LOCUS       sequence-370768-       10266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in 
            mammalian cells and lentiviral vector for constitutive expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Mumford TR, Rae D, Brackhahn E, Idris A, Gonzalez-Martinez D, Pal 
            AA, Chung MC, Guan J, Rhoades E, Bugaj LJ
  TITLE     Simple visualization of submicroscopic protein clusters with a 
            phase-separation-based fluorescent reporter.
  JOURNAL   Cell Syst. 2024 Feb 21;15(2):166-179.e7. doi: 
            10.1016/j.cels.2024.01.005. Epub 2024 Feb 8.
  PUBMED    38335954
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2024.01.005"; journalName: "Cell Syst"; date: 
            "2024-02-21- 21"; volume: "15"; issue: "2"; pages: "166-179.e7"
COMMENT     Sequence Label: pHR_PGK_LaG17-mCherry-HOTag3
FEATURES             Location/Qualifiers
     source          1..10266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     267..286
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             742..1446
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(884..902)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1152..1171
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1738..2326
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1791..1811)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2209..2220)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2329..2345)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2330..2346)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2436..2669
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2694..2712)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2695..2712)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3002..3021)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3235..3254)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3354..3376
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3414..3432)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3500..3604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3605..3673,3674..4465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3823..3842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4636..5224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5125..5144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5378..5395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5465..5485)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5470..5799
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5650..5785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6933..6998
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7128..7148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7573..7707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7610..7629)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7664..7683
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7875..8508
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8555..8680
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9177..9410
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9595..9639
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9924..10041
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10101..10266,1..333)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10136..10159)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
       61 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
      121 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
      181 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
      241 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
      301 gctgttctcc tcttcctcat ctccgggcct ttcatggctg atgtgcaact cgtagagtcc
      361 ggtggcggac tcgtccaggc gggaggctcc ctgcgcctga gttgcgctgc gtccggaaga
      421 actattagca tggccgccat gagttggttc agacaggcgc ctggaaagga gagggagttc
      481 gtggcaggca taagtcgctc cgcagggtca gccgtccatg ccgactccgt aaagggacgc
      541 tttaccatct ccagagataa cacaaaaaac acgctctatc tccaaatgaa ttctttgaag
      601 gccgaggaca cagccgtgta ctactgcgcc gttaggacca gcggattttt tggaagcatt
      661 cctaggacag ggacagcctt tgattactgg ggccagggaa cgcaggttac cgtttccggc
      721 ggttcaacaa ttggcggttc agtgagcaag ggcgaggagg ataacatggc catcatcaag
      781 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      841 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      901 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      961 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     1021 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     1081 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1141 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1201 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     1261 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     1321 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     1381 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     1441 tacaagggat ccggcagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt
     1501 ggcagtgctg gtggtagtgc tggtggtagt gctggtggct ctagaggaga aatcgcaaag
     1561 agtctgaaag aaattgcaaa atccttgaag gaaatagcgt ggtcactgaa agaaattgcc
     1621 aagtccctca aagggtaaaa acggccgcaa ctcccacctg caacatgcgt gactgactga
     1681 ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct tatcgataat
     1741 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     1801 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     1861 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     1921 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     1981 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2041 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2101 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     2161 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     2221 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     2281 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     2341 cctcgaggga attaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     2401 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     2461 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     2521 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     2581 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     2641 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     2701 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     2761 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     2821 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     2881 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     2941 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     3001 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     3061 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     3121 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     3181 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     3241 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     3301 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     3361 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     3421 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     3481 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3541 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3601 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3661 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3721 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3781 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3841 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3901 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3961 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4021 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4081 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4141 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4201 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4261 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4321 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4381 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4441 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4501 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4561 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4621 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4681 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4741 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4801 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4861 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4921 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4981 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5041 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5101 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5161 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5221 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5281 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5341 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5401 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5461 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     5521 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5581 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     5641 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5701 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5761 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     5821 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     5881 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     5941 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     6001 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     6061 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     6121 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     6181 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     6241 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     6301 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     6361 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     6421 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     6481 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     6541 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     6601 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     6661 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     6721 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     6781 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     6841 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     6901 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     6961 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     7021 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     7081 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     7141 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     7201 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     7261 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     7321 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     7381 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     7441 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     7501 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     7561 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7621 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7681 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     7741 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     7801 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     7861 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     7921 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     7981 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     8041 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     8101 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     8161 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     8221 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     8281 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     8341 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8401 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8461 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     8521 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     8581 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     8641 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     8701 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     8761 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     8821 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     8881 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     8941 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     9001 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     9061 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     9121 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     9181 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     9241 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     9301 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     9361 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     9421 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     9481 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     9541 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     9601 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     9661 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     9721 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     9781 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     9841 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
     9901 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     9961 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
    10021 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggatc
    10081 gataagcttg atatcgaatt gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
    10141 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
    10201 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
    10261 ttctac
//
