LOCUS       sequence-371320-        9001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a biosensor comprised of a fast-maturing pH-stable red fluorescent 
            protein fused to a pH-sensitive green fluorescent protein variant. 
            Cytosolic localization..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Rosado CJ, Mijaljica D, Hatzinisiriou I, Prescott M, Devenish RJ
  TITLE     Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover 
            of cytosol and organelles in yeast.
  JOURNAL   Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
  PUBMED    18094608
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2008-02"; volume: "4(2)"; pages: "205-13. Epub 
            2007 Nov 21"
COMMENT     Sequence Label: pAS1NB c Rosella I
FEATURES             Location/Qualifiers
     source          1..9001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(244..514)
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     CDS             complement(630..1337)
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKANF
                     KIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEFV
                     TAAGITQGMDELYK"
     primer_bind     complement(670..690)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     1285..1313
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(1368..2042)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGRFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(1427..1450)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1625..1644)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1842..1862
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1894..1912
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2039..2048
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2058..2110
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(3552..3569)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3552..3568)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3561..3583)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4005..5351
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(5489..6583)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(6584..6989)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(7009..7027)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7095..7199
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7200..7268,7269..8060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7418..7437)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8231..8819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8720..8739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8973..8990
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttta
      241 acgaacgcag aattttcgag ttattaaact taaaatacgc tgaacccgaa catagaaata
      301 tcgaatggga aaaaaaaact ggataaaggc attaaaagag gagcgaattt ttttttaata
      361 aaaatcttaa taatcattaa aagataaata atagtctata tatacgtata taaataaaaa
      421 atattcaaaa aataaaataa actattattt tagcgtaaag gatggggaaa gagaaaagaa
      481 aaaaattgat ctatcgattt caattcaatt caatttattt cttttcggat aagaaagcaa
      541 cacctggcaa ttccttacct tccaataatt ccaaagaagc accaccacca gtagagacat
      601 gggagatccg cggccgctca gtgatcagat ttgtatagtt catccatgcc ttgtgtaatc
      661 ccagcagctg ttacaaactc aagaaggacc atgtggtctc tcttttcgtt gggatctttc
      721 gaaagagtag aagttgtaaa caggtaatgg ttgtctggta aaaggacggg cccatcgcca
      781 attggagtat tttgttgata atggtctgct agttgaacgc ctccatcttc aatgttgtgt
      841 ctaattttga agttagcttt gattccattc ttttgtttgt ctgccatgat gtacacctgg
      901 tgatcgttat agttgtattc caatttgtgt ccaagaatgt ttccatcttc tttaaaatca
      961 atacctttta actcgattct attaacaagg gtatcacctt caaacttgac ttcagcacgt
     1021 gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt cctgtacata accttcgggc
     1081 atggcactct tgaaaaagtc atgccgtttc atatgatctg ggtatcttga aaagcattga
     1141 acaccataag ttaaagtagt gacaagtgtt ggccaaggaa caggtagttt tccagtagtg
     1201 caaataaatt taagggtaag ttttccgtat gttgcatcac cttcaccctc tccactgaca
     1261 gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac aactccagtg
     1321 aaaagttctt ctccttttag atctcccgaa gaagaccagg gatcagacag gaacaggtgg
     1381 tggcggccct cggtgcgctc gtactgctcc acgatggtgt agtcctcgtt gtgggaggtg
     1441 atgtccagct tggagtccac gtagtagtag ccgggcagct gcacgggctt cttggccatg
     1501 tagatggact tgaactccac caggtagtgg ccgccgtcct tcagcttcag ggccttgtgg
     1561 atctcgccct tcagcacgcc gtcgcggggg tacaggcgct cggtggaggg ctcccagccc
     1621 atagtcttct tctgcattac ggggccgtcg gaggggaagt tcacgccgat gaacttcacc
     1681 ttgtagatga agcggccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg
     1741 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcttgtag
     1801 tcggggatgt cggcggggtg cttcacgtac accttggagc cgtactggaa ctggggggac
     1861 aggatgtccc aggcgaaggg cagggggccg cccttggtca ccttcagctt ggcggtctgg
     1921 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg
     1981 gagccctcca tgcgcacctt gaagcgcatg aactccttga tgacgtcctc ggaggaggcc
     2041 atggtggcga ctagtgggga tcccgggccc gcggtaccgt cgactgcaga attcgaagct
     2101 tgagctcgag acatgtttag atcttctaga gaaaaagtag ataattactt ccttgatgat
     2161 ctgtaaaaaa gagaaaaaga aagcatctaa gaacttgaaa aactacgaat tagaaaagac
     2221 caaatatgta tttcttgcat tgaccaattt atgcaagttt atatatatgt aaatgtaagt
     2281 ttcacgaggt tctactaaac taaaccaccc ccttggttag aagaaaagag tgtgtgagaa
     2341 caggctgttg ttgtcacacg attcggacaa ttctgtttga aagagagaga gtaacagtac
     2401 gatcgaacga actttgctct ggagatcaca gtgggcatca tagcatgtgg tactaaaccc
     2461 tttcccgcca ttccagaacc ttcgattgct tgttacaaaa cctgtgagcc gtcgctagga
     2521 ccttgttgtg tgacgaaatt ggaagctgca atcaatagga agacaggaag tcgagcgtgt
     2581 ctgggttttt tcagttttgt tctttttgca aacaaatcac gagcgacggt aatttctttc
     2641 tcgataagag gccacgtgct ttatgagggt aacatcaatt caagaaggag ggaaacactt
     2701 cctttttctg gccctgataa tagtatgagg gtgaagccaa aataaaggat tcgcgcccaa
     2761 atcggcatct ttaaatgcag gtatgcgata gttcctcact ctttccttac tcacgagtaa
     2821 ttcttgcaaa tgcctattgt gcagatgtta taatatctgt gcgtcttgag ttgaagtcag
     2881 gaatctaaaa taaaaattaa ggttaataaa aagaggaaag aaaaaaaaat taatcgattt
     2941 acagaaactt gcacactaaa aatacacaac taaaagcaat tacagtatgg gaagtcatcg
     3001 acgttatctc tactatagta tattatcatt tctattatta tcctgctcag tggtacttgc
     3061 aaaacaagat gagaccccat tctttgaagg tacttcttcg aaaaattcgc gtctaactgc
     3121 acaagataag ggcaatgata cgtgcccgcc atgttttaat tgtatgctac ctatttttga
     3181 atgcaaacag ttttctgaat gcaattcgta cactggtaga tgtgagtgta tagaagggtt
     3241 tgcaggtgat gattgctctc tgcccctctg tggcggtcta tcaccggatg aaagcggtaa
     3301 taaggatcgt cccataagag cacaaaatga cacctgtcat tgtgataacg gatggggagg
     3361 gatcaattgt gacgtttgtc aagaagattt tgtctgtgat gcgttcatgc ctgatcctag
     3421 tattaagggg acatgttata agaatggtat gattgtagat aaagtatttt caggttgtaa
     3481 tgtgaccaat gagaaaattc tacagctcta gagtcgactc tagagaatcc ccgggtaccg
     3541 agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3601 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     3661 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     3721 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tttatcggat cgtacttgtt
     3781 acccatcatt gaattttgaa catccgaacc tgggagtttt ccctgaaaca gatagtatat
     3841 ttgaacctgt ataataatat atagtctagc gctttacgga agacaatgta tgtatttcgg
     3901 ttcctggaga aactattgca tctattgcat aggtaatctt gcacgtcgca tccccggttc
     3961 attttctgcg tttccatctt gcacttcaat agcatatctt tgttaacgaa gcatctgtgc
     4021 ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
     4081 gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct
     4141 gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
     4201 tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag
     4261 aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa
     4321 agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact
     4381 ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc
     4441 cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc
     4501 attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact
     4561 ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt
     4621 tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc
     4681 gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat
     4741 aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg
     4801 gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt
     4861 ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt
     4921 ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac
     4981 tttctagcta gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc
     5041 gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata
     5101 tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct
     5161 taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt
     5221 gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt
     5281 tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt
     5341 gatattggat cgatccgatg ataagctgtc aaacatgaga attaattcta ccctatgaac
     5401 atattccatt ttgtaatttc gtgtcgtttc tattatgaat ttcatttata aagtttatgt
     5461 acaaatatca taaaaaaaga gaatcttttt aagcaaggat tttcttaact tcttcggcga
     5521 cagcatcacc gacttcggtg gtactgttgg aaccacctaa atcaccagtt ctgatacctg
     5581 catccaaaac ctttttaact gcatcttcaa tggccttacc ttcttcaggc aagttcaatg
     5641 acaatttcaa catcattgca gcagacaaga tagtggcgat agggttgacc ttattctttg
     5701 gcaaatctgg agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg
     5761 gcaaagaggc caaggacgca gatggcaaca aacccaagga acctgggata acggaggctt
     5821 catcggagat gatatcacca aacatgttgc tggtgattat aataccattt aggtgggttg
     5881 ggttcttaac taggatcatg gcggcagaat caatcaattg atgttgaacc ttcaatgtag
     5941 gaaattcgtt cttgatggtt tcctccacag tttttctcca taatcttgaa gaggccaaaa
     6001 cattagcttt atccaaggac caaataggca atggtggctc atgttgtagg gccatgaaag
     6061 cggccattct tgtgattctt tgcacttctg gaacggtgta ttgttcacta tcccaagcga
     6121 caccatcacc atcgtcttcc tttctcttac caaagtaaat acctcccact aattctctga
     6181 caacaacgaa gtcagtacct ttagcaaatt gtggcttgat tggagataag tctaaaagag
     6241 agtcggatgc aaagttacat ggtcttaagt tggcgtacaa ttgaagttct ttacggattt
     6301 ttagtaaacc ttgttcaggt ctaacactac ctgtacccca tttaggacca cccacagcac
     6361 ctaacaaaac ggcatcagcc ttcttggagg cttccagcgc ctcatctgga agtggaacac
     6421 ctgtagcatc gatagcagca ccaccaatta aatgattttc gaaatcgaac ttgacattgg
     6481 aacgaacatc agaaatagct ttaagaacct taatggcttc ggctgtgatt tcttgaccaa
     6541 cgtggtcacc tggcaaaacg acgatcttct taggggcaga cattagaatg gtatatcctt
     6601 gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat
     6661 tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa cttcaagtat
     6721 tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc cggttccggc
     6781 gctctcacct ttcctttttc tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga
     6841 cctctgaaat taacaaaaaa tttccagtca tcgaatttga ttctgtgcga tagcgcccct
     6901 gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg aactcttgca
     6961 tcttacgata cctgagtatt cccacagtta attcttgaag acgaaagggc ctcgtgatac
     7021 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7081 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7141 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7201 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7261 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7321 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7381 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7441 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7501 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7561 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7621 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7681 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7741 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7801 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7861 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7921 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7981 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8041 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8101 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8161 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8221 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8281 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8341 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8401 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8461 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8521 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8581 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8641 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8701 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8761 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8821 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8881 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8941 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9001 a
//
