LOCUS       sequence-371597-       10246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector with a ubiquitin promoter expressing GFP, human 
            AKT1 ORF, and Cre recombinase, separated by T2A motifs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10246)
  TITLE     Retroviral vectors for expression of AKT kinases with GFP and Cre 
            recombinase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRubiG-T2A-AKT1-T2A-Cre
FEATURES             Location/Qualifiers
     source          1..10246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    90..289
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             490..906
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     836..858
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     874..890
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        977..2188
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2025..2044
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      2236..2245
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2242..2244,2245..2247,2248..2958)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2242..2244,2245..2247,2248..2958)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2548..2567)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2895..2916
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2959..3012
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3019..4455
                     /codon_start=1
                     /gene="AKT1"
                     /product="human serine/threonine protein kinase activated 
                     by growth factors"
                     /label=Akt1
                     /translation="SDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVD
                     QREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQT
                     VADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKV
                     ILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRL
                     CFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLML
                     DKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYE
                     MMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAK
                     EIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSME
                     CVDSERRPHFPQFSYSASGTA"
     CDS             4456..4509
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4516..5544
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKCFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    5568..6156
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5621..5641)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6039..6050)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6159..6175)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6160..6176)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6291..6716
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6819..6841)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6977..6997)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6982..7311
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7162..7297
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7333..7352
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7433..7450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7604..8192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7684..7703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8363..9154,9155..9223))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8986..9005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9224..9328)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9396..9414
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        9514..9893
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9894..10097
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10048..10068
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             join(10098..10246,1..27)
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
ORIGIN
        1 gactacccac gacgggggtc tttcatttgg gggctcgtcc gggatttgga gacccctgcc
       61 cagggaccac cgacccacca ccgggaggta agctggccag caacttatct gtgtctgtcc
      121 gattgtctag tgtctatgtt tgatgttatg cgcctgcgtc tgtactagtt agctaactag
      181 ctctgtatct ggcggacccg tggtggaact gacgagttct gaacacccgg ccgcaaccct
      241 gggagacgtc ccagggactt tgggggccgt ttttgtggcc cgacctgagg aagggagtcg
      301 atgtggaatc cgaccccgtc aggatatgtg gttctggtag gagacgagaa cctaaaacag
      361 ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc gaagccgcgc gtcttgtctg
      421 ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct
      481 gaaaattagg gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat
      541 gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc
      601 tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga
      661 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac
      721 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag
      781 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
      841 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
      901 ggcgccggaa ttgaagatct cacacagcgg ccgcacacac ttcgaacctt aattaacccg
      961 tgtcggctcc agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     1021 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     1081 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     1141 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     1201 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     1261 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     1321 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     1381 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     1441 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     1501 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
     1561 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     1621 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     1681 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     1741 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     1801 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     1861 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     1921 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     1981 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     2041 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     2101 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     2161 ctggccgttt ttggcttttt tgttagacga agcttgggct gcaggtcgac tctagaggat
     2221 ccccgggtac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2281 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2341 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2401 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2461 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2521 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2581 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2641 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2701 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2761 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2821 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2881 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2941 atggacgagc tgtacaagga gggcagagga agtcttctaa catgcggtga cgtggaggag
     3001 aatcccggcc ctagtacaag cgacgtggct attgtgaagg agggttggct gcacaaacga
     3061 ggggagtaca tcaagacctg gcggccacgc tacttcctcc tcaagaatga tggcaccttc
     3121 attggctaca aggagcggcc gcaggatgtg gaccaacgtg aggctcccct caacaacttc
     3181 tctgtggcgc agtgccagct gatgaagacg gagcggcccc ggcccaacac cttcatcatc
     3241 cgctgcctgc agtggaccac tgtcatcgaa cgcaccttcc atgtggagac tcctgaggag
     3301 cgggaggagt ggacaaccgc catccagact gtggctgacg gcctcaagaa gcaggaggag
     3361 gaggagatgg acttccggtc gggctcaccc agtgacaact caggggctga agagatggag
     3421 gtgtccctgg ccaagcccaa gcaccgcgtg accatgaacg agtttgagta cctgaagctg
     3481 ctgggcaagg gcactttcgg caaggtgatc ctggtgaagg agaaggccac aggccgctac
     3541 tacgccatga agatcctcaa gaaggaagtc atcgtggcca aggacgaggt ggcccacaca
     3601 ctcaccgaga accgcgtcct gcagaactcc aggcacccct tcctcacagc cctgaagtac
     3661 tctttccaga cccacgaccg cctctgcttt gtcatggagt acgccaacgg gggcgagctg
     3721 ttcttccacc tgtcccggga gcgtgtgttc tccgaggacc gggcccgctt ctatggcgct
     3781 gagattgtgt cagccctgga ctacctgcac tcggagaaga acgtggtgta ccgggacctc
     3841 aagctggaga acctcatgct ggacaaggac gggcacatta agatcacaga cttcgggctg
     3901 tgcaaggagg ggatcaagga cggtgccacc atgaagacct tttgcggcac acctgagtac
     3961 ctggcccccg aggtgctgga ggacaatgac tacggccgtg cagtggactg gtgggggctg
     4021 ggcgtggtca tgtacgagat gatgtgcggt cgcctgccct tctacaacca ggaccatgag
     4081 aagctttttg agctcatcct catggaggag atccgcttcc cgcgcacgct tggtcccgag
     4141 gccaagtcct tgctttcagg gctgctcaag aaggacccca agcagaggct tggcgggggc
     4201 tccgaggacg ccaaggagat catgcagcat cgcttctttg ccggtatcgt gtggcagcac
     4261 gtgtacgaga agaagctcag cccacccttc aagccccagg tcacgtcgga gactgacacc
     4321 aggtattttg atgaggagtt cacggcccag atgatcacca tcacaccacc tgaccaagat
     4381 gacagcatgg agtgtgtgga cagcgagcgc aggccccact tcccccagtt ctcctactcg
     4441 gccagcggca cggccgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     4501 cccggcccta gtacatccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc
     4561 gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg
     4621 ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc
     4681 aagttgaata accggaaatg ctttcccgca gaacctgaag atgttcgcga ttatcttcta
     4741 tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac
     4801 atgcttcatc gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt
     4861 atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg
     4921 ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga tcgctgccag
     4981 gatatacgta atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa
     5041 attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat
     5101 attggcagaa cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg
     5161 gtaactaaac tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac
     5221 tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta
     5281 tcaactcgcg ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag
     5341 gatgactctg gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg
     5401 cgagatatgg cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc
     5461 aatgtaaata ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg
     5521 cgcctgctgg aagatggcga ttagaattcg atatcaagct tatcgataat caacctctgg
     5581 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5641 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5701 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5761 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5821 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5881 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5941 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6001 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6061 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6121 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagata
     6181 tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac
     6241 gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     6301 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     6361 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agggtcgacc
     6421 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     6481 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     6541 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
     6601 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
     6661 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg
     6721 ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa
     6781 gctggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6841 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6901 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     6961 gtgtagatcc ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     7021 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     7081 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     7141 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7201 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7261 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttact
     7321 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     7381 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     7441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8041 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8281 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8341 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8401 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8461 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8521 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8581 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8641 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     8701 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8761 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8821 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8881 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8941 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     9001 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9061 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9121 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9181 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9241 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9301 agaaaaataa acaaatagga gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9361 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9421 gtcttcaaga attagcttgg ccattgcata cgttgtatcc atatcataat atgtacattt
     9481 atattggctc atgtccaaca ttaccgccat gttgacattg attattgact agttattaat
     9541 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     9601 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     9661 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     9721 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     9781 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     9841 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     9901 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     9961 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
    10021 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
    10081 tctatataag cagagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc
    10141 cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
    10201 gaatcgtggt ctcgctgttc cttgggaggg tctcctctga gtgatt
//
