LOCUS       sequence-371114-        9241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  chromosomal integration downstream of glmS gene via Tn7 system of a 
            cassette encoding for arabinose-inducible GFP and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Avraham, Roi
  TITLE     Bacterial fluorescent reporters and tags toolkit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: R6K_BAD_mCherry-GFP_ChInt--
FEATURES             Location/Qualifiers
     source          1..9241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(218..931)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(258..278)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     873..901
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             940..948
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(975..1259)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             1286..2164
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(2344..2363)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     terminator      complement(2421..2448)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2540..2626)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2857..3567)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYMKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3135..3154)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3404..3422
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     RBS             3576..3584
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(3611..3895)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     protein_bind    complement(4191..4238)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(4315..4320,4321..4337,4338..4343)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4454..5248
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4508..4527)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5118..5137
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(5300..5347)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(5398..5484)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(5587..5681)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(5709..5725)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5710..5726)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6140..6159)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6259..6281
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6319..6337)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6405..6509
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6510..6578,6579..7370)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6728..6747)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7671..8059
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            8328..8436
                     /note="incP origin of transfer"
     mobile_element  8595..8760
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     terminator      complement(8934..8961)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(9053..9139)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgctactgcc gccaggcaaa ttctgtttta tcagaccgct tctgcgttct gatttaatct
       61 gtatcaggct gaaaatcttc tctcatccgc caaaacagcc aagcttgcat gcctgcaggt
      121 cgactctagt gataaactac cgcattaaag cttgcatgcc tgcaggtctg gacatttttt
      181 tttttttttt tttttttttt ttttwtgtcc gcctttattt gtatagttca tccatgccat
      241 gtgtaatccc agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg
      301 gatctttcga aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc
      361 catcgccaat tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa
      421 tgttgtgtct aattttgaag ttaactttga ttccattctt ttgtttgtct gccatgatgt
      481 atacattgtg tgagttatag ttgtattcca atttgtgtcc aagaatgttt ccatcttctt
      541 taaaatcaat accttttaac tcgattctat taacaagggt atcaccttca aacttgactt
      601 cagcacgtgt cttgtagttc ccgtcatctt tgaaaaatat agttctttcc tgtacataac
      661 cttcgggcat ggcactcttg aaaaagtcat gctgtttcat atgatctggg tatctcgcaa
      721 agcattgaac accataaccg aaagtagtga caagtgttgg ccatggaaca ggtagttttc
      781 cagtagtgca aataaattta agggtaagtt ttccgtatgt tgcatcacct tcaccctctc
      841 cactgacaga aaatttgtgc ccattaacat caccatctaa ttcaacaaga attgggacaa
      901 ctccagtgaa aagttcttct cctttactca ttttttataa cctccttacc tcctgctagc
      961 ccaaaaaaac gggtatggag aaacagtaga gagttgcgat aaaaagcgtc aggtaggatc
     1021 cgctaatctt atggataaaa atgctatggc atagcaaagt gtgacgccgt gcaaataatc
     1081 aatgtggact tttctgccgt gattatagac acttttgtta cgcgtttttg tcatggcttt
     1141 ggtcccgctt tgttacagaa tgcttttaat aagcggggtt accggtttgg ttagcgagaa
     1201 gagccagtaa aagacgcagt gacggcaatg tctgatgcaa tatggacaat tggtttcttc
     1261 tctgaatggc gggagtatga aaagtatggc tgaagcgcaa aatgatcccc tgctgccggg
     1321 atactcgttt aatgcccatc tggtggcggg tttaacgccg attgaggcca acggttatct
     1381 cgattttttt atcgaccgac cgctgggaat gaaaggttat attctcaatc tcaccattcg
     1441 cggtcagggg gtggtgaaaa atcagggacg agaatttgtt tgccgaccgg gtgatatttt
     1501 gctgttcccg ccaggagaga ttcatcacta cggtcgtcat ccggaggctc gcgaatggta
     1561 tcaccagtgg gtttactttc gtccgcgcgc ctactggcat gaatggctta actggccgtc
     1621 aatatttgcc aatacggggt tctttcgccc ggatgaagcg caccagccgc atttcagcga
     1681 cctgtttggg caaatcatta acgccgggca aggggaaggg cgctattcgg agctgctggc
     1741 gataaatctg cttgagcaat tgttactgcg gcgcatggaa gcgattaacg agtcgctcca
     1801 tccaccgatg gataatcggg tacgcgaggc ttgtcagtac atcagcgatc acctggcaga
     1861 cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc ttgtcgccgt cgcgtctgtc
     1921 acatcttttc cgccagcagt tagggattag cgtcttaagc tggcgcgagg accaacgtat
     1981 cagccaggcg aagctgcttt tgagcaccac ccggatgcct atcgccaccg tcggtcgcaa
     2041 tgttggtttt gacgatcaac tctatttctc gcgggtattt aaaaaatgca ccggggccag
     2101 cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat gatgtagccg tcaagttgtc
     2161 ataattggta acgaatcaga caattgacgg cttgacggag tagcataggg tttgcagaat
     2221 ccctgcttcg tccatttgac aggcacatta tgcatcgatg ataagctgtc aaacatgagc
     2281 agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     2341 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     2401 gcagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttaatttgat
     2461 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt
     2521 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     2581 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc
     2641 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag
     2701 ttcggcatgg ggtcaggggg gaccaccgcg ctactgccgc caggcaaatt ctgttttatc
     2761 agaccgcttc tgcgttctga tttaatctgt atcaggctga aaatcttctc tcatccgcca
     2821 aaacagccaa gcttgcatgc ctgcaggtcg actctattac ttgtacagct cgtccatgcc
     2881 gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt
     2941 gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt
     3001 cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag
     3061 cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc
     3121 ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg
     3181 cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac
     3241 cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag
     3301 cttcatgtag tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa
     3361 ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt
     3421 ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg
     3481 gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat
     3541 gttatcctcc tcgcccttgc tcaccatttt ttataacctc cttacctcct gctagcccaa
     3601 aaagacgggt atggagaaac agtagagagt tgcgataaaa agcgtcaggt aggatccgct
     3661 aatcttatgg ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa ataatcaatg
     3721 tggacttttc tgccgtgatt atagacactt ttgttacgcg tttttgtcat ggctttggtc
     3781 ccgctttgtt acagaatgct tttaataagc ggggttaccg gtttggttag cgagaagagc
     3841 cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg gacaattggt ttcttgcttg
     3901 tttcggcgtg ggtatgaatt catgtggctg accgttctgt tgtctctcgc tcttccgagt
     3961 agacgaacaa taaggcctcc ctaacggggg gcctttttta ttgataacaa aagtcagtgc
     4021 ttccgctatt tccaaaatac cgggctaata cggtttaaac gacctcctgg atttgctcag
     4081 acagcctttt cgtcattcgt ttcagccaaa aaacttaaga ccgccggtct tgtccactac
     4141 cttgcagtaa tgcggtggac aggatcggcg gttttctttt ctcttctcaa gaagttccta
     4201 tactttctag agaataggaa cttcggaata ggaacttcct cctgaacggc cataagaacg
     4261 aaggctgtct gttgaactct cgagccgtgg aaacggatga aggcacgaac ccagttgaca
     4321 taagcctgtt cggttcgtaa actgtaatgc aagtagcgta tgcgctcaaa actggatggc
     4381 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg
     4441 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     4501 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4561 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4621 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4681 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4741 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4801 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4861 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4921 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4981 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     5041 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     5101 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     5161 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     5221 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gactcgagca
     5281 tcagcttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
     5341 gtacttcaag atccccaatt cgagctcatc gtccgggccg caagctccta gcggcggatt
     5401 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
     5461 cgactgagcc tttcgtttta tttgatgcct caagctagag agtcattacc ccaggcgttt
     5521 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtcta
     5581 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     5641 atggagttct gaggtcatta ctggatctat caacaggagt ccaagctcag ctaattaagc
     5701 tagcttatcg ataccgtcga cctcgaaccc cacgcccctc tttaatacga cgggcaattt
     5761 gcacttcaga aaatgaagag tttgctttag ccataacaaa agtccagtat gctttttcac
     5821 agcataactg gactgatttc agtttacaac tattctgtct agtttaagac tttattgtca
     5881 tagtttagat ctattttgtt cagtttaaga ctttattgtc cgcccacacc tgcttacgca
     5941 gggcatccat ttattactca accgtaaccg attttgccag gttacgcggc tggtcctcta
     6001 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6061 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     6121 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     6181 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6241 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcgtcaccga
     6301 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6361 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6421 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6481 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6541 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6601 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6661 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6721 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6781 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6841 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6901 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6961 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7021 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7081 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7141 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7201 ataaatctgg agccggtgag cgtgggtccc gcggtatcat tgcagcactg gggccagatg
     7261 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7321 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7381 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7441 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7501 actgagcgtc agaccccgta gaaaagatca aaggcagtta ttggtgcctc actgattaag
     7561 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7621 ttttaatttt tgcggccgca agatccggcc acgatgcgtc cggcgtagag gatctgaaga
     7681 tcagcagttc aacctgttga tagtacgtac taagctctca tgtttcacgt actaagctct
     7741 catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta
     7801 agctctcatg gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa
     7861 caataaaatt aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga
     7921 aaaaaaagaa tatataaggc ttttaaagct tttaaggttt aacggttgtg gacaacaagc
     7981 cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttg agtgacacag
     8041 gaacacttaa cggctgacat gggaattagc catggcatca cagtatcgtg atgacagagg
     8101 cagggagtgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8161 ttcctgcgtt atcaggggat tccttaaggt atactttccg ctgcataacc ctgcttcggg
     8221 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
     8281 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
     8341 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
     8401 gtgctcaacg ggaatcctgc tctgcgaggc tggccgataa gctctgatac cgctcgccgc
     8461 agccgaacga ccgagcgcag cgggtcagtg agcgaggaag cggaagagcg cccaatacgc
     8521 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcgcccaaac ataacaggaa
     8581 gaaaaatgcc ccgctgtggg cggacaaaat agttgggaac tgggaggggt ggaaatggag
     8641 tttttaagga ttatttaggg aagagtgaca aaatagatgg gaactgggtg tagcgtcgta
     8701 agctaatacg aaaattaaaa atgacaaaat agtttggaac tagatttcac ttatctggtt
     8761 cttgaggcgg ttaaaagagc cgtactcttc tccgatgtcg actaggccat gatgctcatt
     8821 ctgtgggacc aaaacgaaaa aacacccttt cgggtgtctt ttctggaatt tggtacgcgg
     8881 atacatattt gaatgtattt agaaaaataa acaaaagagt ttgtagaaac gcaaaaaggc
     8941 catccgtcag gatggccttc tgcttaattt gatgcctggc agtttatggc gggcgtcctg
     9001 cccgccaccc tccgggccgt tgcttcgcaa cgttcaaatc cgctcccggc ggatttgtcc
     9061 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact
     9121 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcatgggg agaccccaca
     9181 ctaccatcgg cgctacggcg tttcacttct gagttcggca tggggtcagg tgggaccacc
     9241 g
//
