LOCUS       sequence-37143-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Ramaswamy S, Nakamura N, Vazquez F, Batt DB, Perera S, Roberts TM, 
            Sellers WR
  TITLE     Regulation of G1 progression by the PTEN tumor suppressor protein is
            linked to inhibition of the phosphatidylinositol 3-kinase/Akt 
            pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5.
  PUBMED    10051603
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1999 Mar 2. 96(5):2110-5."
COMMENT     Sequence Label: 901 pLNCX myr HA Akt1
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             108..149
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             168..194
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc gcggccccaa gcttaccatg gggtcttcaa
      121 aatctaaacc aaaggacccc agccagcgcc ggcgcaggat ccgaggttac ccatacgacg
      181 tgccagatta cgccagcgac gtggctattg tgaaggaggg ttggctgcac aaacgagggg
      241 agtacatcaa gacctggcgg ccacgctact tcctcctcaa gaatgatggc accttcattg
      301 gctacaagga gcggccgcag gatgtggacc aacgtgaggc tcccctcaac aacttctctg
      361 tggcgcagtg ccagctgatg aagacggagc ggccccggcc caacaccttc atcatccgct
      421 gcctgcagtg gaccactgtc atcgaacgca ccttccatgt ggagactcct gaggagcggg
      481 aggagtggac aaccgccatc cagactgtgg ctgacggcct caagaagcag gaggaggagg
      541 agatggactt ccggtcgggc tcacccagtg acaactcagg ggctgaagag atggaggtgt
      601 ccctggccaa gcccaagcac cgcgtgacca tgaacgagtt tgagtacctg aagctgctgg
      661 gcaagggcac tttcggcaag gtgatcctgg tgaaggagaa ggccacaggc cgctactacg
      721 ccatgaagat cctcaagaag gaagtcatcg tagccaagga cgaagtggcc cacacactca
      781 ccgagaaccg cgtcctgcag aactccaggc accccttcct cacagccctg aagtactctt
      841 tccagaccca cgaccgcctc tgctttgtca tggagtacgc caacgggggc gagctgttct
      901 tccacctgtc ccgggagcgt gtgttctccg
//
