LOCUS       sequence-371587-       10243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector with a ubiquitin promoter expressing GFP, human 
            AKT3 transcript variant 1 ORF with a phosphodeficient mutation at 
            Serine 472, and Cre recombinase, separated by T2A motifs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10243)
  TITLE     Retroviral vectors for expression of AKT kinases with GFP and Cre 
            recombinase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRubiG-T2A-AKT3(S472A)-T2A-Cre
FEATURES             Location/Qualifiers
     source          1..10243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     79..98
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(179..196)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(350..938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(430..449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1109..1900,1901..1969))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1732..1751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1970..2074)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2142..2160
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        2260..2639
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2640..2843
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2794..2814
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2844..3019
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    3082..3281
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3482..3898
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3828..3850
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3866..3882
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        3969..5180
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5017..5036
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5228..5237
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5234..5236,5237..5239,5240..5950)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5234..5236,5237..5239,5240..5950)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5540..5559)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5887..5908
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5951..6004
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7445..7498
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7505..8533
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKCFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    8557..9145
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8610..8630)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9028..9039)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9148..9164)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9149..9165)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9280..9705
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(9808..9830)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9966..9986)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(9971..10243,1..57)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10151..10243,1..43)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
       61 cttactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
      121 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
      181 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      241 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      301 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      361 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      421 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      481 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      541 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      601 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      661 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      721 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      781 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      841 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      901 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      961 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1021 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1081 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1141 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1201 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1261 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1321 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1381 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     1441 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1501 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1561 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1621 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1681 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     1741 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1801 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1861 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1921 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1981 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2041 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2101 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2161 cctttcgtct tcaagaatta gcttggccat tgcatacgtt gtatccatat cataatatgt
     2221 acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt
     2281 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     2341 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     2401 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     2461 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     2521 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2581 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     2641 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     2701 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     2761 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     2821 gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact cggcgcgcca
     2881 gtcttccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct cttgctgttt
     2941 gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     3001 acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg cccagggacc
     3061 accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt ccgattgtct
     3121 agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact agctctgtat
     3181 ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg
     3241 tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt cgatgtggaa
     3301 tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc
     3361 tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg
     3421 ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta
     3481 gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag atgtcgagcg
     3541 gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct tctgctctgc
     3601 agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc gagacctcat
     3661 cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag accaggtccc
     3721 ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca agccctttgt
     3781 acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc ttgaacctcc
     3841 tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc taggcgccgg
     3901 aattgaagat ctcacacagc ggccgcacac acttcgaacc ttaattaacc cgtgtcggct
     3961 ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
     4021 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
     4081 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
     4141 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
     4201 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
     4261 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
     4321 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
     4381 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
     4441 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
     4501 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
     4561 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
     4621 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg
     4681 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
     4741 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
     4801 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
     4861 taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
     4921 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     4981 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     5041 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     5101 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     5161 ttttggcttt tttgttagac gaagcttggg ctgcaggtcg actctagagg atccccgggt
     5221 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     5281 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     5341 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     5401 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     5461 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     5521 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     5581 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     5641 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     5701 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     5761 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     5821 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     5881 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     5941 gctgtacaag gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     6001 ccctagtaca agcgatgtta ccattgtgaa agaaggttgg gttcagaaga ggggagaata
     6061 tataaaaaac tggaggccaa gatacttcct tttgaagaca gatggctcat tcataggata
     6121 taaagagaaa cctcaagatg tggatttacc ttatcccctc aacaactttt cagtggcaaa
     6181 atgccagtta atgaaaacag aacgaccaaa gccaaacaca tttataatca gatgtctcca
     6241 gtggactact gttatagaga gaacatttca tgtagatact ccagaggaaa gggaagaatg
     6301 gacagaagct atccaggctg tagcagacag actgcagagg caagaagagg agagaatgaa
     6361 ttgtagtcca acttcacaaa ttgataatat aggagaggaa gagatggatg cctctacaac
     6421 ccatcataaa agaaagacaa tgaatgattt tgactatttg aaactactag gtaaaggcac
     6481 ttttgggaaa gttattttgg ttcgagagaa ggcaagtgga aaatactatg ctatgaagat
     6541 tctgaagaaa gaagtcatta ttgcaaagga tgaagtggca cacactctaa ctgaaagcag
     6601 agtattaaag aacactagac atcccttttt aacatccttg aaatattcct tccagacaaa
     6661 agaccgtttg tgttttgtga tggaatatgt taatgggggc gagctgtttt tccatttgtc
     6721 gagagagcgg gtgttctctg aggaccgcac acgtttctat ggtgcagaaa ttgtctctgc
     6781 cttggactat ctacattccg gaaagattgt gtaccgtgat ctcaagttgg agaatctaat
     6841 gctggacaaa gatggccaca taaaaattac agattttgga ctttgcaaag aagggatcac
     6901 agatgcagcc accatgaaga cattctgtgg cactccagaa tatctggcac cagaggtgtt
     6961 agaagataat gactatggcc gagcagtaga ctggtggggc ctaggggttg tcatgtatga
     7021 aatgatgtgt gggaggttac ctttctacaa ccaggaccat gagaaacttt ttgaattaat
     7081 attaatggaa gacattaaat ttcctcgaac actctcttca gatgcaaaat cattgctttc
     7141 agggctcttg ataaaggatc caaataaacg ccttggtgga ggaccagatg atgcaaaaga
     7201 aattatgaga cacagtttct tctctggagt aaactggcaa gatgtatatg ataaaaagct
     7261 tgtacctcct tttaaacctc aagtaacatc tgagacagat actagatatt ttgatgaaga
     7321 atttacagct cagactatta caataacacc acctgaaaaa tatgatgagg atggtatgga
     7381 ctgcatggac aatgagaggc ggccgcattt ccctcaattt gcctactctg caagtggacg
     7441 agaagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag
     7501 tacatccaat ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag
     7561 tgatgaggtt cgcaagaacc tgatggacat gttcagggat cgccaggcgt tttctgagca
     7621 tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa
     7681 ccggaaatgc tttcccgcag aacctgaaga tgttcgcgat tatcttctat atcttcaggc
     7741 gcgcggtctg gcagtaaaaa ctatccagca acatttgggc cagctaaaca tgcttcatcg
     7801 tcggtccggg ctgccacgac caagtgacag caatgctgtt tcactggtta tgcggcggat
     7861 ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac
     7921 tgatttcgac caggttcgtt cactcatgga aaatagcgat cgctgccagg atatacgtaa
     7981 tctggcattt ctggggattg cttataacac cctgttacgt atagccgaaa ttgccaggat
     8041 cagggttaaa gatatctcac gtactgacgg tgggagaatg ttaatccata ttggcagaac
     8101 gaaaacgctg gttagcaccg caggtgtaga gaaggcactt agcctggggg taactaaact
     8161 ggtcgagcga tggatttccg tctctggtgt agctgatgat ccgaataact acctgttttg
     8221 ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc agccagctat caactcgcgc
     8281 cctggaaggg atttttgaag caactcatcg attgatttac ggcgctaagg atgactctgg
     8341 tcagagatac ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc
     8401 ccgcgctgga gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat
     8461 tgtcatgaac tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga
     8521 agatggcgat tagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     8581 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8641 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     8701 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     8761 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8821 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8881 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     8941 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     9001 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     9061 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     9121 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagatat cagtggtcca
     9181 ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat
     9241 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
     9301 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag
     9361 aatagagaag ttcagatcaa ggtcaggaac agatggaaca gggtcgaccc tagagaacca
     9421 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     9481 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     9541 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
     9601 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     9661 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg
     9721 gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggtaag ctggctgcct
     9781 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     9841 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     9901 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtagatccg
     9961 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
    10021 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
    10081 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
    10141 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
    10201 actaattttt tttatttatg cagaggccga ggccgcctcg gcc
//
