LOCUS       sequence-370651-        9693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged human CAD hCPS1 TL in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9693)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 9693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD hCPS1 TL
FEATURES             Location/Qualifiers
     source          1..9693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    3..555
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(147..164)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     374..393
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             567..962
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1011..1604
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1667..2024
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2083..2499
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2429..2451
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2467..2483
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    2509..2883
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2821..2837
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      2910..2919
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9587..9610
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
       61 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
      121 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
      181 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
      241 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
      301 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
      361 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
      421 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
      481 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
      541 aacacgatga taatatggcc acaaccatgg ttcctttgtc tcaagaagaa tccaccctca
      601 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
      661 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
      721 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
      781 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
      841 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
      901 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
      961 aagtcgacga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     1021 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     1081 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat
     1141 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     1201 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     1261 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
     1321 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     1381 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     1441 ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
     1501 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     1561 tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg
     1621 atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa
     1681 cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt
     1741 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa
     1801 cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga
     1861 cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta ataggaggga
     1921 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg
     1981 ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt
     2041 tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact
     2101 cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg
     2161 gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac
     2221 gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag
     2281 gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa
     2341 gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct
     2401 cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga
     2461 tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc atatgagatc
     2521 ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac aagagttact
     2581 aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca cgaagtctgg
     2641 agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc tcacccttac
     2701 cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga acctcgctgg
     2761 aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga cggcatcgca
     2821 gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc atcctctaga
     2881 ctgccggatc tagctagtta attaaggatc catggcggcc ctagtgttgg aggacgggtc
     2941 ggtcctgcgg ggccagccct ttggggccgc cgtgtcgact gccggggaag tggtgtttca
     3001 aaccggcatg gtcggctacc ccgaggccct cactgatccc tcctacaagg cacagatctt
     3061 agtgctcacc tatcctctga tcggcaacta tggcatcccc ccagatgaaa tggatgagtt
     3121 cggtctctgc aagtggtttg aatcctcggg catccacgta gcagcactgg tagtgggaga
     3181 gtgctgtcct actcccagcc actggagtgc cacccgcacc ctgcatgagt ggctgcagca
     3241 gcatggcatc cctggcttgc aaggagtaga cactcgggag ctgaccaaga agttgcggga
     3301 acaggggtct ctgctgggga agctggtcca gaatggaaca gaaccttcaa gtctgccatt
     3361 cttggacccc aatgcccgcc ccctggtacc agaggtctcc attaagactc cacgggtatt
     3421 caatacaggg ggtgcccctc ggatccttgc tttggactgt ggcctcaagt ataatcagat
     3481 ccgatgcctc tgccagcgtg gggctgaggt cactgtggta ccctgggacc atgcactaga
     3541 cagccaagag tatgagggtc tcttcttaag taatgggcct ggtgaccctg cctcctatcc
     3601 cagtgtcgta tccacactga gccgtgtttt atctgagcct aatccccgac ctgtctttgg
     3661 gatctgcctg ggacaccagc tattggcctt agccattggg gccaagactt acaagatgag
     3721 atatgggaac cgaggccata accagccctg cttgttggtg ggctctgggc gctgctttct
     3781 gacatcccag aaccatgggt ttgctgtgga gacagactca ctgccagcag actgggctcc
     3841 tctcttcacc aacgccaatg atggttccaa tgaaggcatt gtgcacaaca gcttgccttt
     3901 cttcagtgtc cagtttcacc cagagcacca agctggccct tcagatatgg aactgctttt
     3961 cgatatcttt ctggaaactg tgaaagaggc cacagctggg aaccctgggg gccagacagt
     4021 tagagagcgg ctgactgagc gcctctgtcc ccctgggatt cccactcccg gctctggact
     4081 tccaccacca cgaaaggttc tgatcctggg ctcagggggc ctctccattg gccaagctgg
     4141 agaatttgac tactcgggct ctcaggcaat taaggccctg aaggaggaaa acatccagac
     4201 gttgctgatc aaccccaata ttgccacagt gcagacctcc caggggctgg ccgacaaggt
     4261 ctattttctt cccataacac ctcattatgt aacccaggtg atacgtaatg aacgccccga
     4321 tggtgtgtta ctgacttttg ggggccagac tgctctgaac tgtggtgtgg agctgaccaa
     4381 ggccggggtg ctggctcggt atggggtccg ggtcctgggc acaccagtgg agaccattga
     4441 gctgaccgag gatcgacggg cctttgctgc cagaatggca gagatcggag agcatgtggc
     4501 cccgagcgag gcagcaaatt ctcttgaaca ggcccaggca gccgctgaac ggctggggta
     4561 ccctgtgcta gtgcgtgcag cctttgccct gggtggcctg ggctctggct ttgcctctaa
     4621 cagggaggag ctctctgctc tcgtggcccc agcttttgcc cataccagcc aagtgctagt
     4681 agacaagtct ctgaagggat ggaaggagat tgagtacgag gtggtgagag acgcctatgg
     4741 caactgtgtc acggtgtgta acatggagaa cttggaccca ctgggcatcc acactggtga
     4801 gtccatagtg gtggccccta gccagacact gaatgacagg gagtatcagc tcctgaggca
     4861 gacagctatc aaggtgaccc agcacctggg aattgttggg gagtgcaatg tgcagtatgc
     4921 cttgaaccct gagtctgagc agtattacat cattgaagtg aatgccaggc tctctcgcag
     4981 ctctgccctg gccagtaagg ccacaggtta tccactggct tatgtggcag ccaagctagc
     5041 attgggcatc cctttgcctg agctcaggaa ctctgtgaca gggggtacag cagcctttga
     5101 acccagcgtg gattattgtg tggtgaagat tcctcgatgg gaccttagca agttcctgcg
     5161 agtcagcaca aagattggga gctgcatgaa gagcgttggt gaagtcatgg gcattgggcg
     5221 ttcatttgag gaggccttcc agaaggccct gcgcatggtg gatgagaact gtgtgggctt
     5281 tgatcacaca gtgaaaccag tcagcgatat ggagttggag actccaacag ataagcggat
     5341 ttttgtggtg gcagctgctt tgtgggctgg ttattcagtg gaccgcctgt atgagctcac
     5401 acgcatcgac cgctggttcc tgcaccgaat gaagcgtatc atcgcacatg cccagctgct
     5461 agaacaacac cgtggacagc ctttgccgcc agacctgctg caacaggcca agtgtcttgg
     5521 cttctcagac aaacagattg cccttgcagt tctgagcaca gagctggctg ttcgcaagct
     5581 gcgtcaggaa ctggggatct gtccagcagt gaaacagatt gacacagttg cagctgagtg
     5641 gccagcccag acaaattacc tatacctaac gtattggggc accacccatg acctcacctt
     5701 tcgaacacct catgtcctag tccttggctc tggcgtctac cgtattggct ctagcgttga
     5761 atttgactgg tgtgctgtag gctgcatcca gcagctccga aagatgggat ataagaccat
     5821 catggtgaac tataacccag agacagtcag caccgactat gacatgtgtg atcgactcta
     5881 ctttgatgag atctcttttg aggtggtgat ggacatctat gagctcgaga accctgaagg
     5941 tgtgatccta tccatgggtg gacagctgcc caacaacatg gccatggcgt tgcatcggca
     6001 gcagtgccgg gtgctgggca cctcccctga agccattgac tcggctgaga accgtttcaa
     6061 gttttcccgg ctccttgaca ccattggtat cagccagcct cagtggaggg agctcagtga
     6121 cctcgagtct gctcgccaat tctgccagac cgtggggtac ccctgtgtgg tgcgcccctc
     6181 ctatgtgctg agcggtgctg ctatgaatgt ggcctacacg gatggagacc tggagcgctt
     6241 cctgagcagc gcagcagccg tctccaaaga gcatcccgtg gtcatctcca agttcatcca
     6301 ggaggctaag gagattgacg tggatgccgt ggcctctgat ggtgtggtgg cagccatcgc
     6361 catctctgag catgtggaga atgcaggtgt gcattcaggt gatgcgacgc tggtgacccc
     6421 cccacaagat atcactgcca aaaccctgga gcggatcaaa gccattgtgc atgctgtggg
     6481 ccaggagcta caggtcacag gacccttcaa tctgcagctc attgccaagg atgaccagct
     6541 gaaagttatt gaatgcaacg tacgtgtctc tcgctccttc cccttcgttt ccaagacact
     6601 gggtgtggac ctagtagcct tggccacgcg ggtcatcatg ggggaagaag tggaacctgt
     6661 ggggctaatg actggttctg gagttgtggg agtaaaggtg cccatgtttt cctggccccg
     6721 gttgagggat gctgacccca ttctgagatg tgagatggct tccactggag aggtggccgg
     6781 ctttggggag agccgctgtg aggcatacct caaggccatg ctaagcactg gctttaagat
     6841 ccccaagaag aatatcctgc tgaccattgg cagctataag aacaaaagcg agctgctccc
     6901 aactgtgcgg ctactggaga gcctgggcta cagcctctat gccagtctcg gcacagctga
     6961 cttctacact gagcatggcg tcaaggtaac agctgtggac tggcactttg aggaggctgt
     7021 ggatggtgag tgcccaccac agcggagcat cctggagcag ctagctgaga aaaactttga
     7081 gctggtgatt aacctgtcaa tgcgtggagc tgggggccgg cgtctctctt cctttgtcac
     7141 caagggctac cgcacccgac gcttggccgc tgacttctcc gtgcccctaa tcatcgatat
     7201 caagtgcacc aaactctttg tggaggccct aggccagatc gggccagccc ctcctttgaa
     7261 ggtgcatgtt gactgtatga cctcccaaaa gcttgtgcga ctgccgggat tgattgatgt
     7321 ccatgtgcac ctgcgggaac caggtgggac acataaggag gactttgctt caggcacagc
     7381 cgctgccctg gctgggggta tcaccatggt gtgtgccatg cctaataccc ggccccccat
     7441 cattgacgcc cctgctctgg ccctggccca gaagctggca gaggctggcg cccggtgcga
     7501 ctttgcgcta ttccttgggg cctcgtctga aaatgcagga accttgggca ccgtggccgg
     7561 gtctgcagcc gggctgaagc tttacctcaa tgagaccttc tctgagctgc ggctggacag
     7621 cgtggtccag tggatggagc atttcgagac atggccctcc cacctcccca ttgtggctca
     7681 cgcggagcag caaaccgtgg ctgctgtcct catggtggct cagctcactc agcgctcagt
     7741 gcacatatgt cacgtggcac ggaaggagga gatcctgcta attaaagctg caaaggcacg
     7801 gggcttgcca gtgacctgcg aggtggctcc ccaccacctg ttcctaagcc atgatgacct
     7861 ggagcgcctg gggcctggga agggggaggt ccggcctgag cttggctccc gccaggatgt
     7921 ggaagccctg tgggagaaca tggctgtcat cgactgcttt gcctcagacc atgctcccca
     7981 taccttggag gagaagtgtg ggtccaggcc cccacctggg ttcccagggt tagagaccat
     8041 gctgccacta ctcctgacgg ctgtaagcga gggccggctc agcctggacg acctgctgca
     8101 gcgattgcac cacaatcctc ggcgcatctt tcacctgcct ccgcaggagg acacctatgt
     8161 ggaggtggat ctggagcatg agtggacaat tcccagccac atgcccttct ccaaggccca
     8221 ctggacacct tttgaagggc agaaagtgaa gggcaccgtc cgccgtgtgg tcctgcgagg
     8281 ggaggttgcc tatatcgatg ggcaggttct ggtacccccg ggctatggac aggatgtacg
     8341 gaagtggcca cagggggctg ttcctcagct cccaccctca gcccctgcca ctagtgagat
     8401 gaccacgaca cctgaaagac cccgccgtgg catcccaggg cttcctgatg gccgcttcca
     8461 tctgccgccc cgaatccatc gagcctccga cccaggtttg ccagctgagg agccaaagga
     8521 gaagtcctct cggaaggtag ccgagccaga gctgatggga acccctgatg gcacctgcta
     8581 ccctccacca ccagtaccga gacaggcatc tccccagaac ctggggaccc ctggcttgct
     8641 gcacccccag acctcacccc tgctgcactc attagtgggc caacatatcc tgtccgtcca
     8701 gcagttcacc aaggatcaga tgtctcacct gttcaatgtg gcacacacac tgcgtatgat
     8761 ggtgcagaag gagcggagcc tcgacatcct gaaggggaag gtcatggcct ccatgttcta
     8821 tgaagtgagc acacggacca gcagctcctt tgcagcagcc atggcccggc tgggaggtgc
     8881 tgtgctcagc ttctcggaag ccacatcgtc cgtccagaag ggcgaatccc tggctgactc
     8941 cgtgcagacc atgagctgct atgccgacgt cgtcgtgctc cggcaccccc agcctggagc
     9001 agtggagctg gccgccaagc actgccggag gccagtgatc aatgctgggg atggggtcgg
     9061 agagcacccc acccaggccc tgctggacat cttcaccatc cgtgaggagc tgggaactgt
     9121 caatggcatg acgatcacga tggtgggtga cctgaagcac ggacgcacag tacattccct
     9181 ggcctgcctg ctcacccagt atcgtgtcag cctgcgctac gtggcacctc ccagcctgcg
     9241 catgccaccc actgtgcggg ccttcgtggc ctcccgcggc accaagcagg aggaattcga
     9301 gagcattgag gaggcgctgc ctgacactga tgtgctctac atgactcgaa tccagaagga
     9361 acgatttggc tctacccagg agtacgaagc ttgctttggt cagttcatcc tcactcccca
     9421 catcatgacc cgggccaaga agaagatggt ggtgatgcac ccgatgcccc gtgtcaacga
     9481 gataagcgtg gaagtggact cggatccccg cgcagcctac ttccgccagg ctgagaacgg
     9541 catgtacatc cgcatggctc tgttagccac cgtgctgggc cgtttcgact acaaagacga
     9601 tgacgacaag taggaattcc tgcaggcctc gagggccggc gcgccgcggc cgctacgtaa
     9661 attccgcccc ccccccccnc tccctnnnsc ccc
//
