgaacga ataaaaaatc aagggaaaga tctttgtctt attggttcgc
     7021 catactttat ttatttactc tgccattata tgaaagataa aaaaatctca ttttctggag
     7081 ataaaagcct ttatatcata accggaggcg gctggaaaag ttacgaaaaa gaatctctga
     7141 aacgtgatga tttcaatcat cttttatttg atactttcaa tctcagtgat attagtcaga
     7201 tccgagatat atttaatcaa gttgaactca acacttgttt ctttgaggat gaaatgcagc
     7261 gtaaacatgt tccgccgtgg gtatatgcgc gagcgcttga tcctgaaacg ttgaaacctg
     7321 tacctgatgg aacgccgggg ttgatgagtt atatggatgc gtcagcaacc agttatccag
     7381 catttattgt taccgatgat gtcgggataa ttagcagaga atatggtaag tatcccggcg
     7441 tgctcgttga aattttacgt cgcgtcaata cgaggacgca gaaagggtgt gctttaagct
     7501 taaaccaagc atttaatagt tgatataaat gaagggtgaa gaaggatgaa tgaaggtaac
     7561 actcataaac tcgaaattct tcattcttaa tttttaatta aaatatatga aatttggaaa
     7621 ctttttgctt acataccaac ctccccaatt ttctcaaaca gaggtaatga aacgtttggt
     7681 taaattaggt cgcatctctg aggagtgtgg ttttgatacc gtatggttac tggagcatca
     7741 tttcacggag tttggtttgc ttggtaaccc ttatgtcgct gctgcatatt tacttggcgc
     7801 gactaaaaaa ttgaatgtag gaactgccgc tattgttctt cccacagccc atccagtacg
     7861 ccaacttgaa gatgtgaatt tattggatca aatgtcaaaa ggacgatttc ggtttggtat
     7921 ttgccgaggg ctttacaaca aggactttcg cgtattcggc acagatatga ataacagtcg
     7981 cgccttagcg gaatgctggt acgggctgat aaagaatggc atgacagagg gatatatgga
     8041 agctgataat gaacatatca agttccataa ggtaaaagta aaccccgcgg cgtatagcag
     8101 aggtggcgca ccggtttatg tggtggctga atcagcttcg acgactgagt gggctgctca
     8161 atttggccta ccgatgatat taagttggat tataaatact aacgaaaaga aagcacaact
     8221 tgagctttat aatgaagtgg ctcaagaata tgggcacgat attcataata tcgaccattg
     8281 cttatcatat ataacatctg tagatcatga ctcaattaaa gcgaaagaga tttgccggaa
     8341 atttctgggg cattggtatg attcttatgt gaatgctacg actatttttg atgattcaga
     8401 ccaaacaaga ggttatgatt tcaataaagg gcagtggcgt gactttgtat taaaaggaca
     8461 taaagatact aatcgccgta ttgattacag ttacgaaatc aatcccgtgg gaacgccgca
     8521 ggaatgtatt gacataattc aaaaagacat tgatgctaca ggaatatcaa atatttgttg
     8581 tggatttgaa gctaatggaa cagtagacga aattattgct tccatgaagc tcttccagtc
     8641 tgatgtcatg ccatttctta aagaaaaaca acgttcgcta ttataaagaa aggaataaga
     8701 caatggatat gaaatttgga ttgttcttcc ttaacttcat caattcaaca actgttcaag
     8761 aacaaagtat agttcgcatg caggaaataa cggagtatgt tgataagttg aattttgaac
     8821 agattttagt gtatgaaaat catttttcag ataatggtgt tgtcggcgct cctctgactg
     8881 tttctggttt tctgctcggt ttaacagaga aaattaaaat tggttcatta aatcacatca
     8941 ttacaactca tcatcctgtc cgcatagcgg aggaagcttg cttattggat cagttaagtg
     9001 aagggagatt tattttaggg tttagtgatt gcgaaaaaaa agatgaaatg cattttttta
     9061 atcgcccggt tgaatatcaa cagcaactat ttgaagagtg ttatgaaatc attaacgatg
     9121 ctttaacaac aggctattgt aatccagata acgattttta tagcttccct aaaatatctg
     9181 taaatcccca tgcttatacg ccaggcggac ctcggaaata tgtaacagca accagtcatc
     9241 atattgttga gtgggcggcc aaaaaaggta ttcctctcat ctttaagtgg gatgattcta
     9301 atgatgttag atatgaatat gctgaaagat ataaagccgt tgcggataaa tatgacgttg
     9361 acctatcaga gatagaccat cagttaatga tattagttaa ctataacgaa gatagtaata
     9421 aagctaaaca agagacgcgt gcatttatta gtgattatgt tcttgaaatg caccctaatg
     9481 aaaatttcga aaataaactt gaagaaataa ttgcagaaaa cgctgtcgga aattatacgg
     9541 agtgtataac tgcggctaag ttggcaattg aaaagtgtgg tgcgaaaagt gtattgctgt
     9601 cctttgaacc aatgaatgat ttgatgagcc aaaaaaatgt aatcaatatt gttgatgata
     9661 atattaaaaa gtaccacatg taagtcgacc tgcaggcatg caagcttggc gtaatcatgg
     9721 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9781 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9841 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9901 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    10021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    10081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10561 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10801 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10861 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10921 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10981 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    11041 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    11101 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    11161 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11221 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11281 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11341 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11401 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11461 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11521 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11581 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11641 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11701 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11761 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11821 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcgtctatt
    11881 tttttattgc caagttctaa tgcttctatc tcctgccatt catagaggct attatatgcc
    11941 atacatccga taagaatgga gataagtata tatcccaact gtattgcctt atagaaattt
    12001 cctgaccgct ccattatttt gatgacattg atttttggaa catgaataaa agtttatctt
    12061 tttcgttcat gcggatatta tcagaataac cgccttttgt tatttgatac ccgcatggct
    12121 taaccataaa aatgcctaag cccttagtgt atgaacttac ttctgatgca tagtctttac
    12181 ttgtatttaa tggaactttc cctttaatat gacaccactc attattgcaa ctgatgtctt
    12241 caatctcaga catcattttt tgcaaatctg taatagcttt ggaacttgcc gcttggggta
    12301 tctgcaactc aaaatagagc atcggttcga gaatgtccac acctgactgt tgcaaggcca
    12361 gcctgaagac ataaggggtc agctgtctga aatcagcagg tgtacttacc gggctataat
    12421 actcggcttg agtaaaagtt actttcagat cagtcacttc ccatccatgt aacccggatt
    12481 ggcaagacat acgaatccct tcaaaaacgg cattttgaaa agaatggttc agataaccat
    12541 aggagatgtc actttcgatt tgcaaccctg tccctaacgg taagggttca agagtcagcc
    12601 ctattgtggc ccaataaggg ttgggcggca cttcgatctg aataatctta ttgacctttt
    12661 ttacaggtcg ttctttgtat atagtcttga tctcatcaaa atggaccttt acggaaaatc
    12721 gttcttccag caatgtctgt atgatttcct tttgggttaa accatataac gagatttcca
    12781 attcatcact atatgagttt atggaaaagg acaaagacgg gtcttcaatc cacaatgtat
    12841 tcagagcgga tatcaccttg cttctctctt cgggcctgtc tggccggacg gaggatttga
    12901 gagcgggatg ctgatgcgat aatccttgaa tcaaacaagg ttcagcacct aaataatttc
    12961 cgattcgaaa atcatccata tcctctacaa tcgcgatatc attggcgccc acttcatcaa
    13021 catttatctc tctgccctga ttgatagttt ttagattttt aatcttgatg aatttttccg
    13081 aatcgttgat tcttacaacg tctcgaagtc tcagacttcc gtcaattatt tttagaaaac
    13141 ttcttttatg tcctttgggg tcatgctcta tcttataaag ataagatgaa agtctgtttg
    13201 agaccgatgc cggaggaagt ataaaagaag tgatggcgtc caacaactca ttgataccga
    13261 tattgaacat tgctgatcca tgtagcaccg gatagacttt ggcttttgcc acaagagcga
    13321 ttatcgtatt ccaataatca gccggtgaaa tttcgctatc cgccaaatat cgttctaata
    13381 tattgtcgtc atggttgcat acaaattctt tgtattcttc ctttatatat gtttgggagc
    13441 aaaccggata aaccgatcca tcgacaacat tttgcataaa caggacatct tgagacagat
    13501 ttgcttttat atccagatac aaacgctcca aattcacacc ggctcggtca atcttattga
    13561 taaatataat tgtcgggatt tgcagcttct gtaaagtatt gaacagcaac tttgtctgcg
    13621 cttgtatgcc ttcctttgcg gataagatga ggactgctcc atcaagcatt ttgaatgtcc
    13681 gctccacttc cgcaataaaa tccatgtgtc ccggagtgtc aatgatattg catttcacac
    13741 cattccagat aatagatgtc gtagaagccc gaacagtaat tcctctacgt ttctctatat
    13801 ccatagagtc cgttatggtg tcaccattat ccacacagcc gcacttttcc gttgctccac
    13861 tggcaaacag cagattctcg gttacggaag tttttcctgc atcaatgtga gcaagaattc
    13921 ctaaatttat aatattcatt tggattaagc aataatatac tacaatagat gcattgtcga
    13981 aacgcacctt ttaatacctc ctcgtagcat atgagaacta caggattcct aactcgtatt
    14041 aatatgtata ttattactgc cgcataacga ttacaaaatt acacaaaaaa atat
//
