LOCUS       sequence-371213-        9609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Third generation lentiviral vector expressing Kinesin Heavy Chain 5 
            isoform B Rigor mutant (T92N) fused to Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Zamponi E, Meehl JB, Voeltz GK
  TITLE     The ER ladder is a unique morphological feature of developing 
            mammalian axons.
  JOURNAL   Dev Cell. 2022 Jun 6;57(11):1369-1382.e6. doi: 
            10.1016/j.devcel.2022.05.002. Epub 2022 May 23.
  PUBMED    35609616
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2022.05.002"; journalName: "Dev Cell"; date: 
            "2022-06-6- 6"; volume: "57"; issue: "11"; pages: "1369-1382.e6"
COMMENT     Sequence Label: pLV-Kif5b-T92N-Myc
FEATURES             Location/Qualifiers
     source          1..9609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..78
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     104..128
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3073..3102
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    3112..3700
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3165..3185)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3583..3594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3787..4020
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4092..4213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4129..4148)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4183..4202
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4253..4388
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4408..4427)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4409..4427)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4437..4454)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4437..4453)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4446..4468)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4595..5050
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4682..4701)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4892..4913
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5076..5180
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5181..5249,5250..6041)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5399..5418)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6212..6800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6701..6720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6954..6971
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7088..7109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7124..7129,7130..7147,7148..7154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7162..7178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7167..7189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7186..7202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7186..7202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7221..7241
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7223..7241
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7269..7495
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7496..7676
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7723..7848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8341..8574
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8759..8803
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9070..9186
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9209..9512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(9513..9609,1..107)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
       61 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      121 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct
      181 agaatggcgg acctggccga gtgcaacatc aaagtgatgt gtcgcttcag acctctcaac
      241 gagtctgaag tgaaccgcgg cgacaagtac atcgccaagt ttcagggaga agacacggtc
      301 gtgatcgcgt ccaagcctta tgcatttgat cgggtgttcc agtcaagcac atctcaagag
      361 caagtgtata atgactgtgc aaagaagatt gttaaagatg tacttgaagg atataatgga
      421 acaatatttg catatggaca aacatcctct gggaagaacc acacaatgga gggtaaactt
      481 catgatccag aaggcatggg aattattcca agaatagtgc aagatatttt taattatatt
      541 tactccatgg atgaaaattt ggaatttcat attaaggttt catattttga aatatatttg
      601 gataagataa gggacctgtt agatgtttca aagaccaacc tttcagttca tgaagacaaa
      661 aaccgagttc cctatgtaaa ggggtgcaca gagcgttttg tatgtagtcc agatgaagtt
      721 atggatacca tagatgaagg aaaatccaac agacatgtag cagttacaaa tatgaatgaa
      781 catagctcta ggagtcacag tatatttctt attaatgtca aacaagagaa cacacaaacg
      841 gaacaaaagc tgagtggaaa actttatctg gttgatttag ctggtagtga aaaggttagt
      901 aaaactggag ctgaaggtgc tgtgctggat gaagctaaaa acatcaacaa gtcactttct
      961 gctcttggaa atgttatttc tgctttggct gagggtagta catatgttcc atatcgagat
     1021 agtaaaatga caagaatcct tcaagattca ttaggtggca actgtagaac cactattgta
     1081 atttgctgct ctccatcatc atacaatgag tctgaaacaa aatctacact cttatttggc
     1141 caaagggcca aaacaattaa gaacacagtt tgtgtcaatg tggagttaac tgcagaacag
     1201 tggaaaaaga agtatgaaaa agaaaaagaa aaaaataaga tcctgcggaa cactattcag
     1261 tggcttgaaa atgagctcaa cagatggcgt aatggggaga cggtgcctat tgatgaacag
     1321 tttgacaaag agaaagccaa cttggaagct ttcacagtgg ataaagatat tactcttacc
     1381 aatgataaac cagcaaccgc aattggagtt ataggaaatt ttactgatgc tgaaagaaga
     1441 aagtgtgaag aagaaattgc taaattatac aaacagcttg atgacaagga tgaagaaatt
     1501 aaccagcaaa gtcaactggt agagaaactg aagacgcaaa tgttggatca ggaggagctt
     1561 ttggcatcta ccagaaggga tcaagacaat atgcaagctg agctgaatcg ccttcaagca
     1621 gaaaatgatg cctctaaaga agaagtgaaa gaagttttac aggccctaga agaacttgct
     1681 gtcaattatg atcagaagtc tcaggaagtt gaagacaaaa ctaaggaata tgaattgctt
     1741 agtgatgaat tgaatcagaa atcggcaact ttagcgagta tagatgctga gcttcagaaa
     1801 cttaaggaaa tgaccaacca ccagaaaaaa cgagcagctg agatgatggc atctttacta
     1861 aaagaccttg cagaaatagg aattgctgtg ggaaataatg atgtaaagca gcctgaggga
     1921 actggcatga tagatgaaga gttcactgtt gcaagactct acattagcaa aatgaagtca
     1981 gaagtaaaaa ccatggtgaa acgttgcaag cagttagaaa gcacacaaac tgagagcaac
     2041 aaaaaaatgg aagaaaatga aaaggagtta gcagcatgtc agcttcgtat ctctcaacat
     2101 gaagccaaaa tcaagtcatt gactgaatac cttcaaaatg tggaacaaaa gaaaagacag
     2161 ttggaggaat ctgtcgatgc cctcagtgaa gaactagtcc agcttcgagc acaagagaaa
     2221 gtccatgaaa tggaaaagga gcacttaaat aaggttcaga ctgcaaatga agttaagcaa
     2281 gctgttgaac agcagatcca gagccataga gaaactcatc aaaaacagat cagtagtttg
     2341 agagatgaag tagaagcaaa agcaaaactt attactgatc ttcaagacca aaaccagaaa
     2401 atgatgttag agcaggaacg tctaagagta gaacatgaga agttgaaagc cacagatcag
     2461 gaaaagagca gaaaactaca tgaacttacg gttatgcaag atagacgaga acaagcaaga
     2521 caagacttga agggtttgga agagacagtg gcaaaagaac ttcagacttt acacaacctg
     2581 cgcaaactct ttgttcagga cctggctaca agagttaaaa agagtgctga gattgattct
     2641 gatgacaccg gaggcagcgc tgctcagaag caaaaaatct cctttcttga aaataatctt
     2701 gaacagctca ctaaagtgca caaacagttg gtacgtgata atgcagatct ccgctgtgaa
     2761 cttcctaagt tggaaaagcg acttcgagct acagctgaga gagtgaaagc tttggaatca
     2821 gcactgaaag aagctaaaga aaatgcatct cgtgatcgca aacgctatca gcaagaagta
     2881 gatcgcataa aggaagcagt caggtcaaag aatatggcca gaagagggca ttctgcacag
     2941 attgctaaac ctattcgtcc cgggcaacat ccagcagctt ctccaactca cccaagtgca
     3001 attcgtggag gaggtgcatt tgttcagaac agccagccag tggcagtgcg aggtggagga
     3061 ggcaaacaag tggaacaaaa acttatttct gaagaagatc tgtaagtcga caatcaacct
     3121 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3181 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3241 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3301 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3361 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3421 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3481 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     3541 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3601 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3661 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac
     3721 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3781 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3841 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3901 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3961 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4021 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     4081 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4141 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4201 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     4261 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4321 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4381 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     4441 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4501 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4561 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     4621 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4681 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4741 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4801 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4861 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4921 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4981 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5041 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5101 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5161 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5221 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5281 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5341 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5401 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5461 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5521 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5581 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5641 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5701 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5761 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5821 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5881 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5941 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6001 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6061 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6121 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6181 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6241 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6301 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6361 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6421 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6481 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6541 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6601 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6661 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6721 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6781 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6841 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6901 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6961 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7021 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7081 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttccac tttatgcttc
     7141 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     7201 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     7261 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     7321 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     7381 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     7441 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     7501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     7681 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     7741 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     7801 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     7861 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     7921 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     7981 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     8041 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     8101 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     8161 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     8221 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     8281 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     8341 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     8401 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     8461 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     8521 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     8581 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8641 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     8701 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8761 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     8821 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     8881 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     8941 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     9001 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     9061 tcggttaact tttaaaagaa aaggggggat tggggggtat agtgcagggg aaagaatagt
     9121 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa
     9181 aattttatcg ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     9241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     9301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     9361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     9421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     9481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     9541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     9601 ggagtttgt
//
