LOCUS       sequence-371201-        6711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear envelope labeling with blue fluorescent protein Electra2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, 
            Jia JM, Koster RW, Namikawa K, Piatkevich KD
  TITLE     Dual-expression system for blue fluorescent protein optimization.
  JOURNAL   Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0.
  PUBMED    35715437
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-06-17"; volume: "12(1):10190. doi"; pages: " 
            10.1038/s41598-022-13214-0"
COMMENT     Sequence Label: pElectra2-LaminA
FEATURES             Location/Qualifiers
     source          1..6711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3499..3620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3536..3555)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3590..3609
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3627..4082)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3764..3785)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3976..3995
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4109..4213
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4215..4572
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4215..4406
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4238..4258)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4423..4558
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4607..5401
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4607..5401
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4661..4680)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5271..5290
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5589..5608)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5633..5680
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6009..6597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6498..6517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgtc taagggcgaa gagctgatcg aggaaaatat gcgtatgaag
      661 gtggtcatgg aaggttcggt caacggccac caattcaaat gcacaggtga aggagaaggc
      721 agaccgtacg agggagtgca aaccatgagg atcaaagtca tcgagggagg acccctgcca
      781 tttgcctttg acattcttgc cacgtcgttc ctgttcggca gcaagacctt tatcaagtac
      841 ccggccgaca tccctgattt cttcgaacag tcctttcctg agggctttac ttgggaaaga
      901 gttacgagat acgaagatgg tggagtagtc accgtcacgc aggacaccag ccttgaggat
      961 ggtgggctcg tctacaacgt caaggtcaga ggggtaaact ttcactccaa gggtcccgtg
     1021 atgcagaaga agaccgaggg ttgggagcct ttcacagaga tgatgtatcc agcagatggt
     1081 ggtctgagag gatataccga catcgcactg aaagttgatg gtggtggcca tctgcacgcc
     1141 aacatcgtga caacttacag gtcaaaaaag accgtcggga acatcaagat gcccggtgtc
     1201 catgccgttg attaccgcct ggaaaggatc gaagagagtg acaatgaaac ctacgtagtg
     1261 ctaagagaag tggcagttgc caaatacagc aaccttggtg gtggcatgga cgagctgttc
     1321 aagtccggac tcagatctgg cagcggtgga ggcagcgcat ccggcggaag cggaagcgag
     1381 accccgtccc agcggcgcgc cacccgcagc ggggcgcagg ccagctccac tccgctgtcg
     1441 cccacccgca tcacccggct gcaggagaag gaggacctgc aggagctcaa tgatcgcttg
     1501 gcggtctaca tcgaccgtgt gcgctcgctg gaaacggaga acgcagggct gcgccttcgc
     1561 atcaccgagt ctgaagaggt ggtcagccgc gaggtgtccg gcatcaaggc cgcctacgag
     1621 gccgagctcg gggatgcccg caagaccctt gactcagtag ccaaggagcg cgcccgcctg
     1681 cagctggagc tgagcaaagt gcgtgaggag tttaaggagc tgaaagcgcg caataccaag
     1741 aaggagggtg acctgatagc tgctcaggct cggctgaagg acctggaggc tctgctgaac
     1801 tccaaggagg ccgcactgag cactgctctc agtgagaagc gcacgctgga gggcgagctg
     1861 catgatctgc ggggccaggt ggccaagctt gaggcagccc taggtgaggc caagaagcaa
     1921 cttcaggatg agatgctgcg gcgggtggat gctgagaaca ggctgcagac catgaaggag
     1981 gaactggact tccagaagaa catctacagt gaggagctgc gtgagaccaa gcgccgtcat
     2041 gagacccgac tggtggagat tgacaatggg aagcagcgtg agtttgagag ccggctggcg
     2101 gatgcgctgc aggaactgcg ggcccagcat gaggaccagg tggagcagta taagaaggag
     2161 ctggagaaga cttattctgc caagctggac aatgccaggc agtctgctga gaggaacagc
     2221 aacctggtgg gggctgccca cgaggagctg cagcagtcgc gcatccgcat cgacagcctc
     2281 tctgcccagc tcagccagct ccagaagcag ctggcagcca aggaggcgaa gcttcgagac
     2341 ctggaggact cactggcccg tgagcgggac accagccggc ggctgctggc ggaaaaggag
     2401 cgggagatgg ccgagatgcg ggcaaggatg cagcagcagc tggacgagta ccaggagctt
     2461 ctggacatca agctggccct ggacatggag atccacgcct accgcaagct cttggagggc
     2521 gaggaggaga ggctacgcct gtcccccagc cctacctcgc agcgcagccg tggccgtgct
     2581 tcctctcact catcccagac acagggtggg ggcagcgtca ccaaaaagcg caaactggag
     2641 tccactgaga gccgcagcag cttctcacag cacgcacgca ctagcgggcg cgtggccgtg
     2701 gaggaggtgg atgaggaggg caagtttgtc cggctgcgca acaagtccaa tgaggaccag
     2761 tccatgggca attggcagat caagcgccag aatggagatg atcccttgct gacttaccgg
     2821 ttcccaccaa agttcaccct gaaggctggg caggtggtga cgatctgggc tgcaggagct
     2881 ggggccaccc acagcccccc taccgacctg gtgtggaagg cacagaacac ctggggctgc
     2941 gggaacagcc tgcgtacggc tctcatcaac tccactgggg aagaagtggc catgcgcaag
     3001 ctggtgcgct cagtgactgt ggttgaggac gacgaggatg aggatggaga tgacctgctc
     3061 catcaccacc acggctccca ctgcagcagc tcgggggacc ccgctgagta caacctgcgc
     3121 tcgcgcaccg tgctgtgcgg gacctgcggg cagcctgccg acaaggcatc tgccagcggc
     3181 tcaggagccc aggtgggcgg acccatctcc tctggctctt ctgcctccag tgtcacggtc
     3241 actcgcagct accgcagtgt ggggggcagt gggggtggca gcttcgggga caatctggtc
     3301 acccgctcct acctcctggg caactccagc ccccgaaccc agagccccca gaactgcagc
     3361 atcatgtaag gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     3421 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3481 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3541 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3601 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     3661 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3721 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3781 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3841 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3901 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3961 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     4021 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     4081 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4141 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4201 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     4261 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4321 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4381 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4441 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4501 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4561 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     4621 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4681 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4741 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4801 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4861 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4921 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4981 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     5041 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5101 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     5161 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5221 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5281 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5341 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5401 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     5461 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5521 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     5581 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     5641 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     5701 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5761 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5821 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     5881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6001 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6061 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6121 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6661 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
