LOCUS       sequence-37184-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Waldner C, Rempel O, Schutte F, Yanik M, Solomentsew N, Ryffel GU
  TITLE     Double conditional human embryonic kidney cell line based on FLP and
            PhiC31 mediated transgene integration.
  JOURNAL   BMC Res Notes. 2011 Oct 18;4:420.
  PUBMED    22008483
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2011-10-18"; volume: "4"; pages: "420"
COMMENT     Sequence Label: pINT-PuroDDEYFP
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(30..350,351..353))
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     regulatory      350..359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(391..409)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(392..409)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ggaagctcct cgcccttgct cacgaattct tccggtttta gaagctccac atcgaagacg
       61 agagtggcat gtggtgggat gatgcctggg tgcccagtgg caccataggc ataatctgga
      121 gatatagtca gtttggctct ctgacccaca ctcatctggg caaccccttc ttcccagcct
      181 cggatcacct cctgcttgcc tagcataaac ttaaagggct tgtttctgtc ccgggaggaa
      241 tcgactttct ttccatcttc aagcatcccg gtgtagtgca ccacacaggt ctggccgcgc
      301 ttggggaagg tgcgcccgtc tcctggggag atggtttcca cctgcactcc catggtggcg
      361 gatcctgcaa aaagaacaag tagcttgtat tctatagtgt cacctaaatc aagcttgctc
      421 cgacgtcccc aggcagaatg gcggttccct aaacgagcat tgcttatata gacctcccat
      481 taggcacgcc taccgcccat ttacgtcaat ggaacgccca tttgcgtcat tgcccctccc
      541 cattgacgtc aatggggatg tacttggcag ccatcgcggg ccatttaccg ccattgacgt
      601 caatgggagt actgccaatg taccctggcg tacttccaat agtaatgtac ttgccaagtt
      661 actattaata gatattgatg tactgccaag tgggccattt accgtcattg acgtcaatag
      721 ggggcgtgag aacggatatg aatgggcaat gagccatccc attgacgtca atggtgggtg
      781 gtcctattga cgtcaatggg cattgagcca ggcgggccat ttaccgtaat tgacgtcaat
      841 gggggaggcg ccatatacgt caataggacc gcccatatga cgtcaatagg aaagaccata
      901 tatagagacc a
//
