LOCUS       sequence-371906-        5939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses Flag-tagged Ferritin-iBox-PAK4cat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Li TL, Wang Z, You H, Ong Q, Varanasi VJ, Dong M, Lu B, Pasca SP, 
            Cui B
  TITLE     Engineering a Genetically Encoded Magnetic Protein Crystal.
  JOURNAL   Nano Lett. 2019 Oct 9;19(10):6955-6963. doi: 
            10.1021/acs.nanolett.9b02266. Epub 2019 Sep 25.
  PUBMED    31552740
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.nanolett.9b02266"; journalName: "Nano Lett"; date: 
            "2019-10-9- 9"; volume: "19"; issue: "10"; pages: "6955-6963"
COMMENT     Sequence Label: pXJ40-Flag-Ferritin-iBox-PAK4cat
FEATURES             Location/Qualifiers
     source          1..5939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        9..312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        313..516
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     467..487
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     513..537
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          678..1250
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1258..1277
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1305..1324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1305..1323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1338..1361
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3032..3153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3069..3088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3123..3142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3187..3204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3358..3946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3438..3457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4117..4908,4909..4977))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4740..4759
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4978..5082)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5150..5168
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5206..5228)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5328..5347
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5414..5869)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5551..5572)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5763..5782
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctagagtccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
       61 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      121 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      181 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      241 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      301 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      361 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      421 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      481 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      541 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      601 cgcggccggg aacggtgcat tggaacggac ccctaggctt ttgcaaaaag ctccggatcg
      661 atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg
      721 taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat
      781 gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt
      841 gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta
      901 gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact
      961 ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt
     1021 tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg
     1081 gctggcgtgg aaatattctt attggtagaa acaactacat cctggtcatc atcctgcctt
     1141 tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca
     1201 aaccgggccc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1261 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgtaatac gactcactat
     1321 agggcgaatt caccatggac tacaaggacg acgatgataa gtccggatcc gccaccatgc
     1381 tgagcgagag gatgctgaag gccctgaacg accagctgaa cagggagctg tacagcgcct
     1441 acctgtactt cgccatggcc gcctacttcg aggacctggg cctggagggc ttcgccaact
     1501 ggatgaaggc ccaggccgag gaggagatcg gccacgcccc caggagctac aactacatct
     1561 acgacaggaa cggcagggtg gagctggacg agatccccaa gccccccaag gagtgggaga
     1621 gccccctgaa ggccttcgag gccgcctacg agcacgagaa gttcatcagc aagagcatct
     1681 acgagctggc cgccctggcc gaggaggaga aggactacag caccagggcc ttcctggagt
     1741 ggttcatcaa cgagcaggtg gaggaggagg ccagcgtgaa gaagatcctg gacaagctga
     1801 agttcgccaa ggacagcccc cagatcctgt tcatgctgga caaggagctg agcgccaggg
     1861 cccccaagct gcccggcctg ctgatgcagg gcggcgaggg atccgaagca gaggactgga
     1921 cagcagccct actgaacagg ggtcgcagtc gccagcccct ggtactaggg gacaattgct
     1981 ttgctgactt ggtgcacaac tggatggagc tgcctgagga attccccgcc gcccctgctg
     2041 ttcctgggcc ccctggcccc cgctcaccac agcgggagcc acagcgagta tcccatgagc
     2101 agttccgggc tgccctgcag ctggtggtgg acccaggcga cccccgctcc tacctggaca
     2161 acttcatcaa gattggcgag ggctccacgg gcatcgtgtg catcgccacc gtgcgcagct
     2221 cgggcaagct ggtggccgtc aagaagatgg acctgcgcaa gcagcagagg cgcgagctgc
     2281 tcttcaacga ggtggtaatc atgagggact accagcacga gaatgtggtg gagatgtaca
     2341 acagctacct ggtgggggac gagctctggg tggtcatgga gttcctggaa ggaggcgccc
     2401 tcaccgacat cgtcacccac accaggatga acgaggagca gatcgcagcc gtgtgccttg
     2461 cagtgctgca ggccctgtcg gtgctccacg cccagggcgt catccaccgg gacatcaaga
     2521 gcgactcgat cctgctgacc catgatggca gggtgaagct gtcagacttt gggttctgcg
     2581 cccaggtgag caaggaagtg ccccgaagga agtcgctggt cggcacgccc tactggatgg
     2641 ccccagagct catctcccgc cttccctacg ggccagaggt agacatctgg tcgctgggga
     2701 taatggtgat tgagatggtg gacggagagc ccccctactt caacgagcca cccctcaaag
     2761 ccatgaagat gattcgggac aacctgccac cccgactgaa gaacctgcac aaggtgtcgc
     2821 catccctgaa gggcttcctg gaccgcctgc tggtgcgaga ccctgcccag cgggccacgg
     2881 cagccgagct gctgaagcac ccattcctgg ccaaggcagg gccgcctgcc agcatcgtgc
     2941 ccctcatgcg ccagaaccgc accagatgac tcgaggcggc cgccccgggc tgcagggtac
     3001 cagatctgac tgactgatct tattaaagca gaacttgttt attgcagctt ataatggtta
     3061 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3121 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggtcgactc tagactcttc
     3181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4081 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4141 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4201 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4261 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4321 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4381 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4441 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4501 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4561 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4621 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4681 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4741 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4801 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4861 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4921 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4981 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5041 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5101 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     5161 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     5221 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     5281 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     5341 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     5401 ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     5461 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     5521 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     5581 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     5641 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     5701 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     5761 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     5821 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat
     5881 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccag
//
