LOCUS       sequence-370624-       10246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector with a ubiquitin promoter expressing GFP, human 
            AKT1 ORF with a phosphodeficient mutation at Serine 473, and Cre 
            recombinase, separated by T2A motifs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10246)
  TITLE     Retroviral vectors for expression of AKT kinases with GFP and Cre 
            recombinase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRubiG-T2A-AKT1(S473A)-T2A-Cre
FEATURES             Location/Qualifiers
     source          1..10246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     177..196
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(277..294)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(448..1036)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(528..547)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1207..1998,1999..2067))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1830..1849
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2068..2172)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2240..2258
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        2358..2737
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2738..2941
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2892..2912
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2942..3117
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    3180..3379
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3580..3996
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3926..3948
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3964..3980
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        4067..5278
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5115..5134
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5326..5335
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5332..5334,5335..5337,5338..6048)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5332..5334,5335..5337,5338..6048)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5638..5657)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5985..6006
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6049..6102
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6109..7545
                     /codon_start=1
                     /gene="AKT1"
                     /product="human serine/threonine protein kinase activated 
                     by growth factors"
                     /label=Akt1
                     /translation="SDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVD
                     QREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQT
                     VADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKV
                     ILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRL
                     CFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLML
                     DKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYE
                     MMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAK
                     EIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSME
                     CVDSERRPHFPQFAYSASGTA"
     CDS             7546..7599
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7606..8634
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKCFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    8658..9246
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8711..8731)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9129..9140)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9249..9265)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9250..9266)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9381..9806
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(9909..9931)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10067..10087)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(10072..10246,1..155)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
       61 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      121 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tactggctta actatgcggc
      181 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
      241 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      301 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      361 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      841 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
      901 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      961 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1021 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1081 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1141 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1201 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1261 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1321 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1381 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1441 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1501 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1561 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1621 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1681 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1741 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1801 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     1861 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1921 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1981 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2041 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2101 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2161 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     2221 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattagc
     2281 ttggccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc
     2341 aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     2401 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2461 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2521 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2581 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2641 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2701 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2761 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2821 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2881 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2941 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cttccgatag actgcgtcgc
     3001 ccgggtaccc gtattcccaa taaagcctct tgctgtttgc atccgaatcg tggtctcgct
     3061 gttccttggg agggtctcct ctgagtgatt gactacccac gacgggggtc tttcatttgg
     3121 gggctcgtcc gggatttgga gacccctgcc cagggaccac cgacccacca ccgggaggta
     3181 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgtt tgatgttatg
     3241 cgcctgcgtc tgtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact
     3301 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt
     3361 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg
     3421 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
     3481 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt
     3541 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc
     3601 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     3661 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     3721 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     3781 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
     3841 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     3901 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     3961 tccctttatc cagccctcac tccttctcta ggcgccggaa ttgaagatct cacacagcgg
     4021 ccgcacacac ttcgaacctt aattaacccg tgtcggctcc agatctggcc tccgcgccgg
     4081 gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa
     4141 gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca
     4201 taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt
     4261 gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt
     4321 ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac
     4381 gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt
     4441 gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg
     4501 tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc
     4561 tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg
     4621 ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa
     4681 ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa
     4741 agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt
     4801 cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt
     4861 tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt
     4921 tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct
     4981 ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga
     5041 cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta
     5101 tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt
     5161 ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca
     5221 gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga
     5281 agcttgggct gcaggtcgac tctagaggat ccccgggtac cggtcgccac catggtgagc
     5341 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     5401 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     5461 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     5521 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     5581 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     5641 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     5701 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     5761 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     5821 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     5881 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     5941 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     6001 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagga gggcagagga
     6061 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctagtacaag cgacgtggct
     6121 attgtgaagg agggttggct gcacaaacga ggggagtaca tcaagacctg gcggccacgc
     6181 tacttcctcc tcaagaatga tggcaccttc attggctaca aggagcggcc gcaggatgtg
     6241 gaccaacgtg aggctcccct caacaacttc tctgtggcgc agtgccagct gatgaagacg
     6301 gagcggcccc ggcccaacac cttcatcatc cgctgcctgc agtggaccac tgtcatcgaa
     6361 cgcaccttcc atgtggagac tcctgaggag cgggaggagt ggacaaccgc catccagact
     6421 gtggctgacg gcctcaagaa gcaggaggag gaggagatgg acttccggtc gggctcaccc
     6481 agtgacaact caggggctga agagatggag gtgtccctgg ccaagcccaa gcaccgcgtg
     6541 accatgaacg agtttgagta cctgaagctg ctgggcaagg gcactttcgg caaggtgatc
     6601 ctggtgaagg agaaggccac aggccgctac tacgccatga agatcctcaa gaaggaagtc
     6661 atcgtggcca aggacgaggt ggcccacaca ctcaccgaga accgcgtcct gcagaactcc
     6721 aggcacccct tcctcacagc cctgaagtac tctttccaga cccacgaccg cctctgcttt
     6781 gtcatggagt acgccaacgg gggcgagctg ttcttccacc tgtcccggga gcgtgtgttc
     6841 tccgaggacc gggcccgctt ctatggcgct gagattgtgt cagccctgga ctacctgcac
     6901 tcggagaaga acgtggtgta ccgggacctc aagctggaga acctcatgct ggacaaggac
     6961 gggcacatta agatcacaga cttcgggctg tgcaaggagg ggatcaagga cggtgccacc
     7021 atgaagacct tttgcggcac acctgagtac ctggcccccg aggtgctgga ggacaatgac
     7081 tacggccgtg cagtggactg gtgggggctg ggcgtggtca tgtacgagat gatgtgcggt
     7141 cgcctgccct tctacaacca ggaccatgag aagctttttg agctcatcct catggaggag
     7201 atccgcttcc cgcgcacgct tggtcccgag gccaagtcct tgctttcagg gctgctcaag
     7261 aaggacccca agcagaggct tggcgggggc tccgaggacg ccaaggagat catgcagcat
     7321 cgcttctttg ccggtatcgt gtggcagcac gtgtacgaga agaagctcag cccacccttc
     7381 aagccccagg tcacgtcgga gactgacacc aggtattttg atgaggagtt cacggcccag
     7441 atgatcacca tcacaccacc tgaccaagat gacagcatgg agtgtgtgga cagcgagcgc
     7501 aggccccact tcccccagtt cgcctactcg gccagcggca cggccgaggg cagaggaagt
     7561 cttctaacat gcggtgacgt ggaggagaat cccggcccta gtacatccaa tttactgacc
     7621 gtacaccaaa atttgcctgc attaccggtc gatgcaacga gtgatgaggt tcgcaagaac
     7681 ctgatggaca tgttcaggga tcgccaggcg ttttctgagc atacctggaa aatgcttctg
     7741 tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata accggaaatg ctttcccgca
     7801 gaacctgaag atgttcgcga ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa
     7861 actatccagc aacatttggg ccagctaaac atgcttcatc gtcggtccgg gctgccacga
     7921 ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga aaacgttgat
     7981 gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt
     8041 tcactcatgg aaaatagcga tcgctgccag gatatacgta atctggcatt tctggggatt
     8101 gcttataaca ccctgttacg tatagccgaa attgccagga tcagggttaa agatatctca
     8161 cgtactgacg gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc
     8221 gcaggtgtag agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc
     8281 gtctctggtg tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt
     8341 gttgccgcgc catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa
     8401 gcaactcatc gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg
     8461 tctggacaca gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata
     8521 ccggagatca tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa ctatatccgt
     8581 aacctggata gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga ttagaattcg
     8641 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     8701 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     8761 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     8821 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     8881 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     8941 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     9001 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     9061 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     9121 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     9181 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     9241 ccccgcatcg ataccgtcga cctcgagata tcagtggtcc aggctctagt tttgactcaa
     9301 caatatcacc agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt
     9361 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     9421 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     9481 aggtcaggaa cagatggaac agggtcgacc ctagagaacc atcagatgtt tccagggtgc
     9541 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     9601 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     9661 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     9721 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     9781 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc
     9841 agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac
     9901 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     9961 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
    10021 gccatgaccc agtcacgtag cgatagcgga gtgtagatcc ggctgtggaa tgtgtgtcag
    10081 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    10141 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10201 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactc
//
