LOCUS       sequence-370653-        7920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-repressible expression of the Tau repeat domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Saha I, Yuste-Checa P, Da Silva Padilha M, Guo Q, Korner R, 
            Holthusen H, Trinkaus VA, Dudanova I, Fernandez-Busnadiego R, 
            Baumeister W, Sanders DW, Gautam S, Diamond MI, Hartl FU, Hipp MS
  TITLE     The AAA+ chaperone VCP disaggregates Tau fibrils and generates 
            aggregate seeds in a cellular system.
  JOURNAL   Nat Commun. 2023 Feb 2;14(1):560. doi: 10.1038/s41467-023-36058-2.
  PUBMED    36732333
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-02-2"; volume: "14(1):560. doi"; pages: " 
            10.1038/s41467-023-36058-2"
COMMENT     Sequence Label: Tet-off TauRD (P301L/V337M)
FEATURES             Location/Qualifiers
     source          1..7920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        172..486
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    216..234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    251..269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    287..305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    323..341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    358..376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    394..412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     459..483
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             911..940
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        1017..1527
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1446..1464
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1537..1929
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1939..1992
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1993..2610,2611..2616,2617..2652,2653..2655,2656..2691,
                     2692..2694,2695..2730,2731..2739)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(2779..2799)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3197..3208)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3413..3646
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3718..3839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3755..3774)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3809..3828
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3879..4014
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4014..4085
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4086..4154,4155..4946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4304..4323)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5117..5705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5606..5625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5859..5876
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5993..6014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6029..6034,6035..6052,6053..6059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6067..6083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6072..6094
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6091..6107
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6091..6107
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6126..6146
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6128..6146
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6174..6400
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6401..6581
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6628..6753
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7246..7479
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7664..7708
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccact ttggccgcga atcgatatgt cgagtttact
      181 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
      241 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      301 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
      361 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
      421 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
      481 atcgcctgga gaattggcta gcgccaccat gcagacagcc cccgtgccca tgccagacct
      541 gaagaatgtc aagtccaaga tcggctccac tgagaacctg aagcaccagc cgggaggcgg
      601 gaaggtgcag ataattaata agaagctgga tcttagcaac gtccagtcca agtgtggctc
      661 aaaggataat atcaaacacg tcctgggagg cggcagtgtg caaatagtct acaaaccagt
      721 tgacctgagc aaggtgacct ccaagtgtgg ctcattaggc aacatccatc ataaaccagg
      781 aggtggccag atggaagtaa aatctgagaa gcttgacttc aaggacagag tccagtcgaa
      841 gattgggtcc ctggacaata tcacccacgt ccctggcgga ggaaataaaa agattgaagg
      901 aggatccgga gaacagaaac tgattagcga agaagatctg tagctgcagc ctatggccac
      961 tttggtaggg acagcttccc atgggaagtg cccaaaaagc ttaaatattg aggtccgggg
     1021 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     1081 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     1141 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     1201 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     1261 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     1321 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     1381 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     1441 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     1501 gaccgaatca ccgacctctc tccccagcaa ttcaccatgg ttcctttgtc tcaagaagaa
     1561 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     1621 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     1681 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     1741 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     1801 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     1861 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     1921 tgggagggcc ccggtgccga aggtagaggt tctctcctca cttgtggtga tgttgaagaa
     1981 aaccctggtc caatgtctag actggacaag agcaaagtca taaactctgc tctggaatta
     2041 ctcaatgaag tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt
     2101 gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctggca
     2161 atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa
     2221 gactttctgc ggaacaacgc caagtcattc cgctgtgctc tcctctcaca tcgcgacggg
     2281 gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag
     2341 ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc
     2401 gtgggccact ttacactggg ctgcgtattg gaggatcagg agcatcaagt agcaaaagag
     2461 gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg
     2521 ttcgaccatc agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc
     2581 ctggagaaac agctaaagtg cgaaagcggc gggccggccg acgcccttga cgattttgac
     2641 ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac
     2701 gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga tcgatccaag
     2761 atatcgtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2821 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2881 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2941 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3001 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3061 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3121 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3181 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3241 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3301 ggccgcctcc ccgcctgttt cgcctcggcg tccggactag aggtaccttt aagaccaatg
     3361 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     3421 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     3481 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3541 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3601 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     3661 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     3721 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3781 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3841 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3901 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3961 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctttcaa
     4021 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4081 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4141 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4201 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4261 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4321 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4381 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4441 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4501 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4561 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4621 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4681 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4741 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4801 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4861 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4921 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4981 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5041 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5101 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5161 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5221 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5281 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5341 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5401 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5461 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5521 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5581 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5641 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5701 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5761 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5821 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5881 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     5941 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     6001 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     6061 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     6121 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     6181 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     6241 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     6301 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     6361 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     6421 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6481 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6541 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     6601 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     6661 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     6721 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     6781 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     6841 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     6901 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     6961 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     7021 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     7081 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     7141 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     7201 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     7261 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     7321 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     7381 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     7441 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     7501 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     7561 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     7621 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     7681 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     7741 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     7801 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     7861 cctcccaacc ccgaggggac aattctcgac ctcgagacaa atggcagtat tcatccacaa
//
