LOCUS       sequence-37064-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pBS-F2-TGT
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(334..354)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(334..352)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(373..389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(373..389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(386..408)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    397..413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(421..427,428..445,446..451))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    466..487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(604..621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gactcactat agggcgaatt gggtacccgc ccatatgctt gccctgtcga gtcctgcgac
       61 cgccgcttct ctcggtccga ccacctgacc cagcatatcc ggattcacac aggccagaag
      121 cccttccagt gtcgcatctg catgaggaac ttcagcctcc gtcaccacct cgttggtcac
      181 atccgcacac acaccggcga gaagcctttt gcatgcgaca tctgtgggag gaagtttgcc
      241 cagtccagcg acctgcagcg tcacaccaaa atccatacag gtgaaaagta atctagagcg
      301 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
      361 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      421 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      481 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      541 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      601 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      661 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      721 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      781 taggctccgc ccc
//
